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A method for preparing ginkgolic acid in combination

A technology of ginkgolic acid and total ginkgolic acid, which is applied in the separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve problems such as lack of reference significance, complex composition of compounds, and unclear content, and achieve cost-effective, easy-to-operate, high efficiency effect

Active Publication Date: 2021-03-19
SHANDONG ANALYSIS & TEST CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extraction and separation of traditional Chinese medicine, the compounds contained in it are complex and the content is not clear
Therefore, the exploration of the solvent system is a complex and arduous task, and the extraction methods in the prior art often have no reference significance

Method used

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  • A method for preparing ginkgolic acid in combination
  • A method for preparing ginkgolic acid in combination
  • A method for preparing ginkgolic acid in combination

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0039] 1. Sample Extraction

[0040]Separate the testa of ginkgo fruit from the ginkgo fruit, place the testa in a cool place to dry, and extract 5 kg of the dried testa with 10 L of petroleum ether (boiling range 60-90 ° C) for 3 times, each time for 2 hours, and then The extract was filtered using a vacuum filter unit. All the extracts were combined and evaporated to dryness at 55° C. to obtain 247 g of total ginkgolic acid extract, which was stored in a refrigerator at 4° C. for further separation and purification by zonal countercurrent chromatography.

[0041] 2. β-cyclodextrin-ginkgolic acid inclusion complex

[0042] Take 2L of distilled water, add 30g of β-cyclodextrin, and stir magnetically at 60°C at a speed of 1000 rpm until it is completely dissolved, then add 10g of total ginkgolic acid extract, stir at 60°C for 48 hours in the dark, and evaporate the solution at 40°C. Dry the water to get β-cyclodextrin-ginkgolic acid inclusion compound.

[0043] 3. Separation...

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Abstract

The invention relates to a method for separating ginkgolic acid monomer from total ginkgolic acid extract by a pH (potential of hydrogen)-zone countercurrent chromatography method. The method has theadvantages that the hydrophilicity is improved by the type of using beta-cyclodextrin to coat the ginkgolic acid, so as to improve the distribution ratio of extracts in two phases; a two-phase solventsystem is n-heptane / ethyl acetate / methanol / water (2:1:1.5:1, v / v); the five types of high-purity ginkgolic acid monomer compounds, such as ginkgoneolic acid, ginkgolic acid, heptadecane diene ginkgolic acid, hydrogenated ginkgolic acid, and heptadecane monomer ginkgolic acid, can be separated out at one time; the preparation method is simple, the efficiency is high, the cost is economic, the preparation method is suitable for industrialized production, and the important economic meaning is realized.

Description

technical field [0001] The invention relates to a method for separating and purifying active ingredients of traditional Chinese medicine, specifically, using pH-zone countercurrent chromatography to separate ginkgo acid monomer compounds (ginkgolic acid, ginkgoic acid, heptadecanadiene ginkgolic acid, hydrogenated ginkgoic acid, decanoic acid, etc.) Hepta-ene ginkgolic acid) method. Background technique [0002] Ginkgolic acid compounds are another important medicinal ingredient besides flavonoids and lactones in the middle leaves, ginkgo and pericarp of Ginkgo biloba, which are derivatives of 6-alkenyl salicylic acid. Modern pharmacological studies have shown that ginkgolic acids have antiviral, antibacterial, anti-inflammatory, anti-cancer, anti-parasitic and other pharmacological activities. Since the ginkgolic acid component is a low-polarity structural analogue, it is difficult to separate. The traditional separation methods of monomer components include silica gel co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C65/19C07C65/05C07C51/42C07C51/47C07C51/48
CPCC07C51/42C07C51/47C07C51/48C07C65/19C07C65/05
Inventor 崔莉王晓闫慧娇耿岩玲刘伟付瑞明
Owner SHANDONG ANALYSIS & TEST CENT
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