Method for detecting residual cells in single donor platelet product

A detection method, platelet technology, applied in the direction of measuring devices, individual particle analysis, scientific instruments, etc., can solve the problems of large limitations, large errors, high prices, etc., achieve high efficiency, realize automatic detection and counting, and simple operation Effect

A detection method, platelet technology, applied in the direction of measuring devices, individual particle analysis, scientific instruments, etc., can solve the problems of large limitations, large errors, high prices, etc., achieve high efficiency, realize automatic detection and counting, and simple operation Effect

CN108956431AActive Publication Date: 2018-12-07苏州叠代生物科技有限公司
10 Cites 0 Cited by

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting residual cells in single donor platelet product
  • Method for detecting residual cells in single donor platelet product
  • Method for detecting residual cells in single donor platelet product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0036] 1. Materials

[0037] 1. Cell samples;

[0038] 2. Fluorescent dyes:

[0039] CD41 (GeneTex, Cat. No. GTX113758), CD61 (Wuhan Boout, Cat. No. orb86360), both CD41 and 61 were detected by Alexa 488 (ThermoFisher Company) mark;

[0040] CD45 (Shanghai Yanhui, Cat. No. 11-0451-82), with Alexa 594 (ThermoFisher Company) mark;

[0041] Nuclear dye: R 1 for COOEt or CH 3 or H; R 2 for CH 3 or CH 2 CH 3 or H; R 3 for CH 3 or CH 2 CH 3 or H.

[0042] Its synthetic method is as follows (with R 1 is COOEt; R 2 for CH 3 ; 3 for CH 2 CH 3 example):

[0043]

[0044] Overall yield: 17.5%. 1 H NMR (400MHz, deuterated DMSO): δ8.41(s,1H,ArH),7.91(s,1H,ArH),7.59(s,1H,ArH),7.23(s,2H,ArH),7.21( s,2H,ArH),4.38(m,2H,NHCH 2 ),4.30(m,2H,COOCH 2 CH 3 ),4.01(s,1H,NHCH 2 ),3.90(m,1H,C=NH),3.76(m,1H,HCl),3.35(m,2H,NCH 2 CH 3 ),3.01(s,3H,NCH 3 ),1.29(s,3H,COOCH 2 CH 3 ),1.12(s,3H,NCH 2 CH 3 ).

[0045] 3. Cell fixative:

[0046]4% PFA: 4% Paraformaldehyde...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for detecting residual cells in a single donor platelet product. The method comprises the following steps: (1) extracting a cell sample, and adding a staining antibody regent, so as to finish specific labeling of the cell; (2) horizontally spreading the cell sample, carrying out observation and counting through a fluorescence microscope, wherein the staining antibody regent contains Alexa Fluor(R)488 labeled CD41 and CD61 for realizing the staining of blood platelets, Alexa Fluor(R)594 labeled CD45 for realizing the staining of leukocytes and a cell nucleus dye for realizing the staining of leukocyte nucleuses. According to the scheme, by utilizing a measure for combining cell dye labeling with fluorescence imaging, the simultaneous detection of residual leukocytes, erythrocytes and blood platelets can be realized; and the method is applicable to the fluorescence microscopes and software calculation so as to realize automatic detection and counting; and the method is simple in operation and high in efficiency.

Description

technical field [0001] The invention relates to the detection field of platelet products, in particular to a method for detecting residual cells in apheresis platelet products. Background technique [0002] At present, platelet blood transfusion is one of the most important treatment methods for thrombocytopenia and various diseases, and has developed into the second most clinical component blood transfusion after red blood cell transfusion. However, ineffective platelet transfusions still occur frequently. Blood patients generally need multiple platelet transfusions. Ineffective transfusions are likely to occur after multiple transfusions of platelets. The main reason is the production of allotype antibodies. Studies have shown that, HLA antibody, HPA antibody and ABH antibody exist in most patients with ineffective platelet transfusion, and 80% of them have HLA antibody. The main reason for the antibody production is likely to be the stimulation of the patient's immune sy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
07 Dec 2018
Publication
CN108956431A
IPC
G01N15/14
CPC
G01N15/14; G01N2015/1006; G01N2015/1486
Inventors
胡佳