Method for detecting gene with plant flavonoid biosynthesis positive regulation function

A biosynthesis and positive regulation technology, applied in the field of genetic engineering, can solve problems such as inability to clarify, and achieve the effects of shortening detection time, less time, and easy operation.

Inactive Publication Date: 2018-11-27
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0003] In order to solve the problem in the prior art that it is impossible to clarify which ARF genes are involved in the biosynthesis of plant flavonoids, the present invention provides a method for detecting genes that positively regulate the biosynthesis of plant flavonoids. The purpose of realization is , can accurately determine which genes in the specific ARF family have the function of positively regulating the biosynthesis of plant flavonoids, clarified the gene network of auxin regulating flavonoid biosynthesis, and used the detected genes to cultivate plants with higher content. plant species of flavonoids offer the possibility

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  • Method for detecting gene with plant flavonoid biosynthesis positive regulation function
  • Method for detecting gene with plant flavonoid biosynthesis positive regulation function
  • Method for detecting gene with plant flavonoid biosynthesis positive regulation function

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Embodiment 1

[0024] Embodiment 1: The plant described in the present invention is Arabidopsis thaliana.

[0025] A method for detecting genes that positively regulate biosynthesis of plant flavonoids disclosed by the present invention comprises the following steps:

[0026] (1) Screen out candidate genes involved in the positive regulation of plant flavonoid biosynthesis in the ARF gene: analyze the gene expression patterns of key structural genes and transcriptional regulatory genes in the plant flavonoid biosynthesis pathway during plant seed coat development, and then Analyze the gene expression patterns of all ARF genes during plant seed coat development, and compare the key structural genes and transcriptional regulatory genes in the plant flavonoid biosynthesis pathway with the gene expression patterns of ARF genes during plant seed coat development , combined with the analysis of the evolutionary relationship of the ARF gene, the candidate genes whose gene expression pattern in the ...

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Abstract

The invention discloses a method for detecting a gene with a plant flavonoid biosynthesis positive regulation function. The method is characterized by including the steps: (1) screening out a candidate gene participating in plant flavonoid biosynthesis positive regulation from ARF genes; (2) verifying the plant flavonoid biosynthesis function of the candidate gene, and finally determining a targetgene. The method has the advantages that research methods of molecular biology, genetics and metabolite analysis are comprehensively utilized to determine a target gene and a key transcription factorregulated by an At5g62000 gene in biosynthesis ways of flavonol and proanthocyanidin (flavonoid metabolites), and a gene network of flavonoid biosynthesis regulated by auxin is expounded. The gene can be possibly used for culturing plant varieties with higher content of proanthocyanidin and flavonol.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to a method for detecting genes with the function of positively regulating biosynthesis of plant flavonoids. Background technique [0002] Flavonoids are a large class of secondary metabolites with important activities that widely exist in plants. Flavonoids play an important role in the growth, development and reproduction of plants; at the same time, they are also involved in the regulation of biotic and abiotic stress in plants; in addition, they also have anti-inflammatory, anti-cancer and cardiovascular and cerebrovascular Diseases and other effects that are beneficial to human health. Auxin is involved in the regulation of flavonoid biosynthesis, regulating multiple structural genes and transcription factors of flavonoid metabolic pathways. When Arabidopsis was treated with auxin IAA, the expression levels of key structural genes CHS, CHI, F3'H, and FL...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13
Inventor 江文波庞永珍
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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