Method for culturing bifidobacterium in common incubator, special culture medium and corresponding count method

A bifidobacteria and incubator technology, applied in the field of microorganisms, can solve the problems of difficulty in ensuring the stability of culture batches, cumbersome operation, and large investment, and achieve the effects of reduced capital, high operating skills, and simple operation.

Inactive Publication Date: 2019-01-04
THANKCOME BIOLOGICAL SCI & TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The use of anaerobic culture technology to cultivate bifidobacteria not only requires a lot of investment in equipment and chemicals, but also is cumbersome and requires operators to have high operational skills.
Due to the different equipment and personnel skills, it is difficult to guarantee the stability of the training batch

Method used

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  • Method for culturing bifidobacterium in common incubator, special culture medium and corresponding count method
  • Method for culturing bifidobacterium in common incubator, special culture medium and corresponding count method

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Embodiment Construction

[0039] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0040] 1. Preparation of culture medium and dilution solution

[0041] 1. Prepare the bottom medium

[0042] MRS-lactose solid medium: lactose 20g, peptone 10g, beef extract 10g, yeast powder 5g, anhydrous sodium acetate 5g, Tween-80 1ml, diammonium hydrogen citrate 2g, dipotassium hydrogen phosphate 2g, magnesium sulfate heptahydrate 0.58g, 0.19g manganese sulfate monohydrate, 1g L-cysteine ​​hydrochloride, 10g agar, add 1000ml water, pH 6.2-6.5, put them into Erlenmeyer flasks, and sterilize at 115°C for 20min.

[0043] The formula of this medium is obtained by optimizing carbon source and trace elements on the basis of MRS medium. Carbon source selection refers to the fermentation tests on various carbon sources of Bifidobacteria in the 8th edition of Bergey's Handbook. Comparing the MRS-lactose medium, MRS medium, BMD medium, BS me...

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Abstract

The invention provides a method for culturing bifidobacterium in a common incubator, a special culture medium and a corresponding count method. The special culture medium comprises an MRS-lactose solid medium and a pectin solid medium, wherein the MRS-lactose solid medium is laid on a panel bottom layer and mixed with a bacterial solution, the pectin solid medium is laid on the MRS-lactose solid medium, the panel is then inverted, and placed in the thermostatic incubator with the temperature of 37 DEG C for bacterial culturing. The special culture medium is laid by two layers, which makes theculturing of bifidobacterium in the common thermostatic incubator possible; the method for culturing bifidobacterium in a common incubator can be implemented through the common thermostatic incubator,without anaerobic incubators, anaerobic culture tanks, hungate roll-tubes and the like, and thus, investment input for equipment and medicament is low, operation is simple, and even general laboratory technicians can complete the operation after simple training.

Description

technical field [0001] The invention relates to a method for cultivating bacteria, a special culture medium and a corresponding counting method, in particular to a method for cultivating bifidobacteria in an ordinary incubator, a special culture medium and a corresponding counting method, belonging to the technical field of microorganisms . Background technique [0002] Bifidobacterium (bifidobacterium) is an important physiological bacteria in the intestinal tract of humans and animals, and its existence directly affects the health of the digestive tract. Bifidobacteria, as the main native bacteria, directly participate in the host's digestion, nutrition, metabolism, absorption, immunity and anti-infection processes. [0003] With the continuous deepening of the research on the biological characteristics and microecological significance of bifidobacteria, a large number of microecological preparations of bifidobacteria have emerged. The probiotic preparations prepared usin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/06C12R1/01
CPCC12N1/20C12Q1/045C12Q1/06G01N2333/195
Inventor 文学
Owner THANKCOME BIOLOGICAL SCI & TECH CO LTD
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