Automatic-sexing breeding method for specialized strains of Huoyan male and female meat geese
A technology that separates male and female and matched goose lines is applied in the breeding field of Huoyan separate male and female meat goose matched lines. The effect of prevention and control
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment
[0020] The breeding method of the male-female matching line with the character of Goose Huoyan, such as figure 1 As shown, it includes the following steps:
[0021] (1) Detection of FREM1 gene variation sites: observe the traits of Huoyan in the hybrid offspring of Huoyan female geese and large male geese, and perform PCR on all the female geese whose offspring are Huoyan and half-siblings are Huoyan geese -RFLP detection; the specific method is as follows: collect the blood of the goose to be tested and extract DNA, design primers according to the FREM1 gene variation site, perform PCR amplification, perform enzyme digestion according to HindIII restriction endonuclease, and perform agarose detection on the digested product , if only one band of about 180bp appears, it is determined that the goose to be tested is homozygous for the FREM1 gene mutation; if two bands of 180bp and 250bp appear, it is determined that the goose to be tested is heterozygous for the FREM1 gene mutat...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More 
