Glabridin preparation method, glabridin prepared according to glabridin preparation method and cosmetics

A technology of glabridin and licorice glabra residue, applied in cosmetics, cosmetic preparations, dressing preparations, etc., can solve the problems of high cost, cumbersome preparation process, environmental pollution, etc., and achieve low cost and simple process route Effect

Inactive Publication Date: 2018-12-25
抚顺天润生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patents CN102250107A, CN103360404A, CN105777771A, CN105294722A and CN104725394A disclose preparation methods of glabridin, but all use environmentally unfriendly organic solvents, such as ethyl acetate, etc., which easily cause environmental pollution and are not suitable for industrial production
Patent CN107383039 discloses the preparation method of glabridin. Although the preparation process does not include environmentally unfriendly organic solvents, the preparation process is cumbersome and requires three polyamide columns and one silica gel column. The cost is too high and it is not suitable for large-scale Production

Method used

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  • Glabridin preparation method, glabridin prepared according to glabridin preparation method and cosmetics
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  • Glabridin preparation method, glabridin prepared according to glabridin preparation method and cosmetics

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Experimental program
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Embodiment 1

[0113] The preparation of embodiment 1 glabridin

[0114] A preparation method of glabridin, comprising the following steps:

[0115] (1) Take 1kg of crushed Glycyrrhizae glabra, and use 10 parts by weight of water, 8 parts by weight of water and 8 parts by weight of water to extract three times at 80°C, each time for 60min; Licorice residue.

[0116] (2) Get the licorice glabra residue obtained in step (1), and use 10 parts by weight of 95% v / v ethanol, 8 parts by weight of 95% v / v ethanol and 8 parts by weight of 95% v / v ethanol at 70°C Extract three times, each time for 2h, filter, and combine to obtain the alcohol extract.

[0117] (3) Take the alcohol extract obtained in step (2), concentrate under reduced pressure and recover ethanol to obtain 110 g of extract A, add 2 L of 55% v / v ethanol, perform ultrasonic dissolution, filter to obtain the liquid part, and concentrate under reduced pressure to obtain alcohol extraction things.

[0118] (4) Macroporous adsorption r...

Embodiment 2

[0126] The dosage of Glycyrrhiza glabra in Example 1 was increased by 50 times to 50 kg, and the dosage of other raw materials was increased by corresponding ratios to finally obtain 14 g of glabradin with a purity of 97.8%.

[0127] After the large-scale production of the preparation method of the present invention, the prepared glabridin still has high purity.

Embodiment 3～15

[0129] The difference between Examples 3-15 and Example 1 lies in the specific process of alcohol extraction in step (2), the specific differences and the weight and purity of the finally prepared glabridin are shown in Table 3.

[0130] Table 3 embodiment 3~15 is different from the concrete process of the alcohol extraction of embodiment 1

[0131]

[0132] In Table 3, "—" represents the same as Example 1.

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Abstract

The invention belongs to the technical field of separation and purification and provides a glabridin preparation method, glabridin prepared according to the glabridin preparation method and cosmetics.The glabridin preparation method includes: performing alcohol extraction on glycyrrhiza glabra residues obtained by aqueous extraction to obtain alcohol extract liquid, and concentrating to obtain alcohol extract; then, sequentially adopting macroporous resin and polyamide for column chromatography separation to obtain eluent containing glabridin, and recrystallizing to obtain glabridin. By alcohol extraction of glycyrrhiza glabra residues subjected to aqueous extraction, more of water-soluble impurities in glycyrrhiza glabra are removed before alcohol extraction, high-purity glabridin is separated out through twice specific column chromatography separation, high-purity glabridin is finally obtained through recrystallization, and consumption of environment-unfriendly solvents in a preparation process is avoided. High-purity glabridin is prepared through alcohol extraction, twice column chromatography and recrystallization, and simple process route, low cost and suitableness for industrial production are realized.

Description

technical field [0001] The invention belongs to the technical field of separation and purification, and in particular relates to a preparation method of glabridin and glabridin and cosmetics obtained by the preparation method. Background technique [0002] Glabridin is one of the main flavonoids in Glycyrrhiza glabra L. Modern pharmacological studies have shown that glabridin has the functions of regulating immunity, lowering blood fat, lowering blood sugar, estrogen-like, antibacterial, anti-oxidation, anti-inflammatory inflammation etc. Glabridin shows a strong anti-free radical oxidation effect in the cytochrome P450 / NADPH oxidation system, which can significantly inhibit the free radicals produced in the metabolic process in the body, so as to prevent oxidation-sensitive biological macromolecules (low-density lipoprotein LDL, DNA) and cell walls are oxidized and damaged by free radicals, which can prevent certain pathological changes related to free radical oxidation, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04A61K8/49A61Q19/00A61Q19/02
CPCA61K8/498A61Q19/00A61Q19/02C07D493/04
Inventor 不公告发明人
Owner 抚顺天润生物技术有限公司
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