Kit for testing small and dense low-density lipoprotein cholesterin

Abstract

The invention discloses a kit for testing small and dense low-density lipoprotein cholesterin. The kit comprises two independent liquid reagents R1 and R2, wherein the reagent R1 comprises the following components of 100mmol / L of Good's buffer solution, 1600U / L of CHE (cholesterol esterase), 600U / L of CO (cholesterol oxidase), 2700U / L of phospholipase, 1200KU / L of catalase, and 2mmol / L of TOOS (N-vinyl-N-(2-hydroxyl-3-sulfopropyl)-3-sodium methylaniline), and a solvent is purified water; the reagent R2 comprises the following components of 100mmol / L of Good's buffer solution, 5000U / L of POD (peroxidase), and 4mmol / L of 4-ampyrone, and a solvent is purified water. The kit is suitable for the laboratory requirements on the testing of the content of the small and dense low-density lipoproteincholesterin.

Description

technical field [0001] The invention belongs to the technical field of serum detection reagents, in particular to a small and dense low-density lipoprotein cholesterol detection kit. Background technique [0002] Lipoproteins in blood can be divided into four categories according to their density: high density lipoprotein (HDL), low density lipoprotein (LDL), very low density lipoprotein (VLDL) and chylomicrons (CM). Different types of lipoprotein cholesterol have different abilities to cause atherosclerosis. Usually the most clinically detected components are high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C). Conventionally, high-density lipoprotein cholesterol has a certain protective effect on arterial blood vessels. The higher the content, the lower the risk of cardiovascular disease, and the low-density lipoprotein cholesterol is easy to cause atherosclerosis and increase the risk of cardiovascular disease. However, recent st...

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Problems solved by technology

However, the steps of electrophoresis determination are cumbersome, and the precision and accuracy need to be improved, so the clinical applicability is not strong; the ultracentrifugation method is the standard method for the determination of lipoproteins, but the detection of sdLDL by this method requires an ultracentrifuge, and the detection is generally The amount is not high, and the requirements for operators are high, so this method has not been well promoted in clinical practice; although the fractional precipitation method makes the operation relatively simple, it still needs a precipitation step. The precision and accuracy of this method are relatively poor and not suitable for clinical application