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Preparation method and application of spirulina phycocyanin

The technology of phycocyanin and spirulina is applied in the field of preparation of spirulina phycocyanin, which can solve the problems of complex extraction process and high cost, and achieve the effects of saving extraction time, saving cost and improving extraction rate.

Active Publication Date: 2019-01-08
广东一家人食品有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional freeze-thaw extraction process of spirulina phycocyanin is complex and costly

Method used

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  • Preparation method and application of spirulina phycocyanin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Accurately weigh 20 g of dried and pulverized spirulina powder, add phosphate buffer saline prepared by 0.05 mol / L phosphate and 0.004 mol / L sodium azide according to the volume ratio of material to liquid 1:15 g / ml, and mix well; Swell at 4°C for 4 hours, cut with an ultra-fine shearer for 10 minutes, centrifuge the cut material, and take the supernatant; slowly add ammonium sulfate to the supernatant until its saturation is 10%, remove For other protein impurities, centrifuge and take the supernatant; continue to slowly add ammonium sulfate solution to the supernatant until its saturation is 40%, centrifuge to obtain a blue precipitate; freeze-dry the precipitate for 18 hours to obtain spirulina Protein, phycocyanin yield was 9.23%.

Embodiment 2

[0025] Accurately weigh 20 g of dried and pulverized spirulina powder, add phosphate buffer (concentration is 0.15 mol / L, containing 0.004 mol / L sodium azide) according to the volume ratio of material to liquid 1:30 g / ml, and mix well; Swell at 4°C for 8 hours, cut for 20 minutes with an ultra-fine shearer, centrifuge the cut material, and take the supernatant; slowly add ammonium sulfate to the supernatant until its saturation is 20%, Remove other protein impurities, centrifuge, and take the supernatant; continue to slowly add ammonium sulfate solution to the supernatant until its saturation is 50%, and centrifuge to obtain a blue precipitate; freeze-dry the precipitate for 24 hours to obtain algae The yield of cyanocyanin and phycocyanin is 10.08%.

Embodiment 3

[0027] Accurately weigh 20 g of dried and pulverized spirulina powder, add phosphate buffer (concentration is 0.15 mol / L, containing 0.004 mol / L sodium azide) according to the volume ratio of material to liquid 1:25 g / ml, and mix well; Swell at 4°C for 6 hours, cut for 10 minutes with an ultra-fine shearer, centrifuge the cut material, and take the supernatant; slowly add ammonium sulfate to the supernatant until its saturation is 30%, Remove other protein impurities, centrifuge, and take the supernatant; continue to slowly add ammonium sulfate solution to the supernatant until its saturation is 60%, centrifuge, and take the blue precipitate; freeze-dry the precipitate in vacuum at -40°C for 24 Hour, obtain phycocyanin, and phycocyanin yield is 9.83%.

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Abstract

The invention belongs to a separation and extraction technology for an effective component of a natural product in light industry and chemical industry biotechnologies in biology and new medicine, andrelates to the field of extraction and application, in particular to an extraction method and application of spirulina phycocyanin. The preparation method comprises the following steps: (1) smashingand screening dried spirulina; (2) adding a phosphate buffer solution into the spirulina powder, and uniformly mixing the solution and the powder; (3) putting the slurry into an environment at 4 DEG Cfor swelling, and carrying out ultrafine shearing on the swollen slurry; (4) centrifugating an extracting solution to obtain supernate; (5) slowly adding ammonium sulfate into the supernate, and stewing and centrifugating the mixture to obtain supernate; (6) continuously slowly adding ammonium sulfate into the supernate, and stewing and centrifugating the mixture to obtain blue precipitates; and(7) carrying out freeze drying on the precipitates to obtain the phycocyanin. By the adoption of a swelling-ultrafine shearing combined wall-breaking technology, the method is short in time and high in phycocyanin yield; and the prepared spirulina phycocyanin extract has an obvious inhibiting effect on multiplication of HeLa cells.

Description

technical field [0001] The invention belongs to the separation and extraction technology of natural product active ingredients of light industry and chemical biotechnology in biology and new medicine, relates to the field of extraction and application, in particular to a preparation method and application of spirulina phycocyanin. Background technique [0002] Spirulina is a microalgae plant that grows in tropical and subtropical alkaline lakes. It is rich in various nutrients and active ingredients. It is called "the most ideal natural health food for human beings in the 21st century" by the Food and Agriculture Organization of the United Nations. With the improvement of living standards, people's concept of health and health preservation is gradually enhanced, and the demand for nutrition and health care products is also gradually increasing. Spirulina health care products have great market potential. Spirulina contains a large amount of protein and various nutrients, amon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/795C07K1/14C07K1/30
CPCC07K14/795
Inventor 黄旭民
Owner 广东一家人食品有限公司
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