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Automatic-sexing breeding method for specialized strains of Huoyan male and female meat geese

A technology that separates male and female and matched goose lines is applied in the breeding field of Huoyan separate male and female meat goose matched lines. The effect of prevention and control

Inactive Publication Date: 2019-01-15
LIAONING ACAD OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a breeding method for self-separating male and female meat geese, which solves the problem of stress and damage to gooses caused by the one-day-old sex anus identification method in the current production of meat geese. problem, the breeding method of self-separating male and female goose mating lines does not cause any harm to goslings, and improves the survival rate of goslings. Its identification speed is fast, the accuracy is high, the cost is low, and it is beneficial to actual production and application

Method used

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  • Automatic-sexing breeding method for specialized strains of Huoyan male and female meat geese

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Embodiment

[0020] The breeding method of the male-female matching line with the character of Goose Huoyan, such as figure 1 As shown, it includes the following steps:

[0021] (1) Detection of FREM1 gene variation sites: observe the traits of Huoyan in the hybrid offspring of Huoyan female geese and large male geese, and perform PCR on all the female geese whose offspring are Huoyan and half-siblings are Huoyan geese -RFLP detection; the specific method is as follows: collect the blood of the goose to be tested and extract DNA, design primers according to the FREM1 gene variation site, perform PCR amplification, perform enzyme digestion according to HindIII restriction endonuclease, and perform agarose detection on the digested product , if only one band of about 180bp appears, it is determined that the goose to be tested is homozygous for the FREM1 gene mutation; if two bands of 180bp and 250bp appear, it is determined that the goose to be tested is heterozygous for the FREM1 gene mutat...

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Abstract

The invention discloses an automatic-sexing breeding method for the specialized strains of Huoyan male and female meat geese and belongs to the technical field of livestock breeding. The automatic-sexing breeding method for the specialized strain of Huoyan male and female meat geese comprises the steps of detecting a mutation site of the FREM1 gene, performing the expanding propagation of a mutantgroup, breeding the pure line of a male parent, and establishing an automatic-sexing male and female specialized strain system for the traits of Huoyan geese. According to the breeding method disclosed by the invention, a parent gene type and a hybridization process are designed by utilizing the associated partial loss traits of the upper eyelid parts of geese, so that male and female geese can be directly identified according to the phenotypes of the hybridization F1 generation in the cultivation process. The identification accuracy of the method can reach more than 90%. According to the invention, the breeding method is simple, convenient and easy to operate, and the special identification personnel do not need to be trained. Therefore, a large amount of cost spent in the manual anal turning operation for identifying male and female goslings can be saved. The method is fast in identification speed and high in accuracy. Any damage to goslings is greatly reduced. The survival rate ofgoslings is improved, and the prevention and control of diseases are facilitated.

Description

technical field [0001] The invention relates to a breeding method of Huoyan self-separating male and female meat goose matching lines, which belongs to the technical field of poultry breeding. Background technique [0002] China is a big country of goose production and consumption. With the continuous development and expansion of China's goose breeding industry, the problem of sex identification of day-old goslings is becoming more and more serious. The male and female are identified and processed separately according to their respective production purposes, so as to reduce feeding costs and improve economic benefits. [0003] There are few methods to identify male and female goslings, which can be generally divided into the following categories: 1. Body identification method. There are certain differences between male and female chicks when they hatch. Large, long and wide beak, long body, large head, long neck, and straight standing posture; the female chick is smaller, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02C12Q1/683
CPCA01K67/02C12Q1/683C12Q2531/113
Inventor 于金成赵辉李喆于宁杨姝
Owner LIAONING ACAD OF AGRI SCI