71 results about "Choy sum" patented technology

By means of plant gene engineering, primer is designed from No. 901 base to No. 1338 base inside the fertility relevant gene, 438 bp sequence is amplified as anti-sense gene segment and introduced into plant chromosome to obtain regenerative plant and artificial male sterile common Chinese cabbage and core. The said method is suitable for rape genus plants and may be used in fast constituting the male sterility line of different Chinese cabbage and similar vegetable crops without the limitation similar to that of seeking natural sterile source. The present invention opens one new way the wide application of plant gene engineering technology in agricultural production.

The invention discloses a breeding, reproduction and seed production method for a cytoplasmic male sterile line of a Chinese flowering cabbage. The breeding, reproduction and seed production method comprises the following steps: (1) taking a leaf mustard type oilseed rape cytoplasmic male sterility material hau CMS as a female parent and taking a Chinese flowering cabbage conventional variety brassica parachinensis bariley as a male parent; carrying out distant hybridization; separating hybridized strains and carrying out duplicitas rescuing culture; transplanting to a large field to obtain a species hybrid F1; and (2) selecting a sterile strain in the species hybrid F1 as a female parent and taking the brassica parachinensis bariley as a recurrent parent to continuously backcross for 5 generations to obtain the cytoplasmic male sterile line 1301A of the Chinese flowering cabbage. The cytoplasmic male sterile line of the Chinese flowering cabbage obtained by breeding has the characteristics that the sterility is stable and complete and is not influenced by environment, and leaves are not yellowed and the like; continuous planting of 5 generations to 10 generations is carried out and fertility identification is carried out; the sterility degree and the sterile strain rate of the sterile line are 100%.

The invention discloses a method for restraining bud flowering after Brassica campestris is picked. The method comprises the following steps of performing picking; performing treatment with hormones;performing treatment with illumination at time intervals; and performing storage. The brassica campestris which has 5-8 leaves and just bolts is used as raw materials, so that the requirement for thecondition that the raw materials are picked, is reduced, and loss caused by mechanical damage and yellowing and deterioration in the storage and transportation process of the raw materials can be avoided; low temperature and plant hormones are in cooperation, an illumination and shielding time-interval culturing technique is adopted, and the physiological metabolism activity of respiration duringgrowth of the Brassica campestris can be influenced by light intensity, so that the bud generation time of the Brassica campestris can be greatly delayed, under the premise that the bud generation time of the Brassica campestris can be greatly delayed, the yellowing rate of the Brassica campestris can be reduced, original appearance, shape and nutrient components of the Brassica campestris can bewell maintained. Through treatment with the plant hormones of a certain concentration, the bud generation and flowering of the Brassica campestris are restrained to a certain extent, and through cooperation with a storage manner with a low temperature freezer, the effect of restraining bud flowering after Brassica campestris is picked can be strengthened.

The invention relates to the technical field of agricultural production fertilizers, especially to a special water-soluble fertilizer for Brassica campestris, and a preparation method thereof, whereinthe raw materials comprise, by weight, 70-74 parts of ammonium sulfate, 15-17 parts of potassium chloride, 8-12 parts of urea, 4-6 parts of monoammonium phosphate, 14-17 parts of potassium fulvate, 3-7 parts of magnesium sulfate, 1-2 parts of a trace element amino acid chelate, 1-3 parts of a seaweed extract, 0.5-0.9 part of compound microorganisms, 0.2-0.6 part of gamma-polyglutamic acid and 0.2-0.6 part of borax. According to the invention, the special water-soluble fertilizer used for Brassica campestris and obtained through pre-mixing and raw material crushing has the reasonable ratio ofnitrogen to phosphorus to potassium to medium and trace nutrient elements according to the growth fertilizer requirement rule of Brassica campestris, can meet the nutrient requirements of Brassica campestris in the whole growth period, and can improve the soil fertility, improve the soil structure and solve the problems of soil acidification and hardening.

