Preparation method of conductive partitioned polyacrylonitrile spinal catheter

A polyacrylonitrile, partitioned technology, applied in the field of medical materials, can solve problems such as unsatisfactory spinal cord repair results and differences in histological structure, and achieve the effect of good electrical conductivity and high biocompatibility

Inactive Publication Date: 2019-01-04
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The idea of ​​initial nerve scaffold repairing spinal cord injury comes from the mature peripheral nerve repair, but the difference in histological structure makes the effect of spinal cord repair unsatisfactory

Method used

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  • Preparation method of conductive partitioned polyacrylonitrile spinal catheter

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the present embodiment comprises the following steps:

[0025] Weigh 0.5 g of terephthalic acid (PTA) powder and mix it into 10 mL of DMF, and stir at room temperature for 10 min until completely dissolved. Weigh 1 g of polyacrylonitrile (PAN) powder and dissolve it in the solution, heat and stir at 80 °C for 3 h to obtain a slightly yellow polyacrylonitrile spinning solution. The above solution was spun into nanofibers by electrospinning technique, such as figure 1 . The specific parameters are: the voltage is 12 kV, the spinning vessel used is a 10 mL plastic syringe with a vacuum diameter of 20 mm, the flow rate is 0.08 mm / min, the receiving distance is 15 cm, and the spinning time is 10 h. The polyacrylonitrile nanofibers deposited on the collector were placed in an oven at 60 °C for 24 h.

[0026] Disperse 2 g of the above-mentioned polyacrylonitrile nanofibers in 50 mL of deionized water, and stir at a high speed of 18000 r / min for 10 min to obtai...

Embodiment 2

[0028] Embodiment 2, this embodiment comprises the following steps:

[0029] Weigh 0.3 g of terephthalic acid (PTA) powder and mix it into 10 mL of DMF, and stir at room temperature for 10 min until completely dissolved. Weighed 1.2 g polyacrylonitrile (PAN) powder and dissolved it in the solution, heated and stirred at 70 °C for 3 h to obtain a slightly yellow polyacrylonitrile spinning solution. The above solution was spun into nanofibers by electrospinning technology, and the specific parameters were as follows: the voltage was 10 kV, the spinning vessel used was a 10 mL plastic syringe with a vacuum diameter of 20 mm, the flow rate was 0.05 mm / min, and the receiving distance was 10 cm. The spinning time was 8 h. The polyacrylonitrile nanofibers deposited on the collector were placed in an oven at 60 °C for 24 h.

[0030] Disperse 3 g of the above-mentioned polyacrylonitrile nanofibers in 50 mL of deionized water, and stir at a high speed of 18000 r / min for 30 min to obta...

Embodiment 3

[0032] Embodiment 3, this embodiment comprises the following steps:

[0033] Weigh 0.5 g of terephthalic acid (PTA) powder and mix it into 10 mL of DMF, and stir at room temperature for 10 min until completely dissolved. Weighed 1.2 g of polyacrylonitrile (PAN) powder and mixed it into 10 mL of DMF, heated and stirred at 80 °C for 3 h to obtain a slightly yellow polyacrylonitrile spinning solution. The above solution was spun into nanofibers by electrospinning technology. The specific parameters were as follows: the voltage was 15 kV, the spinning vessel used was a 10 mL plastic syringe with a vacuum diameter of 20 mm, the flow rate was 0.10 mm / min, and the receiving distance was 20 cm. The spinning time was 8 h. The polyacrylonitrile nanofibers deposited on the collector were placed in an oven at 60 °C for 24 h.

[0034] Disperse 2 g of the above-mentioned polyacrylonitrile nanofibers in 50 mL of deionized water, and stir at a high speed of 21000 r / min for 30 min to obtain ...

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Abstract

The invention discloses a preparation method of a conductive partitioned polyacrylonitrile spinal catheter. Polyacrylonitrile is selected as a raw material, the conductive partitioned spinal catheterof which the macroscopic configuration is matched with a spinal tissue structure and the microstructure is ordered and porous by an oriented refrigeration technology in combination with high-temperature carbonization; and exogenous electrical stimulation is used as physical factors and specific biological active factors which cooperatively regulate and control directional differentiation of composite neural stem cells, and NSCs is differentiated into functional cells which correspond to gray substance spinal cord and substantia alba medullae spinalis in different partitions in the same spinalcatheter. Influences of preparation parameters to the microstructure and the conductive property of the spinal catheter are researched; correlation effects of the microstructure of the catheter and cell adhesion and transfer are discovered; and physical/biological active factors are researched and the differentiation law of NSCs in the spinal catheter is regulated and controlled, and experiment basis and theoretical directions are constructed and provided for the design of a tissue engineered nerve stent.

Description

technical field [0001] The invention belongs to the field of medical materials, and in particular relates to a preparation method of a conductive partitioned polyacrylonitrile spinal cord catheter. technical background [0002] With the development of socioeconomic level, the incidence of spinal cord injury is increasing year by year. Improving the local regenerative microenvironment to effectively support nerve regeneration has become one of the key strategies in the current research on spinal cord injury repair. Autologous or allogeneic transplantation can repair some functions of the injured spinal cord, but the effect is not satisfactory; tissue-engineered nerve scaffolds provide a new idea for repairing spinal cord injuries. An ideal nerve scaffold can build a bridge for nerve stumps and provide a suitable microenvironment, providing bionic structural support and guiding information for axon regeneration and neural structure remodeling. The idea of ​​repairing spinal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/16A61L27/50A61L27/56D01F9/22B33Y70/00
CPCA61L27/16A61L27/50A61L27/56A61L2430/32B33Y70/00D01F9/22C08L33/20
Inventor 陈岑白雪阮世超郝亚倪俊杰
Owner ZHEJIANG SCI-TECH UNIV
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