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Tissue culture and rapid propagation method of camellia nitidissima

A tissue culture, golden camellia technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as the inability to rapidly and effectively expand the population of golden camellia, achieve improved disinfection effect, enhance tolerance, promote Growth and stretching effects

Inactive Publication Date: 2018-11-30
吕诗林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a method for rapid propagation of Camellia japonica through tissue culture, which uses the young and tender stem tips of Camellia japonica to quickly obtain Camellia japonica seedlings by means of tissue culture. This method can effectively improve the proliferation coefficient of Camellia japonica, reaching 4.8 -5.0 times, the rate of deformed seedlings is as low as 0.6%, the survival rate of seedlings is as high as 94.1%, and the average plant height of seedlings is 92mm, which effectively solves the problem that traditional breeding methods cannot quickly and effectively expand the population of Camellia japonica, and provides protection for Camellia japonica a new path

Method used

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  • Tissue culture and rapid propagation method of camellia nitidissima

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Embodiment 1

[0024] A tissue culture rapid propagation method of golden camellia, comprising:

[0025] Step 1. Cut the young stem tips of Camellia sinensis, soak the stem tips with an aqueous detergent solution with a mass fraction of 2% for 30 minutes, take them out, rinse them with running water for 10-15 minutes, and then place them in a pre-paste solution. In the flexible packaging bag of the mixed solution of starch and nano-titanium dioxide, immersed and sealed, the flexible packaging bag was placed in an ultra-high pressure container, treated at 80 MPa for 6s, and the stem tip was taken out from the flexible packaging bag. Rinse with water 6-8 times and absorb the water on the surface with sterile filter paper to obtain explants;

[0026] Step 2: Cut the explants into 1-2mm pieces and transfer them to the clump bud induction medium. First, culture them at 22-26°C for 2 days under dark conditions, and then at a culture temperature of 22-26°C with light. Intensity 1500lux, light time...

Embodiment 2

[0030] A tissue culture rapid propagation method of golden camellia, comprising:

[0031] Step 1. Cut the young stem tips of Camellia sinensis, soak the stem tips with an aqueous detergent solution with a mass fraction of 2% for 30 minutes, take them out, rinse them with running water for 10-15 minutes, and then place them in a pre-paste solution. In the flexible packaging bag of the mixed solution of starch and nano-titanium dioxide, immersed and sealed, the flexible packaging bag was placed in an ultra-high pressure container, treated at 85MPa for 4s, and the stem tip was taken out from the flexible packaging bag. Rinse with water 6-8 times and absorb the water on the surface with sterile filter paper to obtain explants;

[0032] Step 2: Cut the explants into 1-2mm pieces and transfer them to the clump bud induction medium. First, culture them at 22-26°C for 2 days under dark conditions, and then at a culture temperature of 22-26°C with light. The intensity of 1550lux, the ...

Embodiment 3

[0036] A tissue culture rapid propagation method of golden camellia, comprising:

[0037] Step 1. Cut the young stem tips of Camellia sinensis, soak the stem tips with an aqueous detergent solution with a mass fraction of 2% for 30 minutes, take them out, rinse them with running water for 10-15 minutes, and then place them in a pre-paste solution. In the flexible packaging bag of the mixed solution of starch and nano-titanium dioxide, immersed and sealed, the flexible packaging bag was placed in an ultra-high pressure container, treated at 90 MPa for 1 s, and the stem tip was taken out from the flexible packaging bag. Rinse with water 6-8 times and absorb the water on the surface with sterile filter paper to obtain explants;

[0038] Step 2: Cut the explants into 1-2mm pieces and transfer them to the clump bud induction medium. First, culture them at 22-26°C for 2 days under dark conditions, and then at a culture temperature of 22-26°C with light. Intensity 1600lux, light tim...

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Abstract

The invention discloses a tissue culture and rapid propagation method of camellia nitidissima. The method includes: transferring camellia nitidissima cluster buds into a rooting and strong seedling medium for culture to obtain camellia nitidissima seedlings. Specifically, the rooting and strong seedling medium comprises an upper medium and a lower medium, the upper medium is composed of: 3 / 4MS+2.5-3.0mg / L NAA+1.0-1.5mg / L LFS+1.4-1.8mg / L 4-CPA+1-5g / L goat horn carbide powder; and the lower medium is composed of: 1 / 2MS+1.0-1.5mg / L NAA+0.5-1.0mg / L 2, 4-D+0.5-0.8mg / L MET+1.0-1.5mg / L choline chloride+15-20g / L goat horn carbide powder. The method provided by the invention can effectively improve the multiplication coefficient of camellia nitidissima, and provides a new path for protection of camellia nitidissima.

Description

technical field [0001] The present invention relates to the field of tissue culture. More specifically, the present invention relates to a tissue culture rapid propagation method of Camellia sinensis. Background technique [0002] Camellia sinensis belongs to the Camellia family and the genus Camellia, and is as famous as the precious "living plant fossils" such as silver fir, cypress, dove tree, etc. It is a plant species in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora, and it is called the magical Oriental in foreign countries. Magic tea, known as "plant world giant panda", "tea queen". Camellia oleifera can be propagated by traditional sowing, cutting and grafting methods, but none of the above methods can rapidly and effectively expand the population of Camellia oleifera. How to quickly propagate golden camellia has become an urgent problem to be solved in the protection of golden camellia. SUMMARY OF THE INVENTIO...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 吕诗林
Owner 吕诗林