Living cell in-situ culture long-time-course multimode information detecting method and system
A technology of information detection and in situ culture, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve the problem of slow speed, failure to meet application needs, and difficulty in designing multi-mode cell culture devices Converged online detection system and other issues
Active Publication Date: 2018-11-30
TSINGHUA UNIV
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Problems solved by technology
For the detection of living cells, especially for the detection of suspension cells, it is difficult to design cell culture devices and build an online detection system with multi-modal fusion under the space constraints of such a short working distance
The confocal scanning method of optical fluorescent labeling can realize the detection of cell slices at the subcellular level, but its speed is slow, and fluorescent labeling often affects cell viability. Practical application needs of information in situ detection
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[0027] The present invention will be described in detail below in conjunction with the accompanying drawings. However, it should be understood that the accompanying drawings are provided only for better understanding of the present invention, and they should not be construed as limiting the present invention.
[0028] Such as figure 1 As shown, the long-term multimodal information detection system for live cell in situ culture provided by the present invention includes a microfluidic chip live cell in situ culture module MC, a multimode information detection module MO, a multidimensional motion control module MD and digital information storage and processing with display module SP.
[0029] The microfluidic chip living cell in situ culture module MC is used for culturing living cells, cultivating and maintaining the growth and activity state of living cells.
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The invention relates to a living cell in-situ culture long-time-course multimode information detecting method and system. The living cell in-situ culture long-time-course multimode information detecting system comprises a micro-fluidic chip living cell in-situ culture module, a multimode information detection module, a multidimensional movement control module and a digital information storage processing and display module. The micro-fluidic chip living cell in-situ culture module is used for culturing living cells. The multimode information detection module conducts long-time-course in-situ nondestructive detection on the living cells in the modes of high spectral analysis interference through white light, spectral analysis absorption or emission, electrical impedance, cell spontaneous fluorescence and / or fluorescence excitation. The digital information storage processing and display module controls movement of the living cells in the micro-fluidic chip living cell in-situ culture module through the multidimensional movement control module, and obtains three-dimensional spatial position information and detection information of the living cells to be stored, processed and displayed, and long-time-course, in-situ and five-dimensional information fusion detection of living cell multimode information is completed.
Description
technical field [0001] The invention relates to a long-term multi-mode information detection method and system for in situ culture of living cells, and relates to the technical field of cell analysis and detection. Background technique [0002] As the basic unit of life activities, cells are very important in basic scientific research and clinical applications of life sciences and medicine. Based on CO 2 Constant temperature incubator, microscopic technology and detection methods of cell morphology, structure and function of fluorescent markers have been unable to satisfy people's needs for living cell growth and development, migration, division and differentiation, apoptosis and carcinogenesis, immune tolerance and response of cells, cell Requirements for the analysis of complex cell behavior and functions such as phagocytosis, interactions between cells, cells and substrates, and interactions between cells and soluble molecules. High-resolution measurement methods such as...
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IPC IPC(8): C12M3/00C12M1/38C12M1/36C12M1/34
CPCC12M23/16C12M41/00C12M41/12C12M41/34C12M41/48
Inventor 黄国亮符荣鑫苏雅
Owner TSINGHUA UNIV