Novel use of Polymerase I inhibitor
A technology of inhibitor and immunosuppressant, applied in the field of biomedicine, can solve the problem that the effect of CX-5461 has not been reported, and achieve the effect of inhibiting the proliferation of T cells and having great application prospects.
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[0047] Example 1 Cx5461 affects T cell activation by inhibiting the proliferation of T cells
[0048] Dissolve Cx5461 in dimethyl sulfoxide (DMSO) to make a Cx5461 solution and perform the following experiments:
[0049] 1. Inoculate Jurkat T cells in a six-well plate of RPMI1640 medium containing 10% fetal bovine serum, and divide them into four groups.
[0050] (1) Stimulation group (P / I): Add PMA / Ionmycin solution to each well;
[0051] (2) Positive control group (P / I+FK506): Add FK506 solution to each well until the concentration of FK506 is 1×10 -6 M;
[0052] (3) Experimental group (P / I+Cx5461): Add Cx5461 solution to each well until the concentration of Cx5461 is 1×10 -6 M;
[0053] (4) Control group (Con): add equal volume of DMSO to each well;
[0054] The above four groups were cultured at 37°C for 6 hours and then subjected to immunofluorescence experiments. Suspend Jurkat T cells in 50 mL culture medium, install filter paper and fixed glass slide on the cell spinner, then add...
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[0077] Example 2 Cx5461 delayed the rejection of skin allograft at the level of animal experiments
[0078] Take 2 g of Cx5461 and dissolve it in 1 mL of DMSO liquid. After the dissolution is complete, add physiological saline to make up the volume so that the final concentration of Cx5461 is 0.5 g / L, 1.0 g / L, 2.0 g / L to obtain the Cx5451 solution. experiment:
[0079] 1. Mouse skin transplantation experiment
[0080] (1) Establishment of mouse skin transplantation model
[0081] The laboratory purchased C57BL / 6 and C57BL / 6 at the age of 6-8 weeks in the Guangdong Medical Experimental Animal Center and kept them in the breeding room of the Shenzhen Graduate School of Tsinghua University. Two days before the operation, the recipient's C57BL / 6 mice were depilated; BALB / c mice and C57BL / 6 mice were anaesthetized with 10% sodium pentobarbital, and 75% ethanol was used to treat the donor and The back skin of the recipient mice was disinfected. The back skin of the donor mouse was remove...
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