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Application of soybean ZF-HD protein coding gene GmZFHD11

A ZF-HD, gene-encoding technology, applied in the field of genetic engineering, can solve the problem of less research on members of the ZFHD transcription factor family

Active Publication Date: 2018-11-20
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there are few related studies on the members of the ZFHD transcription factor family in soybean

Method used

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  • Application of soybean ZF-HD protein coding gene GmZFHD11
  • Application of soybean ZF-HD protein coding gene GmZFHD11
  • Application of soybean ZF-HD protein coding gene GmZFHD11

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Embodiment 2

[0038] The genetic engineering application of embodiment 2 gene GmZFHD11

[0039] 1) Cloning of soybean ZFHD protein coding gene GmZFHD11

[0040] Using the total RNA of the pod of soybean (Glycine max) Kefeng No. 1 variety as a template, the first strand of cDNA was synthesized by reverse transcription, and PCR amplification was carried out. The primer sequences are shown in SEQ ID NO.1 and SEQ ID NO.2. The PCR program is as follows: pre-denaturation at 95°C for 3 minutes, denaturation at 95°C for 15 seconds, annealing at 60°C for 15 seconds, extension at 72°C for 1 minute, a total of 35 cycles, and a final incubation at 72°C for 5 minutes, followed by constant temperature at 12°C to clone the PCR product To the CE Entry vector carrier, after sequencing, obtain the CDS sequence of the soybean GmZFHD11 gene with a length of 1077bp with a complete coding region, wherein the sequence of the coding region is shown in SEQ ID NO.1;

[0041] 2) Construction of plant expression vect...

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Abstract

The invention discloses an application of a soybean ZF-HD protein coding gene GmZFHD11. The nucleotide sequence of the soybean ZF-HD protein coding gene GmZFHD11 is SEQ ID NO. 1. By heterologous expression of a constructed plant over-expression vector pMDC83-GmZFHD11 in the wild type of Arabidopsis thaliana, the number of over-expressed plant root hair obviously increases and exceeds that of a wild type control group, indicating that the gene can be introduced into a plant as a target gene by increasing the number of root hair of the plant. Therefore, the soybean ZF-HD protein coding gene GmZFHD11 can be applied in improving the number of root hair of a plant through genetic engineering so as to affect the growth and the development of a transgenic plant.

Description

technical field [0001] The invention relates to the application of soybean ZF-HD protein coding gene GmZFHD11, which belongs to the field of genetic engineering. Background technique [0002] The homeobox domain (HD) is a 60-amino acid DNA-binding motif that exists in many transcription factors, mainly encoded by the homeobox gene, and plays an important role in the development of animals, plants and fungi effect. Homeobox domain proteins, as transcription factors, can bind to promoter sequences and affect the expression of mRNA. A typical HD structure includes 3 α-helices and 1 N-terminal turn helix. [0003] Zinc finger homeobox domain proteins (ZF-HD) belong to the family of transcription factors and regulate plant growth and development. ZF-HD contains a zinc finger domain at the N-terminus and a homeobox domain at the C-terminus, hence the name ZF-HD. ZFHD has been identified in many plants, such as Arabidopsis, rice, wheat and soybean. Four ZFHD genes that can bin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8261
Inventor 阚贵珍张鑫楠马玮域元文杰喻德跃
Owner NANJING AGRICULTURAL UNIVERSITY