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A kind of SNP site for detecting variants of abo blood group

A technology of ABO blood type and mutation, applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., to achieve rapid detection and avoid the effect of acute hemolytic transfusion reaction

Active Publication Date: 2021-11-02
QINGDAO BLOOD CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As long as 5-10ml of ABO-incompatible blood is transfused, obvious symptoms will appear; blood transfusion exceeding 200ml will cause serious consequences

Method used

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  • A kind of SNP site for detecting variants of abo blood group
  • A kind of SNP site for detecting variants of abo blood group
  • A kind of SNP site for detecting variants of abo blood group

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Confirm the SNP site through genetic testing of the proband with ABO blood type variant.

[0027] 1. Extraction of peripheral blood genomic DNA:

[0028] In line with the relevant national policies and regulations, and on the basis of the consent of the sampling subjects, draw 2-5ml of peripheral venous blood from members of the ABO blood type variant gene family and put it into EDTA-Na 2 In anticoagulant tubes, freeze at -80°C for later use; DNA extraction uses the DNA extraction kit from LIFE Company, and the specific operation process is as follows:

[0029] After thawing the frozen EDTA anticoagulated blood at room temperature, take 500ul and put it in a centrifuge tube, add an equal volume of erythrocyte lysate (pH8.0), vortex and mix for 5 minutes, centrifuge at 12000rpm for 5 minutes, and discard the supernatant. Add 500ul of red blood cell lysate repeatedly, vortex and mix for 5 minutes, centrifuge at 12000rpm for 5 minutes, and discard the supernata...

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Abstract

The present invention provides a SNP site that causes variation in ABO blood type, which is the 526th, 703rd, 796th or 803rd base of the start codon in the coding region of the ABO blood type gene; wherein the 526th base is CC homozygous, The 703rd base is GG homozygous, the 796th base is CA heterozygous, and the 803rd base is GC heterozygous. The present invention provides a new application of ABO blood type variant gene detection, thereby providing an effective way to avoid the occurrence of acute hemolytic transfusion reactions in gene diagnosis, prenatal genetic screening and genetic counseling, and the application effect shows that the present invention provides The SNP site of the gene and the detection primer can be effectively used in the rapid detection of the ABO blood group variant gene mutation site in the peripheral blood of clinical patients.

Description

technical field [0001] The invention belongs to the technical field of gene diagnosis products, and specifically relates to a SNP site for detecting ABO blood type variants, that is, a SNP site that causes ABO blood type gene variation and triggers acute hemolytic transfusion reactions. [0002] Background of the invention [0003] Acute Hemolytic Transfusion Reaction (AHTR) usually occurs within 24 hours after blood transfusion, more often than immediately after blood transfusion. As long as 5-10ml of ABO-incompatible blood is transfused, obvious symptoms will appear; blood transfusion exceeding 200ml will cause serious consequences. Clinically, acute hemolytic transfusion reaction often manifests as intravascular hemolysis. Patients with fever, chills, nausea, and back pain, followed by hemoglobinuria, hemoglobinemia, and hypotensive shock, should be considered as acute intravascular hemolytic transfusion reaction. , the blood transfusion should be stopped quickly and vari...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 韩斌冯智慧
Owner QINGDAO BLOOD CENT