Bacillus and its use

A technology of Bacillus tequila and Bacillus tequila, applied in the field of microorganisms, can solve the problems of difficult survival, colonization, poor soil-borne disease control effect, etc., and achieve the effects of easy availability, good control effect and low price

Active Publication Date: 2018-12-14
山东九甲农业有限公司
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Problems solved by technology

[0004] Soil salinization in my country is serious, and salinized soil accounts for about 1/3 of the cultivated land. In view of the characteristics of large population and little land, these salinized soils are still used by people to grow salt-tolerant crops. At present, the development of microorganism

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  • Bacillus and its use
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  • Bacillus and its use

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Example Embodiment

[0024] The following specific examples are further descriptions of the methods and technical solutions provided by the present invention, but should not be construed as limiting the present invention.

[0025] 1. Classification and identification of Bacillus strains

[0026] Bacillus HTTA-X0189 strain, in solid peptone medium: peptone 4g, yeast powder 2g, NaCl, 15-25g, KCl 0.2-0.4g, MgCl 2 ·7H 2 O 1.5-2.5g, water 1000ml, pH 7.0, agar 16-19g, 25℃-27℃, cultivate for 2-3 days, observe and record the changes of colony morphology, color, etc., observe the morphology of bacteria under microscope, and test the physiology of bacteria by conventional methods Biochemical reaction. The strains were cultured by the above methods, using the universal primers of the bacterial 16SrDNA gene, the PCR amplified products were sequenced by conventional methods, and compared through the BLAST program of NCBI, sequence homology was analyzed and the genetic relationship branch tree (see figure 1 ) Relat...

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Abstract

The invention discloses bacillus and its use. The bacillus is Bacillus tequilensis and has a strain number of HTTA-X0189 and a preservation number of CGMCC No. 15770. The bacillus can be used for preventing and treating plant diseases and inhibiting bacteria and in pesticide. The Bacillus tequilensis has a low artificial culture fermentation cost, utilizes a culture material with a low price, hasgood prevention and treatment effects on plant diseases and has normal cultivated land and saline soil prevention and treatment effects exceeding or reaching to the level of a chemical medicament.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacillus and its application in pesticides. Background technique [0002] With the increasing popularization of protected cultivation of vegetables and fruits, it has also caused serious occurrence of diseases regardless of seasons. Facility cultivation can provide off-season agricultural products and increase yield, but the normal period of warmth and humidity and continuous cropping cultivation will make Botrytis cinerea The base of pathogens is increasing year by year. Botrytis cinerea not only mainly harms tomatoes, but also harms more than 20 kinds of crops such as eggplant, pepper, cucumber, gourd, etc., causing stem and leaf withering, rotten flowers, and rotten fruits, directly affecting yield, reducing yield by 20-20%. 50%, becoming a common and relatively difficult disease of crops in protected areas. A large number of chemical pesticides pollute v...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 陈杰袁静黄晓慧
Owner 山东九甲农业有限公司
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