Bacillus and its use
A technology of Bacillus tequila and Bacillus tequila, applied in the field of microorganisms, can solve the problems of difficult survival, colonization, poor soil-borne disease control effect, etc., and achieve the effects of easy availability, good control effect and low price
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[0024] The following specific examples are further descriptions of the methods and technical solutions provided by the present invention, but should not be interpreted as limiting the present invention.
[0025] 1. Classification and identification of Bacillus strains
[0026] Bacillus HTTA-X0189 strain, in solid peptone medium: peptone 4g, yeast powder 2g, NaCl, 15-25g, KCl 0.2-0.4g, MgCl 2 ·7H 2 O 1.5-2.5g, water 1000ml, pH 7.0, agar 16-19g, culture at 25°C-27°C for 2-3 days, observe and record changes in colony shape, color, etc., observe the shape of bacteria with a microscope, and test their physiology by conventional methods for bacterial identification biochemical reaction. Bacterial strains were cultivated by the above-mentioned method, using the general primers of bacterial 16SrDNA gene, sequencing of PCR amplification products by conventional method, and comparing by NCBI BLAST program, analyzing sequence homology and making phylogenetic branch tree (see figure 1 ...
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