Rapid classification and identification method for sea foods in close genetic relationship

A rapid classification and identification method technology, which is applied in the fields of analytical chemistry, mass spectrometry and rapid analysis, can solve the problems that scientific methods are yet to be developed, and achieve rapid classification and identification, and the effect of simple and easy methods

Inactive Publication Date: 2018-11-27
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Scientific methods specifically for the classification and identification of closely related seafood have yet to be developed

Method used

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  • Rapid classification and identification method for sea foods in close genetic relationship
  • Rapid classification and identification method for sea foods in close genetic relationship
  • Rapid classification and identification method for sea foods in close genetic relationship

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Examples of detection and identification:

[0055] Step a, collecting samples;

[0056] This embodiment takes the identification of large yellow croaker and small yellow croaker as an example. These two fishes are very similar in appearance and are easy to confuse, but the economic value of the two fishes is very different.

[0057] (1) Take the skins of large yellow croaker and small yellow croaker as samples to be tested, and remove the subcutaneous tissue and scales for later use.

[0058] (2) Take 0.2g of two kinds of fish skins respectively, add 2mL of 1.3mol / L sodium hydroxide solution to each fish skin, put in a 15ml centrifuge tube and shake overnight at room temperature, adjust the HS-3 vertical mixer to the maximum shaking speed, and then Take 150 μL of the solution and adjust the pH value to neutral or slightly acidic with 6 mol / L HCl solution.

[0059] (3) Using Thermo Scientific C18 Spin Column is used for desalination treatment. (4) Sample detection, ...

Embodiment 2

[0067] blind identification

[0068] The unknown fish was compared and identified with short snout sole, narrow tongue sole, jiaoshi tongue sole, half smooth tongue sole and the large yellow croaker and small yellow croaker in the examples.

[0069] Firstly, the skins of unknown fish and tongue sole, tongue sole narrow body, tongue sole Jiaoshi and half smooth tongue sole were taken as the samples to be tested, and they were soaked in sodium hydroxide solution respectively.

[0070] Take a small amount (100 to 200 microliters) of the processed sample. Use hydrochloric acid solution to adjust the pH to neutral or slightly acidic; figure 1 shown).

[0071] Step b, sample detection, is detected by matrix-assisted laser desorption ionization mass spectrometry.

[0072] Add the sample obtained in step a dropwise on the MALDI target plate, use SA as the matrix, and perform mass spectrometry detection.

[0073] In step c, 6 pieces of the same fish are taken as skin samples, and e...

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Abstract

The invention provides a rapid classification and identification method for sea foods in a close genetic relationship and aims to classify and identify different sea foods in close species. The methodcomprises the following steps: taking a part of meat and skin of sea foods as samples to be tested; treating the samples to be tested in an alkali solution; adjusting the solution to be neutral or weak acid; desalting the solution; detecting the solution with a mass spectrum, and recording spectrum signals; carrying out chemometric analysis on the acquired spectrum signals, thereby distinguishingdifferent sea foods. The method provided by the invention is simple and feasible, is capable of achieving scientific identification on sea foods of a same specie within hours on the basis of sample pretreatment, and is good in resolution and high in sensitivity.

Description

technical field [0001] The invention relates to the fields of analytical chemistry, mass spectrometry and rapid analysis, and provides an analysis method that can be used for rapid classification and identification of seafood. Background technique [0002] Fraudulent behavior driven by economic interests in the aquatic industry is a global problem. Among them, food mislabeling is common. Take aquatic products as an example, including replacing expensive seafood with low-cost seafood, replacing endangered species with normal legal seafood, Substituting normal seafood for seafood that needs to be tested for toxins and allergies can have a negative impact on the economy and health of consumers, as well as the ecological environment of the world's oceans, lakes and rivers. A random survey of commercially available fish samples in 19 countries showed that the average mislabelled fish species was as high as 25%, among which the mislabeled rate in China and Turkey was as high as 86...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 毕红燕谢晶
Owner SHANGHAI OCEAN UNIV
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