Preparation method of L-phenylalanine molecularly imprinted polymer
A technology of phenylalanine and molecular imprinting, applied in the production of bulk chemicals, etc., can solve the problems of limited application, molecular imprinted polymer preparation and identification can not be well realized, and achieve high recognition, excellent resolution effect, The effect of simple method
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[0026] The preparation method of the L-phenylalanine molecularly imprinted polymer provided by the present invention comprises the following operations:
[0027] 1) Dissolve the template molecule L-phenylalanine, the functional monomer 1-vinyl-3-carbamoylmethylimidazolium chloride ionic liquid in the water-organic phase mixed solvent, and pre-polymerize in a refrigerator at 4°C 10~12h; wherein the molar ratio of L-phenylalanine: 1-vinyl-3-carbamoyl imidazole chloride is 1:4~6;
[0028] 2) Put the dispersant vinylpyrrolidone in the pre-polymerized mixed solution, heat up and stir until it is completely dissolved, and the amount of vinylpyrrolidone added is 10-20% of the mass of the functional monomer;
[0029] 3) Dissolve the crosslinking agent ethylene glycol dimethacrylate and the initiator azobisisobutyronitrile in the mixed solution in turn, and stir evenly, wherein L-phenylalanine: chlorinated 1-vinyl-3 -carbamoyl imidazole: the mol ratio of ethylene glycol dimethacrylate...
Embodiment 1
[0036] Weigh 66mgL-phenylalanine, 299mg chloride 1-vinyl-3-carbamoylmethylimidazolium ionic liquid is dissolved in 60mL water-ethanol (V 水 :V 乙醇 =1:3) in a mixed solvent, placed in a refrigerator at 4°C for prepolymerization for 12 hours, then added 30 mg of vinylpyrrolidone (PVP), heated and stirred until it was completely dissolved;
[0037] Then add 1.2mLEGDMA and 45mgAIBN in turn, stir evenly, degas by ultrasonic at a constant temperature of 25°C for 5min, then pass in nitrogen gas, and seal it up quickly after 15min. -1 Under the action of stirring, thermally polymerize in a water bath at 65°C for 24 hours to obtain white polymer particles;
[0038]After washing three times with 100 mL of distilled water and 100 mL of ethanol successively, and ultrasonically eluted with 200 mL of 0.6 mol / L NaCl solution for 10 min, the -1 Shake the shaker for 24 hours at a rotating speed to elute L-phenylalanine;
[0039] at 8500r·min -1 Centrifuge at high speed for ten minutes, take ...
Embodiment 2
[0044] Weigh 66mgL-phenylalanine, 374mg chloride 1-vinyl-3-carbamoylmethylimidazolium ionic liquid is dissolved in 60mL water-ethanol (V 水 :V 乙醇 =1:2) in a mixed solvent, put it in a refrigerator at 4°C for pre-polymerization for 12 hours, then add 37.6 mg of vinylpyrrolidone (PVP), heat up and stir until it completely dissolves;
[0045] Then add 1.2mLEGDMA and 48mgAIBN in turn, stir evenly, degas by ultrasonic at a constant temperature of 25°C for 5min, then pass in nitrogen gas for 15min, and then seal it quickly, at 450r·min -1 Under the action of stirring, thermally polymerize in a water bath at 65°C for 24 hours to obtain white solid particles;
[0046] After washing three times with 100 mL of distilled water and 100 mL of ethanol successively, and ultrasonically eluted with 200 mL of 0.6 mol / L NaCl solution for 10 min, the -1 Shake the shaker for 24 hours at a rotating speed to elute L-phenylalanine;
[0047] at 8500r·min -1 Centrifuge at high speed for ten minutes,...
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