Anti-aging stem cell composition and application thereof
A stem cell and composition technology, applied in the field of biomedicine, can solve problems such as difficulty in developing treatment methods, and achieve the effect of speeding up the recovery of the body and delaying the aging process
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] Embodiment 1 prepares HANK cell
[0030] Use the HANK cell in vitro culture kit of Shenzhen Hanke Bioengineering Co., Ltd. to prepare HANK cells. The basic operation steps are as follows:
[0031] (1) Collect peripheral anticoagulant blood from healthy people, and separate PBMC with lymphocyte separation medium;
[0032] (2) Culturing PBMCs in serum-free medium supplemented with IL-2 and membrane chimeric active factor complexes and X-Vivo15 can cause massive expansion and activation of NK cells;
[0033] (3) It can be used when cultured for about 14 days.
[0034] The NK cells expanded and activated in vitro are HANK cells. The number of cells is about 10 billion, the purity of NK cells is above 80%, and the killing rate of K562 target cells (effect-to-target ratio 10:1) is greater than 80%. It can be used fresh or frozen in liquid nitrogen for later use.
Embodiment 2
[0035] Example 2: Preparation of umbilical cord blood stem cells (hCBSC)
[0036] (1) Cord blood collection and isolation of mononuclear cells: Use a cord blood collection bag to collect about 100ml of cord blood, including 20ml of anticoagulant, sterilize the cord blood collection bag with 75% alcohol and put the collection bag into a biological In the safety cabinet, divide the cord blood into four 50ml centrifuge tubes with 15ml of lymphocyte separation solution added in advance, centrifuge at 2000rpm for 20min, after centrifugation, gently take out the centrifuge tube, at this time the centrifuge tube is divided into four layers, From bottom to top: erythrocyte layer, separation liquid layer, mononuclear cell layer, and plasma layer; collect the mononuclear cell layer into two 50ml centrifuge tubes, then fill up with normal saline, centrifuge at 1600rpm for 10min, and discard; After the precipitated cells are mixed, fill with normal saline, centrifuge at 1200rpm for 10min,...
Embodiment 3
[0039] Example 3: Preparation of human umbilical cord mesenchymal stem cells (hUC-MSC)
[0040] (1) The umbilical cord is taken from a healthy pregnant woman with a full-term cesarean section, and the tests of HBV antigen, anti-HCV antibody, anti-HIV antibody, anti-Treponema pallidum antibody, mycoplasma, and anti-cytomegalovirus antibody are all negative. TORCH, Down's screening, etc. were all normal;
[0041] (2) Collect the umbilical cord under sterile conditions, cut the umbilical cord into small pieces, about 1 cm each, remove the arteries and veins (two arteries and one vein), cut them into pieces in glass bottles, and put them in 15ml centrifuge tubes (each 2 sections in the tube, about 2cm), add collagenase digestion (type IV) 8ml, digest with 100 mesh filter after 3 hours (or centrifuge at 500rpm for 3-5 minutes), discard undigested tissue pieces, and pipette the supernatant into 15ml centrifuge tubes, about 5ml per tube, add PBS to 10ml, high-speed centrifugation (3...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More