The invention discloses a method for creating a cytoplasmic male sterile line of flowering Chinese cabbage, which comprises the following steps of: (1)
selfing Zhengcheng tip leaf flowering Chinese cabbage to find a sterile
plant; (2) carrying out
test cross on sterile plants and sister plants appearing in the Zhengcheng sharp-leaf flowering cabbage inbred line to obtain
test cross seeds; (3) planting the test-cross seeds, observing
fertility and nectary conditions in a flowering period,
backcrossing sterile plants and corresponding sister plants in a group with the test-cross
offspring sterility rate of more than 50%, screening out a group with the
sterility rate of 100% from backcross offsprings, and
selfing the sterile plants in the group with the corresponding sister plants to obtain the sister plants with the
infertility rate of more than 50% and the
infertility rate of more than 100% in the group with anther degradation and normal pistil and nectary.
Selfing the fertile sister plants to obtain
offspring to form a
maintainer line, and performing test crossing on the sterile plants and the
maintainer line to obtain a matched sterile line. According to the method, transformation of a sterile source is not needed, time is saved, cost is low, the sterile line preserves completeness of nectary genes, nectary is normal, the podding rate is high, the maturing rate is high, and the seed yield is high.