A long-term multimodal information detection method and system for in situ culture of living cells

Abstract

The invention relates to a long-term multi-mode information detection method and system for in-situ culture of living cells. The detection system includes a microfluidic chip in-situ culture module for living cells, a multi-mode information detection module, a multi-dimensional motion control module and digital information storage. The processing and display module; the microfluidic chip living cell in situ culture module is used for culturing living cells; the multimodal information detection module adopts white light interference hyperspectral analysis, absorption or emission spectrum analysis, electrical impedance, cell autofluorescence and / or Long-term in situ non-destructive detection of living cells by means of excited fluorescence; the digital information storage processing and display module controls the movement of living cells in the in situ culture module of microfluidic chip living cells through the multi-dimensional motion control module to obtain the three-dimensional space of living cells The location information and detection information are stored, processed and displayed, and the long-term, in-situ and five-dimensional information fusion detection of multi-mode information of living cells is completed.

Description

technical field [0001] The invention relates to a long-term multi-mode information detection method and system for in situ culture of living cells, and relates to the technical field of cell analysis and detection. Background technique [0002] As the basic unit of life activities, cells are very important in basic scientific research and clinical applications of life sciences and medicine. Based on CO 2 Constant temperature incubator, microscopic technology and detection methods of cell morphology, structure and function of fluorescent markers have been unable to satisfy people's needs for living cell growth and development, migration, division and differentiation, apoptosis and carcinogenesis, immune tolerance and response of cells, cell Requirements for the analysis of complex cell behavior and functions such as phagocytosis, interactions between cells, cells and substrates, and interactions between cells and soluble molecules. High-resolution measurement methods such as...

AI Technical Summary

Problems solved by technology

For the detection of living cells, especially for the detection of suspension cells, it is difficult to design cell culture devices and build an online detection system with multi-modal fusion under the space constraints of such a short working distance

The confocal scanning method of optical fluorescent labeling can realize the detection of cell slices at the subcellular level, but its speed is slow, and fluorescent labeling often affects cell viability. Practical application needs of information in situ detection

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