A kind of culture method of masson pine tissue culture subculture bud proliferation and rejuvenation

A technology of subculture and culture method, which is applied in the field of tissue culture seedling cultivation, masson pine tissue culture subculture bud proliferation and rejuvenation, and can solve the problems of low effective bud proliferation coefficient, poor physiological activity of subculture buds, and difficulties in subculture proliferation and other problems, to achieve the effects of high subculture multiplication coefficient, shortening the subculture period, and slowing down the excessive lignification of sprouts

Active Publication Date: 2021-07-02
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the easy physiological aging of Pine massoniana in the in vitro tissue culture process, the physiological activity of the subcultured buds is poor, the subculture proliferation is difficult, the effective bud proliferation coefficient is low, and vitrification and browning are obvious, which seriously limits the excellent quality of Pine massoniana. Development of Industrialization of Germplasm Asexual Seedling Breeding

Method used

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  • A kind of culture method of masson pine tissue culture subculture bud proliferation and rejuvenation
  • A kind of culture method of masson pine tissue culture subculture bud proliferation and rejuvenation
  • A kind of culture method of masson pine tissue culture subculture bud proliferation and rejuvenation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] In accordance with the rapid growth of the Massamatsu group, routine disinfection, vaccination and subsequent culture are carried out, and the sect of 3 / sub-cycle is 40 days, the coniferous stretches, the leaf sey is long, 3.5-4.5 cm is higher. As the experimental material, the bud was carried out by the experimental material.

[0026] Select 500 mL of the colorless glass triangle bottle to add 100 ml of solid medium I, after the routine high temperature and high pressure, a single cut is growing and strong, high 2.5-3.5 cm In the contingenulation of the solid medium I, the temperature is 25 ± 1 ° C, the light source is 650 nm red light: 450 nm Blu-ray = 3: 5 red-blue LED lamp, light intensity 3000-3500 lx, 7 days were cultured under the conditions of the illumination time 16 h / d, and the buds were formed. Wherein, the solid medium I has a raw material content of: META-TOPOLIN (MT) 0.5 mg · L -1 6-ba 1.0 mg · L -1 , Sucrose 25000 mg · L -1, Glucose 1000 mg · L -1 , Joan...

Embodiment 2

[0030] In accordance with the rapid growth of the Massonus Song group, routine disinfection, vaccination and subsequent cultures were carried out, and 40 days of 5 / subsequent cycle, the coniferous stretches, the length of the leaves, 3.5-4.5 cm high As the experimental material, the bud was carried out by the experimental material.

[0031] Select 500 mL of the colorless glass triangle bottle to add 100 ml of solid medium I, after the routine high temperature and high pressure, a single cut is growing and strong, high 2.5-3.5 cm In the contingenulation of the solid medium I, the temperature is 25 ± 1 ° C, the light source is 650 nm red light: 450 nm Blu-ray = 3: 5 red-blue LED lamp, light intensity 3000-3500 lx, 8 days were cultured under the conditions of the illumination time 16 h / d, and the buds formed were formed. Wherein, the solid medium I has a raw material content of: META-TOPOLIN (MT) 0.5 mg · L -1 6-ba 1.5 mg · L -1 , Sucrose 25000 mg · L -1 , Glucose 1000 mg · L -1 ...

Embodiment 3

[0035] According to the high-ranging local standards of the Massonus Song Group, routine disinfection, vaccination and subsequent cultivation are carried out, and 40 days of 10th / subsequent cycle, the conifer, the leaves are long, 3.5-4.5 cm high As the experimental material, the bud was carried out by the experimental material.

[0036] Select 500 mL of the colorless glass triangle bottle to add 100 ml of solid medium I, after the routine high temperature and high pressure, a single cut is growing and strong, high 2.5-3.5 cm In the contingenulation of the solid medium I, the temperature is 25 ± 1 ° C, the light source is 650 nm red light: 450 nm Blu-ray = 3: 5 red-blue LED lamp, light intensity 3000-3500 lx, 10 d was cultured under the condition of 16 h / d, and the buds were formed. Wherein, the solid medium I is: meta-topolin (MT) 0.8 mg · L -1 6-ba 1.0 mg · L -1 , Sucrose 25000mg · L -1 , Glucose 1000 mg · L -1 , Joan 7000 mg · L -1 And improve MS medium.

[0037] In the ste...

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Abstract

The invention provides a culture method for the proliferation and rejuvenation of subcultured shoots of Pine massoniana tissue culture, including the procedures of selection of subcultured buds, induction of axillary buds and rejuvenation culture, and selecting the subcultured single subcultured Pine massoniana after conventional disinfection, inoculation and subculture. The buds were transferred to the subculture bottle containing solid medium Ⅰ for axillary bud induction, and then the double-layer biphasic culture method was adopted, and liquid medium Ⅱ was added to the original subculture bottle for rejuvenation culture of the subculture buds, and vigorous growth vigor was obtained. Masson pine succession cluster buds. The invention not only simplifies the transfer steps in the traditional subculture of Pinus massoniana tissue culture, but also obtains the secondary shoots of Pinus massoniana with high subculture multiplication coefficient, strong sprout vitality, and not easy to age, and realizes the large-scale production of excellent Pinus massoniana germplasm The rapid propagation and utilization of in vitro tissue culture has good economic, social and ecological benefits.

Description

Technical field [0001] In the field of plant reproductive technology, the invention relates to the cultivation of seedling techniques, especially a cultivation and completion of ponymuno culture. Background technique [0002] Horsescong ( Pinus Massonianan my country, my country is one of the important tree of rolling, paper-bearing role forests and feminin forests in my country. It is extremely broad in China's distribution. As a tree species having a high value of comprehensive utilization, the promotion potential is high, but also can be used to produce three plates, papermaking and chemical fiber industrial manufacturing, but also to produce industrial raw materials such as rosin, train oil. In recent years, with the increasingly growing life demand and the shortage of wood resource shortages and oil-like non-renewable resources, the sustainable development of the ecology, economy and society is increasingly being concerned. Based on the issue of ecological security, resource...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 姚瑞玲王胤
Owner GUANGXI FORESTRY RES INST
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