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A highly stable and rapid colorimetric detection reagent for ammonium

A high-stability, ammonium-based technology, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, can solve problems such as strong toxicity, poor stability, and complex synthesis of probe molecules. , to achieve the effect of convenient promotion and application and simple operation

Active Publication Date: 2021-04-20
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It overcomes the disadvantages of the existing colorimetric method for ammonium detection, such as strong toxicity, poor stability, and complex synthesis of probe molecules.

Method used

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  • A highly stable and rapid colorimetric detection reagent for ammonium
  • A highly stable and rapid colorimetric detection reagent for ammonium
  • A highly stable and rapid colorimetric detection reagent for ammonium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] At room temperature, weigh 100 mg of thymol blue and dissolve it in 1 L of ultrapure water to obtain a 0.1 g / L thymol blue aqueous solution, use a pipette to measure 5 mL of 0.1 g / L thymol blue aqueous solution, and 20 μL of ultrapure Dilute 10 times of 40% tetrabutylammonium hydroxide (TBAH) aqueous solution in water, stir with 5mL ultrapure water until uniformly mixed, and the stirring time is 10min, to obtain thymol blue and tetrabutylammonium hydroxide (TBAH) in aqueous solution Blue detection reagents with concentrations of 107 μM and 308 nM, respectively;

[0040] Take 3 mL of the prepared detection reagent and place it in a quartz cuvette, then add 30 μL of the prepared 1M ammonium root standard solution, and scan the UV-visible absorption spectrum with a UV-visible spectrophotometer. The spectra before and after the addition of ammonium root Figure such as figure 1 As shown, by comparing the spectrograms before and after the reaction, it can be seen that the ab...

Embodiment 2

[0042] At room temperature, weigh 100mg of thymol blue and dissolve it in 1L of ultrapure water to obtain a 0.1g / L thymol blue aqueous solution, take 9 glass bottles, and add 5mL of 0.1g / L thymol blue with a pipette gun water solution and 5mL ultrapure water, then measure 4μL, 6μL, 8μL, 10μL, 12μL, 14μL, 16μL, 18μL and 20μL of 40% TBAH aqueous solution in water that has been diluted 10 times, add it to 9 glass bottles, and stir until Mix evenly and stir for 20 minutes to obtain a thymol blue molar concentration of 107 μM and a tetrabutylammonium hydroxide (TBAH) molar concentration of 61.6nM, 92.4nM, 123.2nM, 154nM, 184.8nM, 215.6nM, 246.4nM, 277.2nM and 308nM blue detection reagent;

[0043] Measure 3 mL of the configured detection reagent and place it in a quartz cuvette, then add 30 μL of the prepared 1M ammonium standard aqueous solution, and use a UV-Vis spectrophotometer to scan the UV-Vis absorption spectrum. Different tetrabutylammonium hydroxide ( The reagent of TBAH...

Embodiment 3

[0045] At room temperature, weigh 100mg of thymol blue and dissolve it in 1L of ultrapure water to obtain a 0.1g / L thymol blue aqueous solution, take 17 10mL volumetric flasks, and add 40% dilute 10 times with ultrapure water with a pipette gun % tetrabutylammonium hydroxide (TBAH) aqueous solution in water 20μL, then measure 100μL, 200μL, 400μL, 600μL, 800μL, 1mL, 1.2mL, 1.4mL, 1.6mL, 1.8mL, 2mL, 2.5mL, 3mL, 3.5 Add 0.1g / L thymol blue aqueous solution of mL, 4mL, 4.5mL and 5mL into 17 volumetric flasks, dilute to volume with ultrapure water and shake well for 30min to obtain the molar concentration of tetrabutylammonium hydroxide (TBAH). 308nM and Thymol blue molar concentration is 2.14μM, 4.28μM, 8.56μM, 12.84μM, 17.12μM, 21.4μM, 25.68μM, 29.596μM, 38.52μM, 42.8μM, 53.5μM, 64.2μM, 74.9μM, 85.6 μM, 96.3μM and 107μM blue detection reagents;

[0046] Take 3 mL of the prepared detection reagent and place it in a quartz cuvette, then add 30 μL of the prepared 1M ammonium standar...

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Abstract

The invention provides a reagent for highly stable and rapid colorimetric detection of ammonium radicals. The reagent is prepared from thymol blue, tetrabutylammonium hydroxide and ultrapure water as a solvent. It is mainly used under alkaline conditions. Roots can be detected by rapidly turning a blue thymol blue solution to yellow. The reagent can change color with the naked eye within 1 s after being in contact with ammonium radicals. It can detect ammonium radical ions in the environment and ammonium salt residues in non-standard explosive raw materials, and shows an extremely low detection limit for ammonium radicals (naked eye detection limit of 10 μM and UV-Vis detection limit of 51 nM) and very high selectivity. It has the characteristics of no complicated organic synthesis, time-saving and labor-saving, green and non-toxic, easy to operate, fast color development, high sensitivity, and low cost.

Description

technical field [0001] The invention belongs to the field of detection of environmental and non-standard explosive raw materials, and provides a green and non-toxic colorimetric reagent for detecting ammonium radicals which can not only be stored stably. The reagent has low detection limit, strong anti-interference and short reaction time, and can fully realize the purpose of low-cost, real-time colorimetric detection of ammonium radicals. Background technique [0002] With the rapid development of industry and agriculture, a large amount of nitrogen-containing compounds (such as ammonium radicals) have been discharged into surface and groundwater (Sensors and Actuators B: Chemical, 2018, 256: 167-175). Among these nitrogen-containing compounds, dissolved ammonium is a very important component of the nitrogen cycle process in the ecological environment. Excessive ammonium root will lead to the rapid proliferation of algae in water, and the excessive proliferation of algae w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 窦新存王广发蔡珍珍
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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