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Plant seed pregermination method under cadmium stress and used pregermination agent

A plant seed and germination agent technology, applied in the direction of seed immunity, seed and rhizome treatment, application, etc., can solve the problems of inability to improve seed quality, synergistic stress, etc.

Inactive Publication Date: 2018-12-18
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the addition of chemical substances, it may provide a small amount of energy to the seeds and improve the germination potential of the seeds, but it cannot improve the quality of the seeds
At the same time, given the biocompatibility of chemical substances, high concentrations may form a synergistic stress with cadmium

Method used

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  • Plant seed pregermination method under cadmium stress and used pregermination agent
  • Plant seed pregermination method under cadmium stress and used pregermination agent
  • Plant seed pregermination method under cadmium stress and used pregermination agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The separation and purification of embodiment 1 bacterial siderophilic element

[0033] Burkholderia pyrrole JK-SH007 strain (hereinafter referred to as JK-SH007 strain) and its cultivation method are the same as CN101555458A.

[0034] The JK-SH007 strain was cultured in 100mL MSA medium for 48h, then centrifuged at 5000×g at 37°C, and the supernatant was taken. The supernatant was acidified to pH 2 with 2M hydrochloric acid, passed through a macroporous resin XAD-1600N column, methanol was used as the mobile phase, and the fractions were collected in sections, and the absorbance at 280nm was measured, and the fractions with effective absorption values ​​were combined, concentrated and evaporated at 37°C Dry. The crystals purified by the macroporous resin were dissolved in a small amount of methanol, and passed through a Sephadex LH-20 column, with methanol as the mobile phase, to collect fractions, measure the absorbance at 280nm, and combine the fractions with effect...

Embodiment 2

[0035] Embodiment 2 germination test

[0036] In order to ensure the interference of metal ions, especially iron ions, on the test results in the test, the glass instruments used in the test were soaked in 1mol / L hydrochloric acid overnight and rinsed repeatedly with deionized water.

[0037] Seeds of cucumber (Cucumis sativus L.cv. Jinyan No. 4) and tomato (Lycopersicon esculentumMill.cv. Huangsheng) were sterilized with 20% NaClO for 10 minutes, and then washed with plenty of tap water. Seed in series concentrations of CdSO 4 The solution (Table 1) was soaked for 1 h, transferred to a petri dish with double-layer filter paper and added with 5 mL of a siderophilic aqueous solution of serial concentrations (Table 1), 30 grains / dish, and induced germination at 25°C in the dark. After 3 days (cucumber), 5 days (tomato), germinated seeds were counted to calculate germination rate and vigor index.

[0038] Table 1 Effects of different metal concentrations and different siderophi...

Embodiment 3

[0041] Example 3 Different sterilization methods, different concentrations of siderophilic iron + 200 μmol / L CdSO 4 germination test

[0042] Cucumber and tomato seeds were surface sterilized with 20% NaClO or 75% alcohol for 10 minutes, and then rinsed with plenty of tap water. Dilute the seeds in 200 μmol / L CdSO 4The solution was soaked for 1 hour, transferred to a petri dish with double-layer filter paper and added with 5 mL of a siderophilic aqueous solution (Table 2) at a series concentration, 30 grains / dish, and induced germination in the dark at 25°C. After 3 days (cucumber), 5 days (tomato), germinated seeds were counted to calculate germination rate and vigor index.

[0043] Table 2 Effects of different sterilization methods and different siderophilic treatments on germination:

[0044]

[0045] The result is as image 3 , Figure 4 and shown in Table 2; where, image 3 is the germination status of cucumber seeds under 20% NaClO sterilization treatment under ...

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Abstract

The invention discloses a plant seed pregermination method under cadmium stress and a used pregermination agent. The plant seed pregermination method is characterized in that siderophore is used in the plant seed pregermination process. The high-yield siderophore strain fermentation liquid is separated and purified; to obtain the bacterium siderophore purified product is obtained. The siderophoreis used for preparing a dilute solution as a plant seed pregermination agent of tomatoes, cucumbers and the like under the cadmium stress; the germination rate or the young seedling vitalityactivityindex of plant seeds of the tomatoes, cucumbers and the like under the cadmium stress can be effectively improved; when the cadmium concentration is 200 mumol / L, the tomato germination rate is improved by about 23.1 percent; the cucumber vitalityactivity index is improved by about 27 percent; meanwhile, the pregermination effect of the siderophore dilute solution on the tomato seeds is not interfered by other metal ions.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for accelerating germination of plant seeds under cadmium stress and a germination agent used therefor. Background technique [0002] Heavy metal elements generally refer to metal elements whose elemental density is greater than 4500kg / m3 under standard conditions. Heavy metal pollution refers to environmental pollution caused by heavy metals or their compounds, mainly caused by human factors such as mining, waste gas discharge, sewage irrigation, and the use of products with heavy metals exceeding the standard. According to the national survey of sewage irrigation areas organized by the Ministry of Agriculture, the current area of ​​sewage irrigation areas in my country has reached 1.4 million hm 2 , with the largest area polluted by cadmium. According to incomplete statistics, the area of ​​farmland polluted by Cd in my country has reached 2.8×10hm 2 . The ...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01C1/08
CPCA01C1/00A01C1/08
Inventor 叶建仁闵莉静吴小芹
Owner NANJING FORESTRY UNIV
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