Physical cell disrupting microstructure device and cell disrupting and processing method thereof

Abstract

The invention discloses a physical cell disrupting microstructure device which structurally comprises a nitrogen input pipeline, a cell suspension solution input pipeline, a disrupting chamber and a disrupting plate. The disrupting chamber and a runner connection with the disrupting chamber are processed by an organic polymer molding method, the disrupting chamber is sealed by a thermal bonding method, and other portions are connected in a welded manner. A cell disrupting system ruptures cells by a physical collision method, and target components in the cells are extracted, so that next experiment is implemented. Cell suspension solution passes a pipeline outlet by a spray shape, and the diameter of spray particles is about 5 micrometers. The flow speed of nitrogen carried at high speed is200-300m / s, and the spray-shaped macro-particles are conveyed into the disrupting chamber and impact the disrupting plate at high speed, so that the cells are disrupted. Cell residue is collected inthe disrupting chamber and outputted from an outlet, and a follow-up micro-fluidic control experiment is implemented.

Description

technical field [0001] The invention relates to a microstructure device for physical cell crushing and a cell crushing and processing method thereof, belonging to the field of cell crushing. Background technique [0002] Microfluidic chip (microfluidic chip) is currently a hot field in the development of Miniaturized Total Analysis Systems. Its goal is to integrate the functions of the entire laboratory, including sampling, dilution, reagent addition, reaction, separation, detection, etc. There has been no systematically feasible cell disruption solution for microfluidic chips. Common cell disruption techniques can be divided into mechanical and non-mechanical methods. Mechanical methods include: [0003] High-pressure homogenization (homogenization), oscillating bead crushing (Skaking Bead), high-speed stirring bead grinding and crushing (fine grinding), ultrasonic crushing (ultrasonication); non-mechanical methods include: osmotic shock crushing (osmotic shock) ), Freez...

AI Technical Summary

Problems solved by technology

[0005] 1. The crushing system itself is too large to be combined with the microfluidic system

[0006] 2. Introduce chemical impurities, contaminate cell fragments, and affect the follow-up operation of the experiment

[0007] 3. The degree of cell fragmentation cannot be adjusted, which may cause excessive or insufficient fragmentation of cells

[0008] 4. The broken structure is complex and difficult to process

Images