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A Method for Evaluating Water Quality Using Single Cell Metabolic Rate Index

A technology of cell metabolism and rate, applied in biochemical equipment and methods, test water, microbial measurement/inspection, etc., can solve problems such as evaluating water pollution levels

Active Publication Date: 2021-03-02
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these indicators fail to accurately and comprehensively evaluate the level of water pollution, especially the joint effects of nutrient levels and xenobiotics.

Method used

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  • A Method for Evaluating Water Quality Using Single Cell Metabolic Rate Index
  • A Method for Evaluating Water Quality Using Single Cell Metabolic Rate Index
  • A Method for Evaluating Water Quality Using Single Cell Metabolic Rate Index

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, sample collection used in the present invention and water quality determination method

[0034] 1.1 Sample collection

[0035] The Chaobai River (39° to 40° north latitude, 116° to 117° east longitude) basin in the Haihe River system in China was selected as the research object. Sampling was carried out in the Chaobai River Basin in June 2017 (summer). Referring to the previous research on the Chaobai River Basin, a total of 22 sampling points were selected. The sampling points were all located in the main channel of the Chaobai River and the inlets of important tributaries. They were 5 high mountain areas, 6 urban areas and 11 farmland areas . There are 25 samples in total.

[0036] 1.2 Physicochemical analysis of samples

[0037] The analysis methods of the basic physical and chemical indicators of Chaobai River water quality mainly refer to the national standards of the People's Republic of China and "Water and Wastewater Monitoring and Analysis Met...

Embodiment 2

[0050] Embodiment 2 uses single cell metabolic rate index (natural organic matter metabolic index) to evaluate water quality

[0051] 2.1 Physical and chemical indicators of river water in alpine areas, urban areas and farmland areas

[0052] As described in embodiment 1, carried out summer sampling, summer (June) average water temperature is respectively 28.6 ℃, and water quality situation schematic diagram is shown in figure 1 . Overall, the concentrations of TN, TP and DOC in alpine areas were lower than those in urban areas and farmland areas. The concentration of DOC in the urban area was significantly higher than that in the other two areas, and the concentration of TP in the farmland area was higher.

[0053] 2.2 Microbial biomass in alpine areas, urban areas and farmland areas

[0054] From figure 2 From the results of medium biomass, there was extremely significant difference in biomass (p<0.001). Due to the lower nutrient levels in alpine areas, their biomass i...

Embodiment 3

[0074] Embodiment 3 uses single cell metabolic rate index (natural organic matter metabolic index) to evaluate water quality

[0075] The accuracy and universality of the proposed threshold of natural organic matter metabolic index are verified by Example 2, and the single cell metabolic rate index (natural organic matter metabolic index) is applied to the water quality evaluation of actual water bodies. 3.1 Physical and chemical indicators of river water in alpine areas, urban areas and farmland areas

[0076] In September 2017, the same sampling point in Example 2 was sampled again for water quality sample analysis. Among them, the concentration limits of nitrogen and phosphorus in urban areas and farmland areas are beyond the scope of Class V water. The water quality in high mountainous areas is better, while the water quality in urban areas and farmland areas is affected by human activities and is poor. For a diagram of water quality, see Figure 6 , nitrogen and phosph...

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Abstract

The invention belongs to the technical field of sewage treatment, and in particular relates to a method for evaluating water quality by using a single cell metabolic rate index. This method by Biolog EcoPlate TM Detect the metabolic characteristics of microorganisms in the river ecosystem, use flow cytometry to detect the biomass of water samples, and fit the above data into a single cell metabolic rate index (natural organic matter metabolic index), so that the index reflects the nutrient level and xenobiotics. Together, to achieve the effect of indicating changes in water quality.

Description

Technical field: [0001] The invention belongs to the technical field of sewage treatment, and in particular relates to a method for evaluating water quality by using a single cell metabolic rate index. Background technique: [0002] The traditional water quality evaluation is mainly based on physical and chemical indicators including the concentration of various pollutants. Through qualitative or quantitative analysis of these indicators, the pollution status of the river system is evaluated. The concentration of some or a certain type of pollutants, and the monitoring results are very accurate, but it cannot accurately distinguish the total pollution effect of different types of pollutants, nor can it directly reflect the impact of various types of exogenous pollution input on the organisms in the ecosystem . [0003] Because microorganisms can respond quickly and sensitively to external environmental pressure, researchers have long been committed to exploring the feasibil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/06G01N21/31G01N33/18
CPCC12Q1/06G01N21/31G01N33/18
Inventor 柏耀辉廖恺玲俐菅之舆赵琛曲久辉
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI