A plant-derived algastatic agent for inhibiting the growth of yellow hair algae and its preparation method and application
A technology of plant sources and inhibitors, applied in botany equipment and methods, biocides, plant growth regulators, etc., to achieve the effect of obvious decrease in cell density, cheap price, and simple preparation process
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Embodiment 1
[0027] The bloom algae aimed at in this embodiment is Xanthophyllum, which is a common species in lakes, rivers, and ponds in my country. The initial density of Xanthophyllum algae cells in the culture container is 1.02×10 6 individual / mL.
[0028] (1) The preparation method of plant-source Xanthophyllium algae inhibitor: wash, pulverize and sieve the littered leaves of sycamore sycamore, put them in a custom-made sieve silk bag (200 mesh), place them in a beaker, press 1 : Add distilled water at a solid-to-liquid ratio of 20, ultrasonically extract for 1h, and centrifuge (4000r / min) for 10min to get the supernatant and concentrate it to 250ml to obtain an extract with a concentration of 0.1g DW / ml. Plant-derived algastatics for growth.
[0029] (2) the using method of plant source Xanthophyllum inhibitor: the culture vessel used in the experiment is the triangular flask of volume 500ml, and the culture fluid volume that contains Xanthophyllum is 180ml, adds the plant source ...
Embodiment 2
[0031] The bloom algae targeted in this example is Xanthophyllum, which is a common species in lakes, rivers, and ponds in my country. The initial density of Xanthophyllum algae cells in the culture container is also 1.02×10 6 individual / mL.
[0032] (1) The preparation method of plant-derived algae inhibitor of yellow hair algae: same as embodiment 1.
[0033] (2) the using method of plant source Xanthophyllum inhibitor: the culture vessel used in the experiment is the triangular flask of volume 500ml, and the culture fluid volume that contains Xanthophyllum is 180ml, adds the plant source inhibitor that makes in the step (1), During the experiment, they were divided into two groups. In group A, 2.75 mL of plant-sourced algae inhibitors were added to the culture container of the experimental group. In group B, the plant-source inhibitor was replaced by 2.75 mL of distilled water, and other conditions remained unchanged; Then group A and group B were put into the light incuba...
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