Detection card, detection box of cryptococcus and preparation method of detection card and box

A detection card, cryptococcus technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of simple operation, fast detection speed and strong specificity

Inactive Publication Date: 2018-11-27
广州爱康生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there is no mature crypt...

Method used

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  • Detection card, detection box of cryptococcus and preparation method of detection card and box
  • Detection card, detection box of cryptococcus and preparation method of detection card and box
  • Detection card, detection box of cryptococcus and preparation method of detection card and box

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Such as figure 1 As shown, an embodiment of the cryptococcal detection card of the present invention includes a rubber plate 1, on which a sample pad 2, a gold standard pad 3, and a chromatographic membrane are sequentially arranged end-to-end in accordance with the flow direction of the sample. 4 and the first water-absorbing pad 5, a second water-absorbing pad 6 is provided between the chromatographic membrane 4 and the rubber sheet 1, a detection area 7 and a quality control area 8 are provided on the chromatographic membrane 4, and the detection area 7 is set on the sample pad 2 Between the gold standard pad 3 and the quality control area 8, the first monoclonal antibody of colloidal gold-labeled anti-cryptococcus capsular polysaccharide is adsorbed; the detection area is coated with the second monoclonal antibody of anti-cryptococcus capsular polysaccharide , or the side of the detection area 7 close to the sample pad is provided with a detection line 9, and the de...

Embodiment 2

[0042] Such as figure 2 As shown, an embodiment of the cryptococcus detection box of the present invention includes a box body 11, the detection card of embodiment 1 is arranged inside the box body 11, and the surface of the box body 11 is provided with a sample hole 12 and an observation window 13, Below the sample injection hole 12 is a sample pad (not shown in the figure), below the observation window 13 is a chromatographic membrane (not shown in the figure), and the box body 11 is provided with a detection card slot 14 .

Embodiment 3

[0044] A kind of embodiment of the preparation method of the cryptococcal detection card of the present invention comprises the following steps:

[0045] (1) Colloidal gold-labeled anti-CG (cryptococcus)-GXM (capsular polysaccharide) monoclonal antibody-1 (ie the first monoclonal antibody)

[0046] Colloidal gold was prepared by the classic trichloroauric acid-citric acid three sodium method, the particle size was 30nm, and the measured pH was 6.6; company) coated with colloidal gold, the coating concentration is 0.05mg / mL, and the free colloidal gold particles are separated by centrifugation, and then resuspended with 1 / 5 of the original volume of phosphate buffer (containing 1mg / mL PEG6000, 5% sucrose, 10 mM pH 7.2).

[0047] (2) Preparation of capsular polysaccharide antigen immune colloidal gold detection card

[0048] 1) Use the pretreatment buffer to pretreat the gold standard pad, soak the gold standard pad in the gold standard pad pretreatment buffer for 2 hours, tak...

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PUM

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Abstract

The invention discloses a cryptococcus detection card. The card comprises a rubber plate, wherein a sample pad, a gold mark pad, a chromatographic film and a first water absorption pad are sequentially arranged on the rubber plate end to end along the sample flowing direction; a second water absorption pad is arranged between the chromatographic film and the rubber plate; a detection area and a quality control area are arranged on the chromatographic film; and a colloidal gold marked first monoclonal antibody for resisting cryptococcus capsular polysaccharide is adsorbed on the gold mark pad.The detection card or detection box can be adopted to quickly and sensitively detect the existence of cryptococcus capsular polysaccharide antigen, and determine whether a detected object is affectedby cryptococcus or not. The detection card or detection box has high sensitivity, strong specificity and high detection speed, can give a detection result in five minutes, and is simple in operation.

Description

technical field [0001] The invention relates to the technical field of cryptococcus detection, in particular to a cryptococcus detection card, a detection box and a preparation method thereof. Background technique [0002] Cryptococcus is widely distributed in the natural environment, pigeon feces is the main source of infection, and Cryptococcus neoformans and Gothic can cause human cryptococcosis. Cryptococcosis can infect any tissue and organ in the human body. According to clinical manifestations, it can be divided into five types: pulmonary cryptococcosis, central nervous system cryptococcosis, skin and mucous membrane cryptococcosis, bone cryptococcosis and visceral cryptococcosis. The prognosis of cryptococcal infection is poor, and the mortality rate of central nervous system cryptococcosis is high. [0003] Cryptococcal infection in the normal population may be asymptomatic. When the body's immunity is low or suppressed, pathogenic bacteria can directly invade and...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558
CPCG01N33/56961G01N33/558
Inventor 刘灵辉赖演媚赖柔贝罗晓锋
Owner 广州爱康生物技术有限公司
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