Resistance large-scale accurate identification method for resisting brown plant-hopper in rice adult-plant stage

A rice brown planthopper and identification method technology, applied in the field of rice genetics and breeding, can solve the problems of low identification efficiency, few applications, poor identification result reliability, etc., and achieve the effects of improving breeding efficiency, accurate identification results, and shortening breeding cycle.

Active Publication Date: 2018-11-16
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The damage caused by brown planthoppers in rice production mostly occurs at the adult plant stage of rice, but the identification of resistance to brown planthopper at the adult plant stage of rice has always had problems such as low identification efficiency and poor reliability of identification results. There are not many applications such as construction, so it is of great practical significance to solve the technical problems of high-efficiency, rapid and accurate identification of rice adult plants

Method used

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  • Resistance large-scale accurate identification method for resisting brown plant-hopper in rice adult-plant stage
  • Resistance large-scale accurate identification method for resisting brown plant-hopper in rice adult-plant stage
  • Resistance large-scale accurate identification method for resisting brown plant-hopper in rice adult-plant stage

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Effect test

Embodiment 1

[0052] A method for large-scale and accurate identification of resistance to brown planthopper in rice at the adult plant stage, comprising the following steps:

[0053] (1) Prepare rice pile samples;

[0054] Plant the rice population that needs to be identified in the field, and use conventional planting methods to manage the rice in the field; peel off a rooted tiller from each rice plant of the population that needs to be identified at the adult stage, and require tillers that are peeled off from different plants The size is consistent, the growth is strong, and there is no disease and insect damage. Each tiller uses scissors to intercept the lower rice pile as an identification sample, and the length of the interception is 20cm;

[0055] (2) Samples of rice piles;

[0056] Rice piles are planted in rows in support basins filled with shallow soil, and the insect-susceptible variety TN1 is used as a control, and one row of the control variety is planted in each pot; when pla...

Embodiment 2

[0084] A method for large-scale and accurate identification of resistance to brown planthopper in rice at the adult plant stage, comprising the following steps:

[0085] (1) Prepare rice pile samples;

[0086] Plant the rice population that needs to be identified in the field, and use conventional planting methods to manage the rice in the field; peel off a rooted tiller from each rice plant of the population that needs to be identified at the adult stage, and require tillers that are peeled off from different plants The size is consistent, the growth is strong, and there is no disease and insect pest. Each tiller uses scissors to intercept the lower rice pile as an identification sample, and the length of the interception is 10cm;

[0087] (2) Samples of rice piles;

[0088] Rice piles were planted in rows in support basins filled with shallow soil, and the insect-susceptible variety 9311 was used as a control, and one row of the control variety was planted in each pot; when...

Embodiment 3

[0114] Technical Verification 3: Screening of Wild Rice Resistance Resources

[0115]Wild rice is an important resource in my country and contains many resistance genes, including the brown planthopper resistance gene with important utilization value. Screening resistance resources from wild rice can provide research materials for discovering new genes resistant to BPH. However, it is difficult to harvest wild rice seeds, and it is extremely inconvenient and inefficient to use seeds to identify resistance to BPH. In this experiment, the "rice pile identification method" was used to identify the brown planthopper in wild rice resources, and beneficial results were obtained.

[0116] A method for large-scale and accurate identification of resistance to brown planthopper in rice at the adult plant stage, comprising the following steps:

[0117] (1) Prepare rice pile samples;

[0118] Sampling and identification were taken from the common wild rice conservation nursery of Guang...

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Abstract

The invention discloses a resistance large-scale accurate identification method for resisting brown plant-hopper in a rice adult-plant stage, and belongs to the field of rice genetic and breeding. Theresistance large-scale accurate identification method for resisting the brown plant-hopper in the rice adult-plant stage comprises the following steps of (1) rice stubble sample preparation; (2) ricestubble sample planting; (3) excess insect inoculation identification; (4) management after insect inoculation; and (5) identification result assessment. According to the resistance large-scale accurate identification method for resisting the brown plant-hopper in the rice adult-plant stage, a 'rice stubble identification method' is established, and an efficient resistance identification technology is provided for breeding of rice resistance to the brown plant-hopper of various scales. In general, resistance breeding materials of large, medium and small sizes can be identified at a time and can also be identified repeatedly, a seedling period identification result in the adult-plant stage can be identified repeatedly, and the identification result is accurate, thereby effectively promoting the progress of a resistance genetic and breeding project.

Description

technical field [0001] The invention belongs to the field of rice genetics and breeding, and in particular relates to a method for large-scale and precise identification of rice adult plant resistance against brown planthopper. Background technique [0002] Rice is one of the main food crops in my country, and rice production is facing serious threats from brown planthopper. The rice brown planthopper is an explosive and harmful rice pest. At present, the rice planthopper (mainly brown planthopper) occurs in an area of ​​more than 20 million hectares in my country's rice areas every year, and the direct rice loss caused by the rice planthopper damage is more than 2.8 million tons. . For a long time, the control of brown planthoppers has mainly relied on the application of chemical insecticides. The large-scale application of insecticides often leads to the destruction of the ecological environment, the killing of a large number of natural enemies of brown planthoppers and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00A01K67/033
CPCA01G7/00A01K67/033
Inventor 李容柏韩悦王萱刘芳罗亮韦政覃宝祥
Owner GUANGXI UNIV
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