DNA barcode, primer, kit, method and application

Abstract

The invention belongs to the field of species and strain identification, and specifically relates to a DNA sequence, a DNA barcode for identifying Blastobotrys adeninivorans, a primer, a kit, a methodand application. The DNA barcode originates from a genome of Blastobotrys adeninivorans TMCC 70007 and comprises the DNA sequence provided by the invention. The length of the DNA barcode is 500bp-2,200bp, preferably 500bp-1,600bp and more preferably 500bp-1,000bp. The DNA barcode can identify the Blastobotrys adeninivorans TMCC 70007 of Pu'er tea fermentation strains precisely, and can distinguish the strain from easily confused strains or other strains of the same species precisely and rapidly.

Description

technical field [0001] The invention belongs to the field of species and bacterial strain identification, and specifically relates to DNA barcodes, primers, kits, methods and applications. Background technique [0002] Pu-erh tea is a post-fermented tea with the geographical indication of Yunnan. It uses large-leaf sun-dried green tea as raw material and is made through a series of processes. The traditional production process of Pu'er tea is as follows: the picked fresh tea leaves are rolled, sun-dried, impurity-removed, tide-watered, heaped, dried, sieved, pressed and molded, and packaged for delivery. In the production of Pu-erh tea, the heaping fermentation process is the main factor to form the quality of Pu-erh tea. During this process, the internal components of the tea such as tea polyphenols, caffeine and some polysaccharides have undergone tremendous changes, making Pu-erh tea The special flavor, taste, quality and various health benefits of tea. [0003] In the ...

AI Technical Summary

Problems solved by technology

In recent years, due to the increasing market demand, many small-scale manufacturers lack systematic and in-depth research on Pu-erh tea, and wantonly abuse many other people's patents for profit. A series of methods, such as Pu’er tea processing and production, have greatly damaged the economic interests of some Pu’er tea production enterprises with relevant patents.

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