HER2 gene fluorescent in-situ hybridization probe as well as preparation method and application thereof
A fluorescence in situ hybridization and probe technology, applied in biochemical equipment and methods, DNA preparation, DNA/RNA fragments, etc., can solve the problem of uncertain number of fluorescent probes, low probe specificity, and lack of small fragmentation etc. to achieve the effects of reducing background interference, increasing hybridization efficiency, and reducing hybridization time
Active Publication Date: 2019-01-08
广州简册生物技术有限公司
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The invention discloses an HER2 gene fluorescent in-situ hybridization probe as well as a preparation method and application thereof. The preparation method of the HER2 gene fluorescent in-situ hybridization probe as well as the HER2 gene fluorescent in-situ hybridization probe prepared with the preparation method are specially designed for a genome non-repeated region, so that non-specific reaction can be reduced and background interference can be reduced; the constructed probe is single-chain DNA, and can reduce pairing of the probe and the own and improve the hybridization efficiency compared with the traditional double-chain probe such as BAC or PCR products; furthermore, the constructed single-chain DNA probe has a small segment, can be combined with a targeted sequence rapidly and can reduce the hybridization time.
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Classification and recommendation of technical efficacy words
- Reduce non-specific reactions
- Low background noise
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