Particle sorting device and method for sorting particles in liquid

Abstract

The invention discloses a particle sorting device based on acoustic surface waves. The particle sorting device comprises a piezoelectric substrate, a first interdigital transducer, a liquid storage area, flow guiding pipes, a liquid injection area, micro channels, two second interdigital transducers and a liquid discharging area, wherein the first interdigital transducer, the liquid storage area,the flow guiding pipes, the liquid injection area and the micro channels are arranged on the piezoelectric substrate; and the two second interdigital transducers are oppositely arranged on the two sides of the micro channels. The liquid storage area comprises a plurality of liquid storage grooves. The liquid injection area comprises a plurality of liquid injection grooves. The liquid discharging area comprises at least two liquid discharging grooves. The liquid storage grooves and the liquid injection grooves are connected through the flow guiding pipes. The liquid injection grooves and the liquid discharging grooves are connected through the micro channels. The first interdigital transducer generates row acoustic surface waves on the upper surface of the piezoelectric substrate and liquidin the liquid storage grooves is pushed to flow towards the liquid injection grooves, the micro channels and the liquid discharging area. The two second interdigital transducers generate two rows ofrow acoustic surface waves which are opposite in direction and have the same frequency and speed on the upper surface of the piezoelectric substrate, the two rows of the row acoustic surface waves areoverlapped to form standing wave acoustic surface waves, and thus the particles in the liquid of the micro channels can skew to corresponding pressure nodes of the standing wave acoustic surface waves. The problems that the particles cannot be separated conveniently and errors are large are solved.

Description

technical field [0001] The invention relates to a particle sorting device and a method for sorting particles in liquid. Background technique [0002] In the prior art, the method used for sorting micron-sized particles is usually an air flow particle sorting device, which has lower requirements for sorting accuracy, or adopts centrifugal method and micro-sieve method, which are often due to the particle diameter and separation The sorting diameter of the sorting equipment does not match, resulting in measurement errors. At the same time, as people pay more and more attention to their own health checks, blood routine testing is the most common blood testing in hospitals, but the testing of important parameters of some cells in the blood requires large-scale equipment as technical support, and these equipment are relatively expensive and bulky. Large and inconvenient to use, for example, a flow cytometer. The flow cytometer must be carefully adjusted before and during use. W...

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Problems solved by technology

[0002] In the prior art, the method used for sorting micron-sized particles is usually an air flow particle sorting device, which has lower requirements for sorting accuracy, or adopts centrifugal method and micro-sieve method, which are often due to the particle diameter and separation The separation diameter of the selection equipment does not match, resulting in measurement errors

At the same time, as people pay more and more attention to their own health checks, blood routine testing is the most common blood testing in hospitals, but the testing of important parameters of some cells in the blood requires large-scale equipment as technical support, and these equipment are relatively expensive and bulky. Large and inconvenient to use, e.g., flow cytometers

The flow cytometer must be carefully adjusted before and during use. While ensuring the stable working conditions of the photomultiplier tube, it is also necessary to pay attention to the magnetic shielding of the equipment to ensure the reliability and stability of the work, resulting in equipment operation Complicated, the cost of maintenance is huge, and it is not convenient for cell sorting

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