A polypeptide fragment specifically binding to the serum of Babesia patients and its application

A technique for polypeptide fragments and babesiosis, which is applied in the field of biomedicine and clinical applications, can solve the problems of complex preparation process, unstable activity, and high cost, improve specificity and sensitivity, and overcome missed detection and misdiagnosis of parasites Effect

Active Publication Date: 2022-01-18
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, BMSA is prepared by worm purification and Escherichia coli recombinant expression. The preparation process is complicated, the cost is high, and the activity is unstable. In addition, part of the amino acid sequence in the full-length BMSA protein has been confirmed to contain no antigenic epitopes recognized by patient serum. Therefore, using the full-length BMSA protein as a serum diagnostic antigen is prone to cross-reaction

Method used

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  • A polypeptide fragment specifically binding to the serum of Babesia patients and its application
  • A polypeptide fragment specifically binding to the serum of Babesia patients and its application
  • A polypeptide fragment specifically binding to the serum of Babesia patients and its application

Examples

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Effect test

Embodiment 1

[0021] Example 1 The biological characteristics of Babesia microti merozoite surface antigen BMSA

[0022] According to the Babesia microti merozoite surface antigen BMSA gene sequence (such as figure 1 Shown) The deduced protein is composed of 326 amino acids (as shown in Sequence 2), and the size is 35.117kDa; the isoelectric point pI is predicted to be 5.21 by ExPAS software, and the 302nd amino acid is predicted by the PredGPI software to be the GPI anchor site, SignalP4 .1 The software predicts that the N-terminal 24 amino acids are signal peptides (such as figure 2 shown); TMPred software predicts the transmembrane segment from extracellular to intracellular, from amino acid 310 to 326 (Score2066) (eg image 3 shown), the PotParam software predicts that negatively charged amino acids account for 53%, and the proportion of positively charged amino acids is 43%; homology modeling predicts the three-dimensional structure of BMSA as Figure 4 shown.

Embodiment 2

[0023] Example 2 Epitope Analysis and Verification of the Babesia vole merozoite surface antigen BMSA

[0024] According to the Disco Tope 2.0 software, there are 6 antigenic epitopes (as shown in Table 1) for the B-cell epitope of the Babesia vole merozoite surface antigen BMSA, and the peptide synthesis of these 6 epitopes is carried out by artificial peptide synthesis. Synthesize; then screen and identify the epitope by indirect ELISA method through the serum of mice infected with Babesia, the method is as follows: Add 100 μL / well Coating Buffer (0.5 μg of artificially synthesized BMSA protein polypeptide) to the 96-well microtiter plate , in a wet box, coated overnight at 4°C; remove the coating solution, wash the plate with PBST, add 1% skimmed milk-PBS at room temperature, and block for 1 hour; add double-diluted Babesia-infected mouse serum and BMSA After immunization, mouse serum was 100 μL / well (1:3200 1:6400, PBS doubling dilution), incubated at room temperature for ...

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Abstract

The invention belongs to the technical field of biomedicine and clinical application, and relates to a polypeptide fragment specifically binding to the serum of Babesia patients and its amino acid sequence. A series of polypeptide fragments are obtained through bit analysis, and the polypeptide fragments are prepared by polypeptide synthesis, which can be used to prepare serological immunodiagnostic reagents for babesiosis. It is suitable for improving the specificity and sensitivity of babesiosis diagnosis.

Description

technical field [0001] The invention relates to the technical field of biomedicine and clinical application, in particular to a polypeptide fragment specifically binding to the serum of Babesia patients, its amino acid sequence and its application. Background technique [0002] The prior art discloses that Babesiosis (Babesiosis) is a new zoonotic disease caused by the infection of the protozoa that parasitizes in red blood cells——Babesia. Studies have shown that the disease is mainly transmitted through blood sucking after the arthropod tick bites the host, and can also be transmitted through blood transfusion and mother-to-child transmission. Clinical practice shows that after Babesia infection, according to the host's own immune status and species, it may manifest as asymptomatic, malaria-like symptoms and dangerous symptoms; the general symptoms of Babesia infection in immune-competent persons are fatigue, fever, Headache, chills, night sweats, anemia, loss of appetite,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/44G01N33/68G01N33/569
CPCG01N33/56905G01N33/6854C07K14/44G01N2469/20
Inventor 程训佳满素勤付永锋
Owner FUDAN UNIV
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