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Streptomycete for producing chitinase and application thereof

A technology of chitinase and streptomyces, applied in the direction of application, bacteria, and microorganism-based methods, can solve the problems of hidden dangers of pesticide residues and achieve the effect of not producing drug resistance

Active Publication Date: 2018-12-07
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of hidden dangers of pesticide residues in the existing methods for controlling soybean gray mold and black spot, and provide a strain of streptomyces producing chitinase and its application

Method used

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  • Streptomycete for producing chitinase and application thereof
  • Streptomycete for producing chitinase and application thereof
  • Streptomycete for producing chitinase and application thereof

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specific Embodiment approach 1

[0021] Embodiment 1: A chitinase-producing Streptomyces strain of the present embodiment is Streptomycess p. G6, which is preserved in the General Microorganism Center of the China Microorganism Culture Collection Administration Committee, and the preservation address is Beichen West, Chaoyang District, Beijing. No. 3, Courtyard No. 1, Road 1, the preservation date is February 2, 2018, and the preservation number is CGMCCNo.15334.

[0022] This embodiment adopts wild rice rhizosphere soil to isolate and purify chitinase-producing strains:

[0023] Preliminary screening: Using the plate dilution coating method, weigh 5 g of rhizosphere soil samples from Dongxiang wild rice in Jiangxi into 45 ml of sterile water, stand at 120 rpm for 20 min, and take the supernatant for 10-fold concentration gradient dilution. Take 10 respectively -1 , 10 -2 , 10 -3 Each concentration of the dilution solution was 80 μL, and each gradient was spread on 3 chitin medium plates, and sterile water...

specific Embodiment approach 2

[0027] Specific embodiment 2: The application of a chitinase-producing Streptomyces strain in this embodiment refers to the application in the prevention and control of soybean diseases.

[0028] This embodiment provides a Streptomyces G6 strain screened in the rhizosphere soil of wild rice, which has chitinase activity and has an inhibitory effect on soybean botrytis and black spot. The preservation number is CGMCC NO.15334, which is in In the rhizosphere soil of wild rice in different locations in Dongxiang, Jiangxi Province, strains with strong ability to decompose chitin were obtained after primary selection, and then the strains with good antagonistic effect against soybean botrytis and black spot were repeatedly screened. After a large number of experiments It is proved that strain G6 not only has chitinase activity, but also has a good antagonistic effect on soybean gray mold and black spot. The inhibitory effect of G6 fermentation broth on gray mold reaches 91.7%, and t...

specific Embodiment approach 3

[0029] Embodiment 3: The difference between this embodiment and Embodiment 2 is that the chitinase-producing Streptomyces sp. is used in the inhibition of black spot disease. Others are the same as in the second embodiment.

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Abstract

The invention relates to a streptomycete for producing chitinase and an application thereof. An object of the invention is to solve the problem that conventional methods for controlling soybean gray mold and black spot diseases have hidden danger of pesticide residues. The streptomycete for producing the chitinase is streptomycete (Streptomyces sp.) G6, and is preserved in a general microbiology center of China Committee for Culture Collection of Microorganisms, and a preservation address is No.3, No.1 yard, Beichen west road, Chaoyang District, Beijing, a preservation date is February 02, 2018, and a preservation number is CGMCC No.15334. The application of the streptomycete for producing the chitinase refers to the application of prevention and control of soybean diseases. The bacterialstrain G6 not only has chitinase activity, but also has a good antagonistic effect on the soybean gray mold and black spot diseases, is free of the pesticide residues, and does not produce drug resistance. The streptomycete for producing the chitinase and the application thereof are applied to the technical field of environmental microorganisms.

Description

technical field [0001] The invention relates to a chitinase-producing Streptomyces strain and its application. Background technique [0002] Soybean is the main food crop in my country, as well as the main cash crop and feed crop. With the continuous growth of the population and the improvement and promotion of living standards, the yield and quality of soybeans are the focus of attention. Botrytis cinerea and black spot disease of soybean will infect the whole growth period of soybean, thus causing soybean disease, which are distributed in Jilin, Liaoning, Heilongjiang, Jiangsu, Sichuan and other provinces. From the existing reports, it is known that soybean gray mold and black spot not only infect soybean, but also have pathogenicity on ginseng, citrus, sweet potato, cabbage, jujube fruit, rose and other plants. [0003] At present, the main method to control soybean gray mold and black spot is still mainly chemical pesticides, but in view of the hidden dangers of pestic...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A61P3/00C12R1/465
CPCA01N63/10C12N1/205C12R2001/465
Inventor 田春杰田磊吉丽谢金宏陈德国张嘉旭张建峰马丽娜石少华罗莎莎常晶晶常春玲徐尚起
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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