Method for preparing and using electrochemical immunosensor for aflatoxin B1 detection

An immune sensor and aflatoxin technology, applied to scientific instruments, instruments, measuring devices, etc., can solve the problems of high cost, poor effect, and great harm, and achieve short response time, increased specific surface area, and low detection cost Effect

Active Publication Date: 2019-01-11
CHENGDU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing and using an electrochemical immunosensor for the detection of aflatoxin B1, so as to solve the problems of high cost, poor effect and great harm in the preparation and use of common electrochemical immunosensors

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  • Method for preparing and using electrochemical immunosensor for aflatoxin B1 detection
  • Method for preparing and using electrochemical immunosensor for aflatoxin B1 detection

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Experimental program
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Effect test

Embodiment 1

[0029] 1. The preparation of the electrochemical immunosensor is as follows:

[0030] Prepare nano gold solution, then the concentration of 1 volume part A1 is the graphene oxide of 1.01wt%, the nano gold solution of the thionine of 1 weight part B1, 8.333 volume parts A1 is placed in band cap weighing bottle, 1 volume part The relationship between A1 and 1 part by weight of B1 is: 1 ml corresponds to 1.25 mg. A stirring magnet is placed in the weighing bottle. After the weighing bottle is capped, it is placed on a magnetic stirring table and stirred for about 4 hours to fully mix the materials in the bottle to obtain oxidation. Graphene / thionine / nano-gold composite material, stored at 4°C, drop 1 volume part of A2 graphene oxide / thionine / nano-gold composite material 2S on the surface of the activated glassy carbon electrode, and then place the glassy carbon electrode on Store in a refrigerator at 4°C for 12 hours until the surface of the glassy carbon electrode forms a solid ...

Embodiment 2

[0041] 1. The preparation of the electrochemical immunosensor is as follows:

[0042] Prepare nano-gold solution, then the concentration of 1 volume part A1 is the graphene oxide of 0.5wt%, the thionine of 1 weight part B1, the nano-gold solution of 5 volume parts A1 is placed in band cap weighing bottle, 1 volume part The relationship between A1 and 1 part by weight of B1 is: 1 milliliter corresponds to 1 milligram. A stirring magnet is placed in the weighing bottle. After the weighing bottle is capped, it is placed on a magnetic stirring table and stirred for about 4 hours to fully mix the materials in the bottle to obtain oxidation. Graphene / thionine / nano-gold composite material, stored at 2°C, drop 1 volume part of A2 graphene oxide / thionine / nano-gold composite material 1S on the surface of the activated glassy carbon electrode, and then place the glassy carbon electrode on Store in a refrigerator at 2°C for 10 hours until the surface of the glassy carbon electrode forms a...

Embodiment 3

[0053] 1. The preparation of the electrochemical immunosensor is as follows:

[0054] Prepare nano-gold solution, then the concentration of 1 volume part A1 is the graphene oxide of 1.5wt%, the thionine of 1 weight part B1, the nano-gold solution of 12 volume parts A1 is placed in band cap weighing bottle, 1 volume part The relationship between A1 and 1 part by weight of B1 is: 1 ml corresponds to 5 mg. A stirring magnet is placed in the weighing bottle. After the weighing bottle is capped, it is placed on a magnetic stirring table and stirred for about 4 hours to fully mix the materials in the bottle to obtain oxidation. Graphene / thionine / nano-gold composite material, stored at 8°C, 1 volume part of A2 graphene oxide / thionine / nano-gold composite material 3S was dropped on the surface of the activated glassy carbon electrode, and then the glassy carbon electrode was placed Store in a refrigerator at 8°C for 14 hours until the surface of the glassy carbon electrode forms a soli...

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Abstract

The invention discloses a method for preparing and using an electrochemical immunosensor for aflatoxin B1 detection, including preparation of an electrochemical immunosensors and alflatoxin B1 detection of the electrochemical immunosensor. According to the preparation scheme, the preparation method comprises the following steps: preparing a nano-gold solution; preparing an activated glassy carbonelectrode; preparing a graphene oxide/thionine/nano-gold compound material; preparing a modified glassy carbon electrode; sealing the non-specific site with bovine serum albumin; and preparing a detection substrate. The method realizes quick, accurate and low-cost detection on aflatoxin B1.

Description

technical field [0001] The invention relates to a method for preparing and using an electrochemical immunosensor, in particular to a method for preparing and using an electrochemical immunosensor for aflatoxin B1 detection. Background technique [0002] As a secondary metabolite of Aspergillus flavus and Aspergillus parasiticus, aflatoxin exists widely in grain, oil and food. It is the strongest type of mycotoxin that has been found to pollute agricultural products in nature. It is highly carcinogenic and pathogenic to humans and animals. A class of mycotoxins. Common aflatoxins are mainly divided into four types according to their chemical structures: B1, B2, G1, and G2. Among them, aflatoxin B1 is the most toxic, far exceeding the toxicity of arsenic and potassium cyanide. [0003] At present, the detection methods of aflatoxin mainly include ELISA, high performance liquid chromatography (HPLC), thin layer chromatography (TLC) and so on. Among them, the enzyme-linked imm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56961
Inventor 梅丽傅小红尚书勇徐坤张强淳丹陈林雷怡
Owner CHENGDU NORMAL UNIV
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