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Biomarker long-chain non-coding RNA LINC02447 and kit for predicting prognosis of colon cancer

A biomarker, long-chain non-coding technology, which is applied in the field of biomarker long-chain non-coding RNA LINC02447 and kits, can solve problems that have not been reported, and achieve improved survival rate, postoperative quality of life, and far-reaching clinical significance Effect

Inactive Publication Date: 2018-12-18
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whether LINC02447 can be used as a new tumor marker to predict the prognosis of patients with colon cancer has not been reported

Method used

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  • Biomarker long-chain non-coding RNA LINC02447 and kit for predicting prognosis of colon cancer
  • Biomarker long-chain non-coding RNA LINC02447 and kit for predicting prognosis of colon cancer
  • Biomarker long-chain non-coding RNA LINC02447 and kit for predicting prognosis of colon cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The biomarker used to predict the prognosis of colon cancer is long-chain non-coding RNA LINC02447, and the nucleic acid sequence of the long-chain non-coding RNA LINC02447 is shown in SEQ NO:1.

[0019] The preparation for predicting the prognosis of colon cancer is a real-time fluorescent quantitative PCR detection kit, which includes specific primers for detecting the expression of the target gene LINC02447, and the primer sequences are shown in SEQ NO:2 and SEQNO:3.

[0020] The real-time fluorescent quantitative PCR detection kit for predicting the prognosis of colon cancer also includes specific primers for the expression of the internal reference gene TUBA1A, and the primer sequences are shown in SEQ NO:4 and SEQ NO:5.

[0021] Preparation of reagents for detecting the expression of LINC02447 is used to prepare a kit for the prognosis of patients with colon cancer (for 50 reactions), and the required reagents include: SYBR Green qPCR Mix 500 μL, 3 μM target gene L...

Embodiment 2

[0025] Detection of LINC02447 in Tissue Samples

[0026] 1. Collect the colon cancer tumor tissue or normal control tissue to be tested, wash it with normal saline, put it into a cryopreservation tube filled with a solvent that inhibits RNA degradation, and put it in a -80°C refrigerator for later use.

[0027] 2. Extraction of RNA from tissues

[0028] (1) Add liquid nitrogen to the mortar first, then cut the tissue into small pieces and grind it into powder in liquid nitrogen, take 100 mg of tissue powder with a liquid nitrogen pre-cooled spoon and add it to the EP tube filled with 1 ml of Trizol solution middle. The total volume of tissue powder should not exceed 10% of the volume of Trizol used, and it should be thoroughly mixed evenly.

[0029] (2) Leave at room temperature for 5 minutes, then add 200 μL of chloroform, tightly cap the EP tube and shake vigorously for 0.5 minutes. Centrifuge at 12,000 rpm for 10 minutes.

[0030] (3) Take the upper aqueous phase into a...

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Abstract

The invention aims to provide a biomarker long-chain non-coding RNA LINC02447 and kit for predicting the prognosis of colon cancer to predict the prognostic lifetime of colon cancer patients. The preparation for predicting the prognosis of colon cancer is a real-time fluorescent quantitative PCR detection kit. The kit contains specific primers for detecting the expression of the target gene LINC02447 and specific primers for detecting the expression of the internal reference gene TUBA1A, and also includes real-time fluorescent quantitative SYBR dye and RNA enzyme-free water. By means of fluorescent quantitative PCR and survival curve analysis, the invention finds that the LINC02447 derived from colon cancer tissue is related to the survival rate of patients, and the higher the content is,the higher the survival rate is. The method provides powerful technical support for analysis predicting the prognostic lifetime of colon cancer patients, is conducive to enhancing the postoperative quality of life of colon cancer patients, formulating the postoperative therapeutic scheme and improving the survival rate, thus having far-reaching clinical significance.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a biomarker long-chain non-coding RNA LINC02447 and a kit for predicting the prognosis of colon cancer. Background technique [0002] Colon cancer is one of the most common malignant tumors of the gastrointestinal tract, and its incidence has been rising continuously, which is related to factors such as changes in dietary structure, improvement of living standards, and population aging. Colon cancer ranks third among male malignancies and second among female malignancies. Western Europe and North America were 43 / 100,000 and 45 / 100,000 respectively. The incidence of colon cancer in my country is 13 / 100,000. According to the existing relevant clinical investigation results, the current 5-year survival rate of colon cancer patients after surgery is about 50%, and the mortality rate is about 80%. At present, the main treatment for colon cancer is surgery, and chemo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/686C12N15/113
CPCC12Q1/6886C12Q1/686C12Q2600/118C12Q2600/158C12Q2600/178C12Q2561/113C12Q2563/107C12Q2545/114
Inventor 李兆明
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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