Method for detecting residual cells in single donor platelet product

Abstract

The invention relates to a method for detecting residual cells in a single donor platelet product. The method comprises the following steps: (1) extracting a cell sample, and adding a staining antibody regent, so as to finish specific labeling of the cell; (2) horizontally spreading the cell sample, carrying out observation and counting through a fluorescence microscope, wherein the staining antibody regent contains Alexa Fluor(R)488 labeled CD41 and CD61 for realizing the staining of blood platelets, Alexa Fluor(R)594 labeled CD45 for realizing the staining of leukocytes and a cell nucleus dye for realizing the staining of leukocyte nucleuses. According to the scheme, by utilizing a measure for combining cell dye labeling with fluorescence imaging, the simultaneous detection of residual leukocytes, erythrocytes and blood platelets can be realized; and the method is applicable to the fluorescence microscopes and software calculation so as to realize automatic detection and counting; and the method is simple in operation and high in efficiency.

Description

technical field [0001] The invention relates to the detection field of platelet products, in particular to a method for detecting residual cells in apheresis platelet products. Background technique [0002] At present, platelet blood transfusion is one of the most important treatment methods for thrombocytopenia and various diseases, and has developed into the second most clinical component blood transfusion after red blood cell transfusion. However, ineffective platelet transfusions still occur frequently. Blood patients generally need multiple platelet transfusions. Ineffective transfusions are likely to occur after multiple transfusions of platelets. The main reason is the production of allotype antibodies. Studies have shown that, HLA antibody, HPA antibody and ABH antibody exist in most patients with ineffective platelet transfusion, and 80% of them have HLA antibody. The main reason for the antibody production is likely to be the stimulation of the patient's immune sy...

AI Technical Summary

Problems solved by technology

In recent years, although some blood banks have adopted more accurate flow cytometry analysis, the relative sample preparation of flow cytometry is cumbersome, has high requirements for operators, and is expensive, so it is not universally applicable

[0006] The current residual cell detection method has many problems and limitations

The manual counting method based on the large-capacity Nageotte blood cell counting disc has large errors, poor repeatability, and no original records, which do not meet modern requirements for record keeping and chain of evidence

[0007] Conventional blood cell counters on the market are difficult to accurately count relevant indicators in apheresis platelet products, especially residual white blood cells and red blood cells

The detection limit of commonly used cell counting methods (such as blood cell counter, flow cytometer, etc.) is generally at 10 4 cells / mL, it is impossible to quickly and effectively detect the residual cells in the whitened apheresis platelets

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