Application of noble dendrobium total alkali in preparing drug for atherosclerosis
A technology for atherosclerosis and Dendrobium candidum is applied in the directions of drug combinations, pharmaceutical formulations, plant raw materials, etc., and can solve problems such as complex pathological processes of atherosclerosis
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Embodiment 1
[0024] Embodiment 1, the preparation of Dendrobium nobile total alkaloids
[0025] 1. Experimental materials and instruments
[0026] 1.1. Medicinal materials and reagents
[0027] The medicinal material of Dendrobium nobile was purchased from Chishui Xintian Traditional Chinese Medicine Co., Ltd., and was identified as the dried stem of Dendrobium nobile Lindl. Glacial acetic acid (analytical grade, Shanghai Shenbo Chemical Co., Ltd., batch number 1202101), methyl red (C 15 h 15 N 3 o 2 : Shanghai Sanaisi Reagent Co., Ltd.), bromocresol green (C 21 h 14 Br 4 o 5 S: Shanghai Sanaisi Reagent Co., Ltd.), dichloromethane (Chongqing Chuanjiang Chemical Reagent Factory, batch number: 20030106), other alkaloid general color reagents (bismuth potassium iodide, dilute bismuth potassium iodide, improved bismuth potassium iodide ) are analytically pure, petroleum ether (30-60 ℃) and so on.
[0028] 1.2. Main instruments
[0029] Q-Exactive high-performance desktop quadrupole-...
Embodiment 2
[0033] Embodiment two, total alkaloids of Dendrobium nobile to ApoE - / - Prevention and treatment of hyperlipidemia and vascular atherosclerosis in mice
[0034] Objective: To observe the effect of Dendrobium nobile Lindl. Alkaloids (DNLA) on ApoE - / -Effects and mechanisms of hyperlipidemia and atherosclerosis in mice. Methods: Male C57BL / KsJ mice were used as normal controls, male ApoE mice - / - Mice were randomly divided into model control group, DNLA low, medium and high dose treatment groups (20, 30 mg, 80 mg / kg), simvastatin positive control group (50 mg / kg / d). After 16 weeks of continuous intragastric administration, the serum and blood vessel samples of mice in each group were collected, and the automatic biochemical instrument was used to measure triglyceride (TG), cholesterol, low-density lipoprotein, and high-density lipoprotein; For the condition of membrane plaques, the morphological changes of vascular tissue were observed by HE staining; the protein expression l...
Embodiment 3
[0178] Example 3. Effects of Total Alkaloids of Dendrobium Dendrobii on Rat Atherosclerosis Animal Model
[0179] Materials and methods
[0180] 1. Experimental materials
[0181] 1.1 Experimental animals Male SD rats, weighing 220-300 g, were provided by the Animal Center of Daping Hospital, Third Military Medical University, certificate number SCXK (Chongqing) 2007-0005.
[0182] 1.2 Main Drugs and Reagents
[0183] 1.2.1 Test drug: total alkaloids of Dendrobium nobile were provided by the Key Laboratory of Basic Pharmacology Education Ministry of Zunyi Medical College, content: 78.9%, batch number: 20150926); vitamin D3 (Beijing Suolaibao Biotechnology Co., Ltd., batch number: V8070); chloral hydrate (Sinopharm Chemical Reagent Co., Ltd.; batch number: 20071226); specification: 250g).
[0184] 1.2.2 Main reagents Rabbit anti-MCP-1, MCPIP, NF-κB polyclonal antibody (product of Beijing Boaosen Company), immunohistochemical detection kit (Gene Technology Shanghai Co., Ltd.,...
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