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A kind of preparation method of soybean multifunctional hypoglycemic peptide

A multi-functional, soybean technology, applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of complex preparation process, single research on chemical components of soybean extracts, and low product solubility, and achieve a simple preparation process. , Enriching the scope of research, the effect of mild operating conditions

Active Publication Date: 2022-03-01
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The essential amino acids contained in the active peptide obtained by hydrolyzing soybeans are exactly the same as those of soybean protein, and the content is rich, which is more suitable for the digestion and absorption mechanism of the human body; compared with soybean protein, the advantages of various physical, chemical and physiological properties of active peptides are more prominent; The research on the chemical composition of soybean extract is relatively simple, the preparation process is complicated, and the solubility of the product is low

Method used

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  • A kind of preparation method of soybean multifunctional hypoglycemic peptide
  • A kind of preparation method of soybean multifunctional hypoglycemic peptide
  • A kind of preparation method of soybean multifunctional hypoglycemic peptide

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specific Embodiment approach 1

[0026] DETAILED DESCRIPTION OF THE INVENTION This embodiment discloses a method of preparing soy multifunctional dehydrate peptide, including the following steps:

[0027] Step 1: In addition to mixing soybeans, get soy flour, extract soy separation protein by alkaline acidic acid;

[0028] Step 2: Solting the soybean separation protein is prepared into a solution having a concentration of 2-5% (w / v, mass / volume), stir well, boiling water bath heat insulation 10-15 min, cool to room temperature, to obtain soy separation Protein solution;

[0029] Step 3: Adjusting the pH of the soy separation protein solution is 9.0-11.0, the temperature is 40-60 ° C, add a certain amount of alkaline protease, so that E / S is 6000 u / g, the enzymatic solution is 6-10 h;

[0030] Step 4: During the hydrolysis of the protein, 0.5 mol / L of the NaOH solution is added dropwise to the reaction system at different times, and the pH is constant at the initial pH, and the pH is constant, that is, t...

specific Embodiment approach 2

[0031] DETAILED DESCRIPTION OF THE INVENTION 2: The present embodiment discloses a method of preparing soy multifunctional destallous peptides, including the following steps:

[0032] Step 1: In addition to mixing soybeans, get soy flour, extract soy separation protein by alkaline acidic acid;

[0033] Step 2: Solting the soybean separation protein is prepared into a solution having a concentration of 2-5% (w / v, mass / volume), stir well, boiling water bath heat insulation 10-15 min, cool to room temperature, to obtain soy separation Protein solution;

[0034] Step 3: Adjusting the pH of the soy separation protein solution is 6.0-8.0, the temperature is 40-60 ° C, add a certain amount of trypsin, so that E / S is 6000 u / g, the enzymatic solution is 6-10 h;

[0035] Step 4: During the hydrolysis of the protein, 0.5 mol / L of the NaOH solution is added dropwise to the reaction system at different times, and the pH is constant at the initial pH, and the pH is constant, that is, ...

specific Embodiment approach 3

[0036] DETAILED DESCRIPTION OF THE INVENTION 3: The present embodiment discloses a method of preparing a multi-functional derivative peptide peptide, including the following steps:

[0037] Step 1: In addition to mixing soybeans, get soy flour, extract soy separation protein by alkaline acidic acid;

[0038] Step 2: Solting the soybean separation protein is prepared into a solution having a concentration of 2-5% (w / v, mass / volume), stir well, boiling water bath heat insulation 10-15 min, cool to room temperature, to obtain soy separation Protein solution;

[0039] Step 3: Adjusting the pH of the soy separation protein solution is 6.0-9.0, the temperature is 50-70 ° C, add a certain amount of papain protease, so that E / S is 6000 u / g, the enzymatic solution is 6-10 h;

[0040] Step 4: During the hydrolysis of the protein, 0.5 mol / L of the NaOH solution is added dropwise to the reaction system at different times, and the pH is constant at the initial pH, and the pH is const...

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Abstract

In order to expand the scope of application of soybeans and enhance their value for both medicine and food, the present invention records a method for preparing soybean multifunctional hypoglycemic peptides, which includes the following steps: removing impurities from soybeans and grinding them into powder, and extracting soybean protein isolates; Soybean protein isolate is formulated with deionized water into a solution with a concentration of 2-5% (w / v), stirred evenly, kept in a boiling water bath for 10-15min, and cooled to room temperature; pH and optimum temperature, add proteolytic enzyme to make E / S 6000U / g. During the process of protein hydrolysis, add NaOH solution dropwise to keep the pH value constant at the initial pH value until the end of the reaction, and record the volume of NaOH consumed to calculate the degree of protein hydrolysis; Heat in boiling water for 8-20min to inactivate the enzyme, then cool to room temperature, centrifuge to collect the supernatant, and freeze-dry to obtain soybean multifunctional hypoglycemic peptide. The invention belongs to the field of polypeptide preparation and has the functions of lowering blood sugar and blood pressure and antioxidative activity.

Description

Technical field [0001] The present invention belongs to the preparation of polypeptide, and in particular, it relates to a method of preparing soy multifunctional derivation peptide. Background technique [0002] Diabetes is called a healthy killer, causing a big threat to the human body. At present, it can only be treated with diabetes, failed to cure, long-term high blood sugar in diabetic patients will cause a series of complications, chronic cardiovascular disease, chronic renal failure and retinal injury, etc. According to statistics, 73.6% of diabetic patients suffer from hypertension, and the oxidation of the body has promoted the deterioration of the condition of diabetic patients. [0003] Studies have shown that hyperglycemia can be caused by suppressing glucose absorbed enzymes (α-glucosidase and DPP-IV enzyme) produced by inhibiting glucose (alpha-glucosidase and DPP-IV). The enzyme inhibition is derived from a biological reaction, and thus the target is gathered in a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/30
CPCC12P21/06C07K1/145C07K1/30
Inventor 王荣春董宇婷张英春程翠林何胜华
Owner HARBIN INST OF TECH
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