Resistance large-scale accurate identification method for resisting brown plant-hopper in rice adult-plant stage
A rice brown planthopper and identification method technology, applied in the field of rice genetics and breeding, can solve the problems of low identification efficiency, few applications, poor identification result reliability, etc., and achieve the effects of improving breeding efficiency, accurate identification results, and shortening breeding cycle.
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Embodiment 1
[0052] A method for large-scale and accurate identification of resistance to brown planthopper in rice at the adult plant stage, comprising the following steps:
[0053] (1) Prepare rice pile samples;
[0054] Plant the rice population that needs to be identified in the field, and use conventional planting methods to manage the rice in the field; peel off a rooted tiller from each rice plant of the population that needs to be identified at the adult stage, and require tillers that are peeled off from different plants The size is consistent, the growth is strong, and there is no disease and insect damage. Each tiller uses scissors to intercept the lower rice pile as an identification sample, and the length of the interception is 20cm;
[0055] (2) Samples of rice piles;
[0056] Rice piles are planted in rows in support basins filled with shallow soil, and the insect-susceptible variety TN1 is used as a control, and one row of the control variety is planted in each pot; when pla...
Embodiment 2
[0084] A method for large-scale and accurate identification of resistance to brown planthopper in rice at the adult plant stage, comprising the following steps:
[0085] (1) Prepare rice pile samples;
[0086] Plant the rice population that needs to be identified in the field, and use conventional planting methods to manage the rice in the field; peel off a rooted tiller from each rice plant of the population that needs to be identified at the adult stage, and require tillers that are peeled off from different plants The size is consistent, the growth is strong, and there is no disease and insect pest. Each tiller uses scissors to intercept the lower rice pile as an identification sample, and the length of the interception is 10cm;
[0087] (2) Samples of rice piles;
[0088] Rice piles were planted in rows in support basins filled with shallow soil, and the insect-susceptible variety 9311 was used as a control, and one row of the control variety was planted in each pot; when...
Embodiment 3
[0114] Technical Verification 3: Screening of Wild Rice Resistance Resources
[0115]Wild rice is an important resource in my country and contains many resistance genes, including the brown planthopper resistance gene with important utilization value. Screening resistance resources from wild rice can provide research materials for discovering new genes resistant to BPH. However, it is difficult to harvest wild rice seeds, and it is extremely inconvenient and inefficient to use seeds to identify resistance to BPH. In this experiment, the "rice pile identification method" was used to identify the brown planthopper in wild rice resources, and beneficial results were obtained.
[0116] A method for large-scale and accurate identification of resistance to brown planthopper in rice at the adult plant stage, comprising the following steps:
[0117] (1) Prepare rice pile samples;
[0118] Sampling and identification were taken from the common wild rice conservation nursery of Guang...
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