Method for establishing Malus sieversii regeneration system based on callus differentiation

A callus and apple technology is applied in the field of establishing a regeneration system of Xinjiang wild apple based on callus differentiation, which can solve the problems of low transformation efficiency and less research on tissue culture systems, and achieve vigorous growth, dark green color, and improved effect on survival

Inactive Publication Date: 2019-01-11
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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Problems solved by technology

However, little research has been done on the tissue culture system of the ancestral species of cultivated apple, Xinjiang Wild Apple.
Li Xinyue (2018), Liu Bing (2011), Tan Dongmei (2009) established the tissue culture system of Xinjiang wild apple with stem segments as explants, but the transformation efficiency was low; (2017) used the leaves of Xinjiang wild apple 31 as explants to directly induce adventitious buds, but this material is a "highl

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  • Method for establishing Malus sieversii regeneration system based on callus differentiation
  • Method for establishing Malus sieversii regeneration system based on callus differentiation
  • Method for establishing Malus sieversii regeneration system based on callus differentiation

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Embodiment 1

[0029] A kind of method that the Xinjiang wild apple regeneration system based on callus differentiation of the present invention establishes, and this method takes leaf as explant, the induction of callus tissue and adventitious bud, the multiplication of adventitious bud, rooting culture and refining Seedlings and transplanting, the specific operation is carried out according to the following steps:

[0030] Induction of callus and adventitious buds:

[0031] a. Take the young and fully expanded leaves of Xinjiang wild apple aseptic seedlings with a leaf age of 40 days grown in the laboratory as explants, remove the petiole, cut the leaves perpendicularly to the central vein of the leaves with a scalpel, and divide the leaves into equal parts The two parts, using the distal end of the leaf as the contact surface, inoculated into the induction medium for subculture twice, once every 25 days, and cultured in the dark for three weeks, cultured in the light of 2000lx, the light ...

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Abstract

The invention belongs to the technical field of plant tissue culture, and relates to a method for establishing a Malus sieversii regeneration system based on callus differentiation. The method is completed through the steps of induction of calluses and adventitious buds, proliferation of the adventitious bud, rooting culture, and hardening and transplantation of seedlings. The method allows the callus induction rate to be 100%, the highest induction rate of the adventitious buds to be 30%, the rooting rate to reacj 85% and the survival rate of the seedlings to reach 90%. The method provides abasis for the germplasm preservation and genetic transformation study of Malus sieversii. The method is suitable for various environments, especially Xinjiang, Gansu and other places with low air humidity, greatly improves the survival rate of tissue culture plants, and saves time and labor.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for establishing a Xinjiang wild apple regeneration system based on callus differentiation. Background technique [0002] Xinjiang wild apple (Malus sieversii), also known as Seves apple, belongs to the genus Malus and is the ancestor of cultivated apples in the world (Velasco et al.2010; Duan et al.2017). Xinjiang wild apple is a relict species of the Tertiary period, and its fruit shape, coloring, flavor, plant height, tree shape, etc. vary a lot. Because of its self-incompatibility and natural selection, it has formed many unique plants that are cold-resistant, disease-resistant, and poor-resistant. The rich variation has won widespread attention and has become the main rootstock in Northwest China and other provinces. one. The Tianshan wild fruit forest located in the Ili Valley of Xinjiang, with wild apples as the main species, is an impor...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张道远张燕刘晓洁李小双杨红兰
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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