The invention provides a method for increasing embryoid emergence quantity in brassica chinensis isolated microspore culture. The method includes: step one, selecting a brassica chinensis bud in a late mononuclear stage, disinfecting, separating and purifying to obtain isolated microspores, suspending the purified microspores in an NLN liquid culture medium, and adjusting the microspore density to1*10<5>-2*10<5> microspores/mL by a blood counting chamber to obtain microspore suspension; step two, adding the prepared brassica chinensis microspores into a NLN-13 culture medium in which macroelements are reduced by half and 6-BA (0.05mg/L), NAA (0.10mg/L) and activated carbon (100mg/L) are added, subjecting sub-packaged microspore petri dishes to 32 DEG C heat shock treatment in a constant-temperature incubator for 24h, and then transferring into a 25 DEG C constant-temperature incubator for continuing dark culture until embryoids appear. By adoption of the method, an evident promoting effect on brassica chinensis microspore induced embryoid emergence is achieved, the microspore embryoid emergence quantity can be increased. The method has advantages of practicality, specific steps, high repeatability and the like, and extensive application of brassica chinensis isolated microspore culture to haploid breeding can be realized.

The invention discloses an evaluation method for heat resistance of flowering cabbages, and relates to the technical field of evaluation of heat resistance of vegetables. The method comprises the following steps: determining the optimal high-temperature stress germination temperature of flowering cabbages, and screening the optimal high-temperature stress germination temperature through the following indexes: relative germination rate, relative germination potential, relative germination index and relative vitality index; obtaining heat resistance evaluation index values of the flowering cabbages at the optimal high-temperature stress germination temperature, wherein the heat resistance evaluation index values comprise the relative germination rate, the relative germination potential, the relative germination index, the relative vitality index, the relative bud length, the relative bud thickness, the relative bud fresh weight, the relative bud dry weight, the relative root length, the relative root thickness, the relative root fresh weight and the relative root dry weight of bud seedlings; and calculating a grading evaluation value, a comprehensive grading value D and a grading coefficient GC of each flowering cabbage strain through the heat resistance evaluation index values. The method has the advantages of short identification period, high efficiency, low cost and the like, and is suitable for large-scale flowering cabbage strain evaluation and screening.

The invention belongs to the technical field of efficient and sustainable production in modern agriculture, and discloses a method for increasing yield of Chinese flowering cabbages by using a soil conditioner. The method comprises the following steps: cultivating Chinese flowering cabbage seedlings by using 72-hole trays and transplanting and planting the Chinese flowering cabbage seedlings into non-woven bags with 8-10 plants in each bag when the Chinese flowering cabbage seedlings have 3-4 true leaves; applying a three-element compound fertilizer N-P2O5-K2O:15-15-15 is applied to each cultivation bag and applying topdressing in three times in a seedling growing period, a leaf growing period and a forming period of Chinese flowering cabbages with the amount of the topdressing accounting for 25-35%, 30-40% and 30-40% of the total topdressing amount each time; and after transplanting, diluting a soil conditioner by 500-1500 times with clean water, such that yield of Chinese flowering cabbages is increased. Under a cultivation condition that the diluted soil conditioner is sprayed and the chemical fertilizer is applied, the yield and quality of Chinese flowering cabbages are improved, comprehensive characters of the soil are obviously improved, and continuous cropping obstacles are relieved.

The invention discloses a fresh-keeping system for internal and external separation treatment on flowering Chinese cabbages. The system comprises partition plates, a packaging system, an ozone water tank, an electric control box, a fresh-keeping box and a feeding groove, the packaging system and the ozone water tank are mounted on the fresh-keeping box, the ozone water tank is controlled by the electric control box, the feeding groove is formed in the fresh-keeping box, the feeding groove has a right triangle structure, an upper baffle plate is movable, the feeding groove further comprises a feeding port located in the fresh-keeping box, the two partition plates are located on the left side and the right side of the fresh-keeping box, a layering frame is directly matched with the feeding port, the feeding groove has a right triangle structure, vegetables entering from the feeding port slide onto the partition plate under the gravity action of the inclined surface, the Chinese floweringcabbages which are uniformly stored layer by layer by the partition plates can be prevented from being extruded due to stacking, and ozone water from a drainage tube is homogenized by round holes formed in each partition plate, so that the Chinese flowering cabbages on each partition plate can be treated by the ozone water, and the problem of large soaking uniformity difference can be solved.

