Application of noble dendrobium total alkali in preparing drug for atherosclerosis

A technology for atherosclerosis and Dendrobium candidum is applied in the directions of drug combinations, pharmaceutical formulations, plant raw materials, etc., and can solve problems such as complex pathological processes of atherosclerosis

Inactive Publication Date: 2018-11-27
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pathological process of atherosclerosis is complex. There have been many theories in the academic circles, such as oxidative stress and lipid infiltration theory, etc., whic...

Method used

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  • Application of noble dendrobium total alkali in preparing drug for atherosclerosis
  • Application of noble dendrobium total alkali in preparing drug for atherosclerosis
  • Application of noble dendrobium total alkali in preparing drug for atherosclerosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the preparation of Dendrobium nobile total alkaloids

[0025] 1. Experimental materials and instruments

[0026] 1.1. Medicinal materials and reagents

[0027] The medicinal material of Dendrobium nobile was purchased from Chishui Xintian Traditional Chinese Medicine Co., Ltd., and was identified as the dried stem of Dendrobium nobile Lindl. Glacial acetic acid (analytical grade, Shanghai Shenbo Chemical Co., Ltd., batch number 1202101), methyl red (C 15 h 15 N 3 o 2 : Shanghai Sanaisi Reagent Co., Ltd.), bromocresol green (C 21 h 14 Br 4 o 5 S: Shanghai Sanaisi Reagent Co., Ltd.), dichloromethane (Chongqing Chuanjiang Chemical Reagent Factory, batch number: 20030106), other alkaloid general color reagents (bismuth potassium iodide, dilute bismuth potassium iodide, improved bismuth potassium iodide ) are analytically pure, petroleum ether (30-60 ℃) and so on.

[0028] 1.2. Main instruments

[0029] Q-Exactive high-performance desktop quadrupole-...

Embodiment 2

[0033] Embodiment two, total alkaloids of Dendrobium nobile to ApoE - / - Prevention and treatment of hyperlipidemia and vascular atherosclerosis in mice

[0034] Objective: To observe the effect of Dendrobium nobile Lindl. Alkaloids (DNLA) on ApoE - / -Effects and mechanisms of hyperlipidemia and atherosclerosis in mice. Methods: Male C57BL / KsJ mice were used as normal controls, male ApoE mice - / - Mice were randomly divided into model control group, DNLA low, medium and high dose treatment groups (20, 30 mg, 80 mg / kg), simvastatin positive control group (50 mg / kg / d). After 16 weeks of continuous intragastric administration, the serum and blood vessel samples of mice in each group were collected, and the automatic biochemical instrument was used to measure triglyceride (TG), cholesterol, low-density lipoprotein, and high-density lipoprotein; For the condition of membrane plaques, the morphological changes of vascular tissue were observed by HE staining; the protein expression l...

Embodiment 3

[0178] Example 3. Effects of Total Alkaloids of Dendrobium Dendrobii on Rat Atherosclerosis Animal Model

[0179] Materials and methods

[0180] 1. Experimental materials

[0181] 1.1 Experimental animals Male SD rats, weighing 220-300 g, were provided by the Animal Center of Daping Hospital, Third Military Medical University, certificate number SCXK (Chongqing) 2007-0005.

[0182] 1.2 Main Drugs and Reagents

[0183] 1.2.1 Test drug: total alkaloids of Dendrobium nobile were provided by the Key Laboratory of Basic Pharmacology Education Ministry of Zunyi Medical College, content: 78.9%, batch number: 20150926); vitamin D3 (Beijing Suolaibao Biotechnology Co., Ltd., batch number: V8070); chloral hydrate (Sinopharm Chemical Reagent Co., Ltd.; batch number: 20071226); specification: 250g).

[0184] 1.2.2 Main reagents Rabbit anti-MCP-1, MCPIP, NF-κB polyclonal antibody (product of Beijing Boaosen Company), immunohistochemical detection kit (Gene Technology Shanghai Co., Ltd.,...

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Abstract

The invention discloses an application of noble dendrobium total alkali in preparing a drug for atherosclerosis and belongs to the technical field of biomedicine. According to the application of nobledendrobium total alkali in preparing the drug for atherosclerosis, the noble dendrobium is biennial noble dendrobium and the dosage of the noble dendrobium total alkali is 20-180 mg/kg per day. The invention further explicits the degreasing action of noble dendrobium total alkali DNLA and a probable action mechanism thereof. By adopting an experimental rat atherosclerosis model and an ApoE-/- mouse transgenic model, influence of the DNLA on hyperlipidemia atherosclerosis of blood vessels is observed, and the action mechanism thereof is analyzed, finding that the DNLA has preventing and treating action on the rat atherosclerosis model and the action mechanism is partially related to down-regulation of expression of MCP-1/CCR2, MCPIP and CRP genes, thereby providing a basic pharmacologicalbasis for clinical treatment of HLP and As by DENLA.

Description

technical field [0001] The invention relates, in particular, to the application of total alkaloids of Dendrobium nobile in the preparation of atherosclerotic drugs. Background technique [0002] With people's increasing living standards, continuous improvement of diet, and the development of population aging, cardiovascular and cerebrovascular diseases have become the primary cause of harm to human health, among which hyperlipidemia (HLP) is leading to atherosclerosis (atherosclerosis, AS) and the main and predisposing factors of cardiovascular and cerebrovascular diseases, and it has attracted more and more attention. [0003] A large number of research data in the past ten years have shown that the incidence of hyperlipidemia in China is increasing year by year, and the damage to human health is mainly manifested in the damage of the cardiovascular system, especially the damage to the heart, brain, and kidney blood vessels. Hyperlipidemia (hyperlipidaemia, HLP) refers to ...

Claims

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Application Information

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IPC IPC(8): A61K36/8984A61P9/10
CPCA61K36/8984A61P9/10
Inventor 石京山吴芹李利生陆远富徐云燕陈晶
Owner ZUNYI MEDICAL UNIVERSITY
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