A kind of in vitro propagation and culture method of duck circovirus

Abstract

The invention provides a method for in vitro propagation and cultivation of duck circovirus, comprising the steps of: (1) collecting the blood of healthy adult muscovy ducks, carrying out separation and culture of PBMCs, and preparing PBMC cell suspensions; Cyclovirus-positive disease materials were added to PBMC cell suspension for culture. The invention provides a method for proliferating and culturing DuCV in vitro, the virus titer is greatly improved after inoculation and culture, the isolation and identification of the virus can be achieved in the laboratory, and the research on its pathogenic mechanism and harm provides a relatively large help.

Description

technical field [0001] The invention relates to an in vitro propagation and culture method of duck circovirus. Background technique [0002] Duck circovirus (Duckcircovirus, DuCV) is a new member of the Circovirus genus in the Circoviridae family. It can cause immunosuppression in ducks and cause harm to the duck industry. The clinical symptoms of ducks infected with duck circovirus DuCV are mainly poor development and obvious feather dystrophy, especially in the back spine (feathers). It is manifested as heterophilic inflammatory exudation of vesicles and tissues surrounding the vesicles. The general pathology of internal organs shows the symptoms of co-infection of duck infectious serositis. Histopathological examination of the bursa of Fabricius shows that lymphocyte damage, Necrosis, histiocytosis (Hattermann et al., 2003). [0003] DuCV was discovered in 2003. Since the virus does not cause visible lesions to host cells in most cases, the in vitro propagation of the v...

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Problems solved by technology

[0003] DuCV was discovered in 2003. Since the virus does not cause visible lesions to host cells in most cases, the in vitro propagation of the virus has not progressed. Up to now, there is no feasible method for in vitro propagation and cultivation of DuCV, which cannot be completed in the laboratory. Virus isolation and identification

Therefore, there are many difficulties in the research on its pathogenic mechanism and harm, and the development of vaccines, and the understanding is still very superficial.

However, the current epidemiological studies have shown that the disease is common in duck populations in my country. Drawing on the existing research results of other circoviruses, it is speculated that the existence of DuCV may increase the risk of duck plague, duck viral hepatitis, and duck infectious serosa. colibacillosis, duck pasteurellosis, Escherichia coli disease and other common duck diseases and aggravated its pathogenicity, so in today's rapid development of duck industry scale and quantity, it is urgent to propose a method of in vitro multiplication and cultivation of DuCV

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