The invention discloses a method for prevention and control of phyllotreta striolata of Chinese flowering cabbages by carpet type coverage with insect-proof nets. The method includes steps: 1) killingphyllotreta striolata eggs in soil, and sowing; 2) covering the ground with the insect-proof nets, and compacting all around with soil; 2) after coverage with the nets, spraying water and fertilizersto enable contact of the seeds with soil; 4) 5-15 days later, removing the nets, and eliminating adult phyllotreta striolata. By direct coverage of the ground with the insect-proof nets, greenhouse construction is avoided, and accordingly simplicity in operation, material saving and labor saving are realized; the insect-proof nets directly cover the ground and completely contact with the Chineseflowering cabbages and the ground, so that phyllotreta striolata cannot immigrate for spreading harms, and great prevention and treatment effects are achieved; by soil treatment before sowing, potential larvae and eggs of phyllotreta striolata in soil can be killed so as to eradicate hidden sources of injurious insects; low cost in prevention and control is realized.

The invention provides a lighting culture method capable of increasing size and promoting green for green terrier and brassica parachinensis bailey, and relates to planting and culturing of green terrier and brassica parachinensis bailey. In the seedling growth period of the green terrier and the brassica parachinensis bailey, an artificial light source is adopted for irradiating green terrier andbrassica parachinensis bailey seedlings, growth of leaves of the green terrier and brassica parachinensis bailey seedlings is facilitated, leaves change green, and chlorophyll is accumulated. A lightsupply manner of the artificial light sources adopts conventional white light and low-dosage UV-B lighting. The wavelength of low-dosage UV-B light is 280-320nm, and light quantum flux density is 3<mu>mol.m<-2>.s<-1>. The method disclosed by the invention is suitable for growth of total artificial light green terrier and brassica parachinensis bailey seedlings. The lighting method is adopted, sothat growth of the green terrier and brassica parachinensis bailey seedlings can be promoted, the production cycle can be shortened, and energy consumption is reduced. The method is simple and convenient to operate, and has wide application space and market prospects for agricultural modernization production.

The invention discloses a single cabbage seedling positioning device, and relates to the technical field of agricultural equipment. The single cabbage seedling positioning device comprises a supporting frame and a positioning device body, wherein the positioning device body is arranged on the supporting frame; the supporting frame is composed of a fixed plate, a fixed block, support rods and a sleeve pipe; the fixed plate is provided with the fixed block and the sleeve pipe; the fixed block is provided with the support rods; the positioning device body is composed of a connecting shaft, a connecting rod, a clamping block and a connecting block; the connecting shaft is connected with the sleeve pipe; the connecting shaft is provided with the connecting rod; the connecting rod is provided with the clamping block; the clamping block is provided with the connecting block. The single cabbage seedling positioning device is high in practicality and very convenient to use; indoor space can bemaximally used for planting Guangdong cabbage, and the Guangdong cabbage is positioned for growth, so that the yield and quality of the finished cabbage are improved, and the economic incomes of production enterprises or vegetable farmers are increased; and cutting is convenient, so that quick picking of the ripened Guangdong cabbage is facilitated and the burden of the production enterprises or the vegetable farmers is reduced.

The invention discloses a method for producing Chinese flowering cabbage hybrid seeds, and belongs to the technical field of crossbreeding. The method for producing the Chinese flowering cabbage hybrid seeds comprises the following steps that a Chinese flowering cabbage male parent is sown in two stages, the Chinese flowering cabbage male parent in the first stage and a Chinese flowering cabbage female parent are sown at the same time, and the Chinese flowering cabbage male parent in the second stage is sown after the Chinese flowering cabbage male parent in the first stage is sown for 10-12 days. According to the method for producing the Chinese flowering cabbage hybrid seeds, the flowering phases of the male parent and the female parent of a Chinese flowering cabbage are relatively concentrated, concentrated pollination is facilitated, meanwhile, the number of bent burst and development atrophy of fruit pods is effectively reduced, complete development of the Chinese flowering cabbage seeds is effectively promoted, the quality and the germination rate of the Chinese flowering cabbage hybrid seeds are further improved, and a foundation is provided for planting and popularization of the Chinese flowering cabbage.

