A method for compound biological enzymatic hydrolysis to extract active components in kudzu root

Abstract

The invention relates to a method for compound biological enzymatic hydrolysis to extract active ingredients in Pueraria lobata, the steps are as follows: (1) After mixing water and Pueraria lobata powder, stir evenly, adjust the pH, then add an enzymolysis preparation once, and undergo enzymolysis to obtain Primary enzymatic hydrolysis solution; (2) adjust the pH value of the primary enzymatic hydrolysis solution, then add the secondary enzymatic hydrolysis preparation, and obtain the secondary enzymatic hydrolysis solution through enzymolysis; (3) separate the secondary enzymatic hydrolysis solution through solid-liquid separation , take the liquid, remove the water, and obtain the active ingredient of kudzu root. The present invention adopts the method of two-step enzymatic hydrolysis of Pueraria root with complex enzymes. Through the synergistic effect of different types of enzymes, the total isoflavone content of the process is increased by nearly 40% compared with the water extract of Pueraria root powder, and the puerarin content is compared with that of Pueraria powder. The water extract of Pueraria root powder has increased by 19.8%, and the amino acid content has increased by 4.3 times compared with the water extract of Pueraria root powder.

Description

A kind of method for compound biological enzymolysis to extract active components in pueraria technical field The present invention relates to a kind of method for compound biological enzymolysis to extract active ingredient in pueraria, belong to the field of plant extraction technology area. Background technique Pueraria lobata, as a kind of medicine and food homologous raw material, not only contains nutrients such as carbohydrates, lipids that humans take from plants. It contains primary metabolites such as phenylpropanoids, quinones, flavonoids, tannins, terpenoids, steroids and its Secondary metabolites such as glycosides and alkaloids. Pueraria isoflavones are mainly used for the treatment of cardiovascular and cerebrovascular, which can reduce vascular resistance, Increase brain flow, enhance immunity, also have the effect of resisting free radicals and improving SOD activity in blood and brain tissue. Pueraria As the main active ingredient in Puerarin is...

AI Technical Summary

Problems solved by technology

[0007] The above technologies only focus on retaining the enzyme active substances in Pueraria lobata, but the content of puerarin, total isoflavones, and free amino acids, the main active substances in Pueraria lobata, is not significantly improved

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