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Cross-flow filtration system for enriching circulating tumor cells

A technology for cross-flow filtration and tumor cells, applied in the field of cross-flow filtration systems, can solve problems such as high cost, insufficient purity of enriched cells, and difficult design, and achieve the effects of simple operation, favorable promotion, and great social benefits

Active Publication Date: 2018-12-18
SHANGHAI POLARIS BIOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The microfluidic chip method can increase the amount of sample processing while also improving the ability to enrich rare tumor cells, but this method is not only difficult to design, but also high in cost, and the purity of the enriched cells is not high enough

Method used

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  • Cross-flow filtration system for enriching circulating tumor cells
  • Cross-flow filtration system for enriching circulating tumor cells
  • Cross-flow filtration system for enriching circulating tumor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] figure 1As shown, the cross-flow filtration system for enrichment of circulating tumor cells of the present invention includes a volumetric flask 1 of 500 ml of cell fluid to be filtered, and three peristaltic pumps: a first peristaltic pump 2-1, a second peristaltic pump 2-2, and a third peristaltic pump Pump 2-3, 50 milliliter centrifuge tube 3, filter chip 7, 500 milliliter volumetric flask 5 of cell fluid filtered by circulating cross-flow,

[0045] The first peristaltic pump 2-1 is arranged on the pipeline (13) between the 500 milliliter volumetric flask 1 of the cell solution to be filtered and the 50 milliliter centrifuge tube 3; the second peristaltic pump 2-2 is arranged on the 50 milliliter centrifuge tube 3 and the chip liquid inlet end 4 of the filter chip 7 on the pipeline (14); the third peristaltic pump 2-3 is arranged on the waste liquid end 8 of the filter chip 7 and the cell fluid 500 filtered by circulating cross-flow On the pipeline (15) between the...

Embodiment 2

[0063] Pancreatic cancer cell line Panc-1 purchased from the stem cell bank of the Institute of Biochemical Cells, Chinese Academy of Sciences

[0064] Operate with embodiment 1.

[0065] Embodiment 2 is compared with microfluidic chip filtration, comprises the following steps:

[0066] The pancreatic cancer cell line Panc-1 was digested with trypsin from the T25 cell culture flask, the pancreatic cancer cell line changed from the adherent state to the suspension state, and then centrifuged at 1500 rpm for 10 minutes, the cells were precipitated at the bottom, and the supernatant was removed . Cyclic cross-flow filtration is carried out by the method of the invention described above. Compared with the method of centrifugation enrichment.

[0067]

[0068] It can be seen from the table that the cross-flow filtration invention is much more efficient in sample recovery than centrifugal enrichment, and at the same time the sample processing time is greatly reduced, saving ti...

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Abstract

The invention provides a cross-flow filtration system for enriching circulating tumor cells. The system comprises: a tumor cell sap-to-be-filtered volumetric flask (1); three peristaltic pumps which are a first peristaltic pump (2-1), a second peristaltic pump (2-2) and a third peristaltic pump (2-3) respectively; a centrifugal tube (3); a filtering chip (7); and a cross-flow filtered tumor cell sap volumetric flask (5). A process represented by figure 1 is a new method provided on the basis of filter membrane filtration and a micro-fluidic fluid principle. The cross-flow filtration is characterized in that the flow direction of a water phase is parallel to the surface of the filter membrane to form a large pressure on the surface of the filter membrane, so cells enter filter holes, and are filtered and screened, and the activity of the cells is not affected after the filtration in order to achieve the efficient enrichment effect. The cross-flow filtration system for enriching circulating tumor cells reaches the efficient enrichment effect. The system has the advantages of simplicity in sample treatment, high recovery rate of the sample, simplicity in operation, convenience in use,facilitation of promotion, good clinic application prospect and great social benefit.

Description

technical field [0001] The invention relates to medical equipment, in particular to a cross-flow filtration system for enrichment of circulating tumor cells. Background technique [0002] Circulating tumor cells are a type of cells that are shed from solid tumor lesions and enter the blood and lymphatic system, and are the main cause of tumor recurrence and metastasis in patients. Clinical monitoring of circulating tumor cells can predict the risk of tumor recurrence earlier than conventional imaging methods, and compared with tumor tissue sampling, the sampling method of circulating tumor cells is more convenient, faster, less invasive, and can be repeatedly sampled. However, due to the extremely small number of circulating tumor cells in the blood, every 10 8 -10 9 There is only one circulating tumor cell per peripheral blood cell. At present, more mature enrichment methods can be divided into two categories. One is enrichment based on molecular markers on the surface of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D63/08G01N1/34G01N1/40
CPCB01D63/087B01D2311/04B01D2315/10G01N1/34G01N1/4005G01N1/4077G01N2001/4016B01D2311/2676
Inventor 王宇翀邹立沈美萍
Owner SHANGHAI POLARIS BIOLOGY CO LTD
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