The invention discloses a cultivation method for planting flowering cabbage in a greenhouse. The cultivation method comprises the first step of garden plot selection and land preparation; the second step of soil treatment before sowing, wherein deep plowing is conducted to make high ridges, the ground is made flat, soil particles are smashed, base fertilizer is fully applied, and the distance between the ridges is 0.8-1.2 m; the third step of seed treatment, wherein seeds are soaked with warm water, washed and then put at a warm place for accelerating germination, and sowing is conducted after the sprouts come out; the fourth step of seedling emergence management, wherein watering is conducted after sowing, intense watering is not allowed, and sprinkling irrigation must be conducted and not stopped until soil is wet; the fifth step of planting management; the sixth step of flowering cabbage watering, wherein during water sprinkling, water drops are uniform, and fine water is sprinkled on ridge surfaces and leaf surfaces in a spray mode; the seventh step of flowering cabbage harvesting, wherein the flowering cabbage is harvested in the morning. The cultivation method is simple, easy to implement and low in cost; through reasonable use of fertilizer and water and scientific growing of seedlings, sufficient nutrition is provided for the flowering cabbage, and the growing environment of the flowering cabbage is optimized. The flowering cabbage cultivated through the method is uniform in size and does not have yellow flowers or yellow leaves or bad leaves, and the yield is as high as 3,000 jin or above.

The invention provides a sliding-type daylighting adjusting device for brassica parachinensis bailey planting and relates to the technical field of agricultural production equipment. The sliding-typedaylighting adjusting device comprises a first connecting rod, support rods, a second connecting rod, a first transmission rod, a second transmission rod, transmission devices and a daylighting adjusting device, wherein the support rods are arranged on the first connecting rod; the first transmission rod and the second transmission rod are arranged on the second connecting rod; one transmission device is arranged in each of the first transmission rod and the second transmission rod; each transmission device comprises an upper belt pulley, a lower belt pulley, a transmission belt, a fixing groove, a connecting shaft and a handle; and the daylighting adjusting device is arranged between the first connecting rod and the second connecting rod and comprises a first reel, a second reel, fixing rings, black-out cloth and a daylight opening. The sliding-type daylighting adjusting device is high in practicability and very convenient to use, not only can adjust daylighting of Guangdong brassicaparachinensis bailey when the light is too strong, but also can assist positioning of the Guangdong brassica parachinensis bailey, greatly ensures normal growth of the Guangdong brassica parachinensisbailey and improves the planting production benefit of a planter.

The invention belongs to the technical field of vegetable preservation, and particularly relates to a preservation packaging method for late flowering Chinese cabbages. The preservation packaging method comprises the following steps: 1) carrying out pretreatment, specifically, picking the late flowering Chinese cabbages, cleaning the late flowering Chinese cabbages, disinfecting the stem base parts and carrying out precooling; and 2) carrying out preservation packaging treatment, specifically, packaging the late flowering Chinese cabbages by adopting low-density polyethylene functional air-conditioning bags, carrying out LED lamp illumination and then placing the late flowering Chinese cabbages in a foam box. The low-density polyethylene functional air-conditioning bags are made of low-density polyethylene LDPE, and the LED lamp illumination adopts red and blue light combined illumination; a mild and effective sterilization solution is adopted, and in combination with low temperature and the method adopting the air-conditioning bags, quality deterioration such as leaf moisture evaporation, water loss withering, flower bud blooming and hollow stem part can be remarkably prevented; and in combination with the red and blue light combination, the clean late flowering Chinese cabbages are properly disinfected and sterilized, so that the activity of microorganisms is inhibited, excessive water vapor evaporation can be reduced, the operation method is simple, effective, safe and environment-friendly, and preservation is facilitated in the normal-temperature transportation process.

