A kind of culture method of masson pine tissue culture subculture bud proliferation and rejuvenation
A technology of subculture and culture method, which is applied in the field of tissue culture seedling cultivation, masson pine tissue culture subculture bud proliferation and rejuvenation, and can solve the problems of low effective bud proliferation coefficient, poor physiological activity of subculture buds, and difficulties in subculture proliferation and other problems, to achieve the effects of high subculture multiplication coefficient, shortening the subculture period, and slowing down the excessive lignification of sprouts
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Embodiment 1
[0025] In accordance with the rapid growth of the Massamatsu group, routine disinfection, vaccination and subsequent culture are carried out, and the sect of 3 / sub-cycle is 40 days, the coniferous stretches, the leaf sey is long, 3.5-4.5 cm is higher. As the experimental material, the bud was carried out by the experimental material.
[0026] Select 500 mL of the colorless glass triangle bottle to add 100 ml of solid medium I, after the routine high temperature and high pressure, a single cut is growing and strong, high 2.5-3.5 cm In the contingenulation of the solid medium I, the temperature is 25 ± 1 ° C, the light source is 650 nm red light: 450 nm Blu-ray = 3: 5 red-blue LED lamp, light intensity 3000-3500 lx, 7 days were cultured under the conditions of the illumination time 16 h / d, and the buds were formed. Wherein, the solid medium I has a raw material content of: META-TOPOLIN (MT) 0.5 mg · L -1 6-ba 1.0 mg · L -1 , Sucrose 25000 mg · L -1, Glucose 1000 mg · L -1 , Joan...
Embodiment 2
[0030] In accordance with the rapid growth of the Massonus Song group, routine disinfection, vaccination and subsequent cultures were carried out, and 40 days of 5 / subsequent cycle, the coniferous stretches, the length of the leaves, 3.5-4.5 cm high As the experimental material, the bud was carried out by the experimental material.
[0031] Select 500 mL of the colorless glass triangle bottle to add 100 ml of solid medium I, after the routine high temperature and high pressure, a single cut is growing and strong, high 2.5-3.5 cm In the contingenulation of the solid medium I, the temperature is 25 ± 1 ° C, the light source is 650 nm red light: 450 nm Blu-ray = 3: 5 red-blue LED lamp, light intensity 3000-3500 lx, 8 days were cultured under the conditions of the illumination time 16 h / d, and the buds formed were formed. Wherein, the solid medium I has a raw material content of: META-TOPOLIN (MT) 0.5 mg · L -1 6-ba 1.5 mg · L -1 , Sucrose 25000 mg · L -1 , Glucose 1000 mg · L -1 ...
Embodiment 3
[0035] According to the high-ranging local standards of the Massonus Song Group, routine disinfection, vaccination and subsequent cultivation are carried out, and 40 days of 10th / subsequent cycle, the conifer, the leaves are long, 3.5-4.5 cm high As the experimental material, the bud was carried out by the experimental material.
[0036] Select 500 mL of the colorless glass triangle bottle to add 100 ml of solid medium I, after the routine high temperature and high pressure, a single cut is growing and strong, high 2.5-3.5 cm In the contingenulation of the solid medium I, the temperature is 25 ± 1 ° C, the light source is 650 nm red light: 450 nm Blu-ray = 3: 5 red-blue LED lamp, light intensity 3000-3500 lx, 10 d was cultured under the condition of 16 h / d, and the buds were formed. Wherein, the solid medium I is: meta-topolin (MT) 0.8 mg · L -1 6-ba 1.0 mg · L -1 , Sucrose 25000mg · L -1 , Glucose 1000 mg · L -1 , Joan 7000 mg · L -1 And improve MS medium.
[0037] In the ste...
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