Reagent kit for assessing endometrial receptivity and using method of reagent kit

A technology of endometrium and reagent kit, which is applied in the field of medical and biological detection, can solve the problems that morphological evaluation cannot accurately reflect the expression of endometrial cells and cell factors, and validity disputes, and achieve high-efficiency endometrial content Acceptability, efficient evaluation, and good stability

Pending Publication Date: 2020-08-25
深圳市锦欣医疗科技创新中心有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, morphological assessment cannot accurately reflect the expression of endometrial cells and cellular factors, so the validity of its assessment is somewhat controversial in clinical practice

Method used

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  • Reagent kit for assessing endometrial receptivity and using method of reagent kit
  • Reagent kit for assessing endometrial receptivity and using method of reagent kit
  • Reagent kit for assessing endometrial receptivity and using method of reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Such as figure 1, the kit for evaluating endometrial receptivity in this embodiment, the kit includes a box body 1 and a first drawer-type compartment 2, a second drawer-type compartment 3 and a second drawer compartment arranged in the box body 1 Three drawer compartments 4, the first drawer compartment 1 is provided with a first foam cushion 801, the second drawer compartment 3 is provided with a second foam cushion 802, and the first foam cushion 801 Open 6 grooves that are respectively placed with gDNA scavenger buffer 9, gDNA scavenger 10, cDNA reverse transcription buffer 11, cDNA reverse transcriptase mixture 12, reverse transcription primer mixture 13 and the first enzyme-free water 14, said The first foam pad 801 offers a cavity for placing a 96-orifice plate 15 for reverse transcription, and the second foam pad 802 offers 7 grooves for placing probes 16, 1 groove for a positive control standard, and real-time Fluorescent quantitative polymerase chain reaction...

example

[0069] Example: By implementing fluorescent quantitative polymerase chain reaction, the Ct values ​​of the positive control standard and the sample to be tested are obtained.

[0070]

[0071] By △△C T Calculate the relative expression of the target gene using the method (relative expression = 2 -△△Ct ):

[0072]

[0073] Correct the specific expression level by ActB and B2M, and take the mean of the two:

[0074]

[0075] That is, the relative expression levels of SLC2A1, HK2, PKM2, HIF1A, and SLC16A3 in the endometrium of patient 1 were 0.9709, 1.5306, 1.3419, 0.7378, and 1.1465 after correction. After correcting the data of SLC2A1, HK2, PKM2, HIF1A, and SLC16A3 in the endometrium of patient 2, the relative expression levels were 0.2630, 0.1914, 0.4690, 0.4729, and 0.3942, respectively.

[0076] Compared with the cut-off values ​​of SLC2A1, HK2, PKM2, HIF1A, and SLC16A3 (0.5534; 0.6443; 0.6903; 0.7111; 0.6235), the relative expression levels of patient 1 were hig...

Embodiment 2

[0078] Such as figure 2 , the kit includes a box body (1) and a first drawer compartment (2), a second drawer compartment (3) and a third drawer compartment (4) arranged in the box body (1) ,

[0079] A first foam pad (801) is provided in the first drawer compartment (1), and a second foam pad (802) is provided in the second drawer compartment (3),

[0080] The first foam pad (801) has 6 sets of gDNA scavenger buffer (9), gDNA scavenger (10), cDNA reverse transcription buffer (11), cDNA reverse transcriptase mixture (12), reverse Record the groove of the primer mixture (13) and the first enzyme-free water (14), and the first foam pad (801) offers a cavity for placing the reverse transcription reaction using a 96-well plate (15),

[0081] The second foam pad (802) is provided with 7 grooves for placing the probe (16), 1 groove for placing the positive standard (17), and 1 groove for placing the real-time fluorescent quantitative polymerase chain reaction solution (19). Groo...

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Abstract

The invention relates to a reagent kit for assessing endometrial receptivity. The reagent kit comprises gDNA scavenger buffer liquid, a gDNA scavenger, cDNA reverse transcription buffer liquid, a cDNAreverse transcription mixture, a reverse transcription primer mixture, real-time fluorescent quantitation polymerase chain reaction liquid, a probe, a positive control standard product and enzyme-free water. The probe is combined with a target gene through molecular hybridization to generate hybridized signals, so that the target gene in massive genomes can be shown. A specific sequence of reverse transcription products is combined with detection of probe signals, so that expression level of specific genes can be detected, and the endometrial receptivity can be conjectured. The manner is simple to operate, high in sensitivity, high in specificity, accurate in detection results, objective and quick, so that a clinician can be helped to clear the state of the endometrial receptivity, and the reagent kit can provide a basis for early diagnosis, target intervention and guidance of an embryo transplanting opportunity for infertility patients.

Description

technical field [0001] The invention relates to the field of medical and biological detection, in particular to a kit for evaluating endometrium receptivity and a use method thereof. Background technique [0002] At present, the incidence of infertility in China is about 15-20%, and the number of infertile patients is increasing at a rate of hundreds of thousands every year. Factors such as delayed marriage and childbearing age, accumulation of work pressure, and changes in lifestyle have directly or indirectly reduced people's fertility. With the birth of the world's first test-tube baby, in vitro fertilization-embryo transfer (IVF-ET) has become an effective way for infertile women to obtain pregnancy as soon as possible. However, in the actual clinical practice, the low embryo implantation rate is still one of the problems that have plagued medical staff of assisted reproductive technology for a long time. Endometrial receptivity is a key factor in the process of embryo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6851
CPCC12Q1/6883C12Q1/6851C12Q2600/158C12Q2600/166C12Q2531/113C12Q2563/107C12Q2545/114C12Q2527/125
Inventor 刁梁辉蔡松辰黄春宇李玉叶李龙飞余姝毅陈聪曾勇
Owner 深圳市锦欣医疗科技创新中心有限公司
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