The invention relates to the application field of cell engineering, in particular to a high-frequency Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee in-vitro tissue culture method. According to the method, seeds of Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee are subjected to surface disinfection and then placed on a sowing culture medium for culture to obtain sterile seedlings with a seedling age of 3-5 d, then cotyledons with petiole are stably cut above growth points as explants, and the explants are vertically inserted into a differentiation culture medium for differentiation culture to obtain adventitious buds; the adventitious buds are transferred to a rooting culture medium for rooting culture to obtain tissue culture seedlings; and the tissue cultureseedlings are subjected to bottle opening and seedling hardening, and the follow-up routine management is performed. The Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee high-frequency regeneration system established by the invention has high adventitious bud regeneration rate, easy formation of callus, low pollution rate, simple and convenient operation, and high comprehensive performance, and a foundation for the establishment of a Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee genetic transformation system is laid.

The invention discloses an all artificial light control method of leaf vegetable forms. The ratio of the number of photons with the wavelength of 710-740 nm to the number of photons with the wavelength of 400-499 nm in a light environment is 0.87-1.10; the wavelength range of the light environment is 380-780 nm; the proportion of the number of photons with the wavelength of 710-740 nm is 9.3-14.5%; and the specific spectral composition is as follows: the proportion of the number of photons with the wavelength of 400-499 nm is 10-15%; the proportion of the number of photons with the wavelengthof 500-599 nm is 21-27%; the proportion of the number of photons with the wavelength of 600-699 nm is 37-54%; and the proportion of the number of photons with the wavelength of 700-780 nm is 12-22%. In an environment that other growth conditions are kept and the light intensity is same, brassicaceous vegetables, which are upright in plant type, better in waist binding, relatively tight in cabbagecore compactness and relatively compact in whole plant form can be bred in the light environment; and thus, the commodity of leaf vegetables is improved.

Organic fertilizer special for Chinese flowering cabbage is prepared from 50 to 90 parts of mushroom dreg, 50 to 90 parts of peanut bran, 50 to 90 parts of chicken manure, 50 to 90 parts of bean pulp,straw, 15 to 30 parts of monosodium phosphate, 3 to 5 parts of borax, 3 to 5 parts of sodium selenate and 3 to 5 parts of EDTA (Ehtylene Diamine Tetraacetic Acid) iron chelate. The problem that continuous cropping of Chinese flowering cabbage cannot be carried out can be solved, or the problem of low output caused by continuous cropping can be solved.

The invention provides a Chinese flowering cabbage seed coating agent and a use method thereof, and belongs to the technical field of seed coating. The Chinese flowering cabbage seed coating agent is prepared from the following raw materials: dinotefuran insecticide, an emulsifier, acetonitrile, water, a filler and an adhesive. The seed coating agent is used for coating Chinese flowering cabbage seeds, the disease resistance of the seeds can be improved, dinotefuran is selected as a seed pellet insecticidal active substance, the seeds can go into the field with pesticide and be accurately applied, the good control effect on underground pests is achieved, meanwhile, the growth of plants in the seedling stage can be effectively protected, the loss caused by pests in the vegetable seedling stage is effectively reduced, and the pesticide cost can be greatly reduced by using the dinotefuran technical.

The invention relates to a flowering cabbage cutting device with a fresh-keeping function, and relates to the technical field of agricultural equipment. The flowering cabbage cutting device comprisesa first cutting board, a heat conduction pipe, a sleeve, a pressure relief valve, a second cutting board, a grip and a sealing plug, the heat conduction pipe, the pressure relief valve, the second cutting board and the grip are arranged on the first cutting board, the sleeve is arranged on the heat conduction pipe, the sealing plug is arranged on the grip, the first cutting board is formed by a first fixing rod, a second fixing rod, a first connecting rod and a second connecting rod, and the second cutting board is composed of a first connecting plate, a second connecting plate and a connecting block. The flowering cabbage cutting device is high in practicability and very convenient to use, harvested Guangdong flowering cabbages are quickly frozen and preserved, the freshness of the flowering cabbages is improved, later marketing sales of the flowering cabbages is facilitated, the economic income of production enterprises or vegetable growers is increased, cutting and preservation arecompleted at a time, the marketing sales time of the flowering cabbages is saved, the labor intensity of workers is reduced, the production efficiency is improved, and the cost of the production enterprises or the vegetable growers is saved.