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Method for preparing gamma-aminobutyric acid by taking lepidotrigla abyssalis as raw material

A technology of aminobutyric acid and matchmaker fish, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as environmental pollution, discarding, waste of resources, etc., to improve economic benefits, save production costs, reduce Effects of Environmental Pollution Problems

Inactive Publication Date: 2018-11-20
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of processing, the viscera of matchmaker fish with high protein content is often discarded, which not only causes waste of resources, but also pollutes the environment

Method used

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  • Method for preparing gamma-aminobutyric acid by taking lepidotrigla abyssalis as raw material
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  • Method for preparing gamma-aminobutyric acid by taking lepidotrigla abyssalis as raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Remove the head, tail, fin, bone and skin of fresh matchmaker fish raw material, mix the fish meat and viscera with a homogenizer and grind into a homogenate, add water (w / v) twice the volume of the homogenate to obtain a raw material solution ;

[0024] (2) Add 2.0% (w / v) glucose to the raw material solution, adjust the pH value to 6.5, and obtain the culture solution after high temperature and high pressure sterilization.

[0025] (3) After the obtained culture solution was cooled to room temperature, the activated Enterococcus avium suspension was inoculated with 1.0% amount (v / v), and then fermented and cultivated at 37° C. for 21 hours, so that Enterococcus avium entered a stable phase, and obtained The culture solution of Enterococcus avium (in the first stage, the optimum growth conditions of Enterococcus avium in the mixed medium of matchmaker fish meat and offal are: glucose addition 2.0%, pH value 6.5, culture temperature 37 ℃, culture time 21 hours). Ent...

Embodiment 2

[0030] (1) The viscera of the fresh matchmaker fish is taken from the raw material, ground into a homogenate with a homogenizer, and water (w / v) twice the volume of the homogenate is added to obtain a raw material solution;

[0031] (2) Add 2.0% (w / v) glucose to the raw material solution, adjust the pH value to 6.5, and obtain the culture solution after high temperature and high pressure sterilization.

[0032] (3) After the obtained culture solution is cooled to room temperature, after inoculating the activated Enterococcus avium bacterial suspension by 1.0% amount (v / v), ferment and cultivate at 37° C. for 21 hours, so that Enterococcus avium enters a stable phase, and obtains Culture medium of Enterococcus avium.

[0033] (4) Subsequently, 1.5% sodium glutamate was added to the culture solution rich in Enterococcus avium, the pH value was adjusted to 6.0, and it was fermented and cultivated at 37°C for 4 days to obtain a GABA-rich fermentation solution with a GABA concentra...

Embodiment 3

[0037] (1) After the head, tail, fin, bone and skin of the fresh matchmaker fish raw material are removed, the fish meat is ground into a homogenate with a homogenizer, and water (w / v) 5 times the mass of the homogenate is added to obtain a raw material solution;

[0038] (2) Add 1.0% (w / v) glucose to the raw material liquid, adjust the pH value to 5.5, and obtain the culture liquid after high temperature and high pressure sterilization.

[0039] (3) After the gained culture solution is cooled to room temperature, after inoculating the suspension of activated Lactobacillus brevis by 1.0% amount (v / v), ferment and cultivate at 37°C for 18 hours, so that Lactobacillus brevis enters a stable phase, (the first stage, the optimal growth conditions of Lactobacillus brevis in the matchmaker fish meat medium are: glucose addition 1.0%, pH value 5.5, culture temperature 37°C, culture time 18 hours) to obtain a culture solution rich in Lactobacillus brevis. (The fermentation strain Lactob...

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Abstract

The invention belongs to the technical field of biological processing of aquatic products and particularly relates to a method for preparing gamma-aminobutyric acid by taking lepidotrigla abyssalis asa raw material. The method comprises the following steps: taking lepidotrigla abyssalis meat and / or one or more of lepidotrigla abyssalis internal orangs as raw materials, adding a carbon source, adjusting the pH value to 4.5 to 7.0, inoculating activated bacterium suspension of a strain capable of generating glutamic acid decarboxylase, performing fermentation cultivation until the strain entersa stable stage, adding glutamate, adjusting the pH value and performing fermented cultivation to obtain fermentation liquid rich in GABA. By the method, the problem of environmental pollution causedby the waste internal organs of the lepidotrigla abyssalis can be solved and high-value utilization of fine processing of marine products is realized.

Description

technical field [0001] The invention belongs to the technical field of biological processing of aquatic products, and in particular relates to a method for preparing GABA from matchmaker fish. Background technique [0002] Matchmaker fish (gurnard) is a general term for fishes in the class Teleost, Scorpionidae, Gurnardidae, and the genus Gurnard. There are about 20 kinds. Body length up to 300mm. They are ruddy in color, slender and look like women, so they are called matchmaker fish. Matchmaker fish is distributed and produced in coastal areas of my country, and its resources are extremely rich. The meat of matchmaker fish has the advantages of high protein, low fat, rich in vitamins and minerals, good taste, and easy digestion and absorption. Therefore, its edible value is very high. In the process of processing, the internal organs of matchmaker fish with high protein content are often discarded, which not only causes waste of resources, but also pollutes the environ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C12R1/24C12R1/01C12R1/645
CPCC12P13/005
Inventor 李鹏程杨皓月邢荣娥刘松于华华秦玉坤李克成陈晓琳李荣锋
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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