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Preparation method of galacto-oligosaccharide

A technology of galactooligosaccharides and galactosidase, which is applied in the field of preparation of galactooligosaccharides, can solve problems such as loss and blockage of reaction columns, achieve good flexibility, stable physical and chemical properties, and solve the problems of loss and blockage of reaction columns problem effect

Active Publication Date: 2018-12-07
量子高科(广东)生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The polyvinyl chloride-silicon dioxide film novel organic-inorganic composite material used in the present invention has good flexibility, and the membrane enzyme reactor formed after fixing the β-galactosidase can react under agitation, and can also Rolled into a column and packed in a cylindrical container, allowing the substrate lactose to react when it passes through, effectively solving the problem of loss and clogging of the reaction column during the recovery process of small granular immobilized enzymes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A preparation method of galactooligosaccharides, comprising the following steps:

[0027] (1) Put the polyvinyl chloride-silicon dioxide film 600cm 2 (about 30g) winding, forms the membrane reactor of cylindrical shape;

[0028] (2) Take 230 mL of β-galactosidase enzyme solution with an enzyme activity of 80 u / mL that has been purified by salting out, and circulate it with the membrane reactor obtained in step (1) at 20-25°C for 20 hours to obtain immobilized β-galactosidase Enzyme membrane reactor for lactosidase;

[0029] (3) The lactose solution that 1800mL concentration is 52% (w / w) is transported in the enzyme membrane reactor, circulates 13h at 45-52 ℃, obtains the galacto-oligosaccharide that 1650mL concentration is 55% (w / w) The syrup, wherein the monosaccharide content is 24.02%, the lactose content is 14.98%, and the galacto-oligosaccharide content is 61.00%.

Embodiment 2

[0031] A preparation method of galactooligosaccharides, comprising the following steps:

[0032] (1) Put the polyvinyl chloride-silicon dioxide film 750cm 2 (about 38g) winding, forms the membrane reactor of cylindrical shape;

[0033] (2) Take 300 mL of β-galactosidase enzyme solution with an enzyme activity of 75u / mL that has been purified by salting out, and react with the membrane reactor obtained in step (1) at 15-20°C for 18 hours to obtain immobilized β-galactosidase. Enzyme membrane reactor for galactosidase;

[0034] (3) 2300mL concentration of 48% (w / w) of lactose solution was delivered to the enzyme membrane reactor, and circulated for 16h at 45-52°C to obtain 2100mL concentration of 50% (w / w) galactooligosaccharides Syrup, wherein the monosaccharide content is 25.88%, the lactose content is 13.48%, and the galacto-oligosaccharide content is 60.64%;

[0035] (4) Dilute the galacto-oligosaccharide syrup to 20% (w / w), and then use a nanofiltration membrane with a m...

Embodiment 3

[0037] A preparation method of galactooligosaccharides, comprising the following steps:

[0038] (1) The polyvinyl chloride-silicon dioxide film 2.0m 2 (about 1000g) winding, forms the membrane reactor of cylindrical shape;

[0039] (2) Take 9000 mL of β-galactosidase enzyme solution with an enzyme activity of 68u / mL that has been purified by salting out, and circulate it with the membrane reactor obtained in step (1) at 10-15°C for 24 hours to obtain immobilized β-galactosidase Enzyme membrane reactor for lactosidase;

[0040] (3) 50L of lactose solution with a concentration of 49% (w / w) was delivered to the enzyme membrane reactor, and circulated for 24h at 45-52°C to obtain 47L of galactooligosaccharides with a concentration of 50% (w / w). Syrup, wherein the monosaccharide content is 28.87%, the lactose content is 11.11%, and the galacto-oligosaccharide content is 60.02%.

[0041] (4) Dilute the galacto-oligosaccharide syrup to 20% (w / w), then use the nanofiltration membr...

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Abstract

The invention belongs to the technical field of functional oligosaccharide preparation, and concretely relates to a preparation method of a galacto-oligosaccharide. A novel organic-inorganic compositematerial of a polyvinyl chloride-silicon dioxide film used in the invention has good flexibility. A membrane enzyme reactor formed by immobilizing beta-galactosidase can react in a stirring state orbe rolled up into a column to be packed in a cylindrical container so as to enable substrate lactose to react when passing through the reactor, which effectively solves the problems of loss and reaction column blockage in a recovery process of a small granular immobilization enzyme. According to the preparation method, the content of the galacto-oligosaccharide in primary conversion syrup is morethan 60% which is 24-57% higher than the content of the galacto-oligosaccharide in the primary conversion syrup by a conventional enzyme method. According to the preparation method, the content of thelactose in the primary conversion syrup is less than 15% which is 20-35% much lower than the content of the lactose in the primary conversion syrup by the conventional enzyme method.

Description

technical field [0001] The invention belongs to the technical field of preparation of functional oligosaccharides, and in particular relates to a preparation method of galacto-oligosaccharides. Background technique [0002] Galacto-oligosaccharides (GOS for short) is a functional oligosaccharide, which has the characteristics of good solubility, low cariogenicity, low calorific value and low sweetness value. Galacto-oligosaccharides can promote the growth of beneficial bacteria in the human intestinal tract, inhibit the growth of intestinal spoilage bacteria, improve lipid metabolism, reduce serum total cholesterol concentration, promote the absorption of mineral elements, reduce and prevent the occurrence of constipation, and reduce the accumulation of toxic substances in the colon. Content, promote intestinal health and other effects. [0003] Galactooligosaccharides are prepared from lactose by catalysis of β-galactosidase (EC3.2.1.23), and are obtained by β(1→2) bond or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/00C12P19/04C12P19/12
CPCC12P19/00C12P19/04C12P19/12C12P19/14
Inventor 陈子健伍艳梅谢志龙陈振鹏冯仲笑刘耿生
Owner 量子高科(广东)生物有限公司
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