Preparing method of stropharia rugoso-annulata reduction strain and culture and application

A technology of stropharia stropharia and a production method, which is applied in the field of cultivation and edible fungus seed production, can solve the problems of large amount of solid cultivated seeds, low seeding efficiency, and large difference in bacterial age, and achieve complex operation, high cost, and large occupied volume , the effect of large differences in bacterial age

Inactive Publication Date: 2019-01-18
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in production, solid cultivars are usually used for inoculation and cultivation, which requires a large amount of solid cultivars and large differences in bacterial ages, resulting in low seeding efficiency
The more prominent problem is that the strains of Stropharia stropharii are relatively vigorous, and the mycelium of solid strains will generate a lot of heat due to the mixture of mycelium and solid substrate, resulting in the phenomenon of burning bacteria. The quality of mushroom strains and the demand for long-distance transportation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: A kind of production method and cultivation application of Stropharia stropharia reduced strain

[0019] Application of Liquid Cultivated Species of Stropharia grandis

[0020] (1) Preparation of the original species of Stropharia stropharia

[0021] Transfer the mother species of Stropharia grandis in the test tube to the PDA plate for activation, and continue the transfer and activation for 3 times until the mycelium vitality of the mother species recovers.

[0022] (2) Preparation of liquid first-class species of Stropharia stropharco

[0023] Add 100mL PDB liquid medium into a 250mL Erlenmeyer flask, and sterilize for 15min at a temperature of 121°C and a pressure of 0.15Mpa.

[0024] (3) After sterilizing and cooling, insert 6 pieces of activated Stropharia stropharia PDA bacteria blocks with a diameter of 1 cm, and place them in a shaker incubator at 23°C at a speed of 150 rpm for 7 days in the dark.

[0025] (4) The culture medium formula of Stro...

Embodiment 2

[0032] Embodiment 2: A kind of production method and cultivation application of Stropharia stropharia reduced strain

[0033] A method for making reduced bacterial strains of Stropharia stropharia, comprising the following steps:

[0034] The first step: transfer the mother species of Stropharia to a plate containing PDA medium for activation, so as to restore the mycelium vitality of the mother species of Stropharia, and then divide to obtain the original PDA strain of Stropharia Piece;

[0035] Step 2: Inoculate the PDA bacterial block of the original species of Stropharia stropharia into the sterilized PDB liquid medium, and culture it in the dark with a shaker incubator at 22°C for 7 days to obtain Stropharia stropharia first-class species;

[0036] Step 3: Inoculate the first-grade species of Stropharia stropharia into a fermenter containing a liquid culture medium of Stropharia stropharia, and then culture the inoculated fermenter at 22°C for 7 days; the stropharia Th...

Embodiment 3

[0044] Embodiment 3: A kind of production method and cultivation application of Stropharia stropharia reduced strain

[0045] A method for making reduced strains of Stropharia stropharia, comprising the following steps:

[0046] The first step: transfer the mother species of Stropharia to a plate containing PDA medium for activation, so as to restore the mycelium vitality of the mother species of Stropharia, and then divide to obtain the original PDA strain of Stropharia Piece;

[0047] Step 2: inoculate the PDA bacterial block of the original species of Stropharia stropharia into the sterilized PDB liquid medium, and culture it in a shaker incubator in the dark for 8 days at 26°C to obtain Stropharia stropharia first-class species;

[0048] Step 3: inoculate the first-grade species of Stropharia stropharia into a fermenter containing a liquid culture medium of Stropharia stropharia, and then cultivate the inoculated fermenter at 26°C for 8 days; the stropharia The preparat...

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Abstract

The invention discloses a preparing method of a stropharia rugoso-annulata reduction strain and culture and application. By means of the method, a traditional solid strain mode is broken through, thenew mode of preparing a liquid strain, separating the strain and a culture substrate, conveying the pure strain and adding liquid on purpose to reduce the strain is adopted, and meanwhile in cooperation with the liquid formula of the reduction strain, large-field or under-forest spraying inoculation can be conducted. In the liquid formula, 0.01-0.05% of yeast powder, 0.03-0.08% of K2HPO4, 0.03-0.08% of KH2PO4, 0.01-0.05% of MgSO4 and 0.1% of decavitamin solution are added to 1 L of distilled water, wherein the decavitamin solution is prepared by mixing 0.1% of thiamine hydrochloride and 0.01%of riboflavin and adding water. On one hand, the liquid culture seed sowing process is simple, the preparing cost is low, fungus generation is rapid, and the stropharia rugoso-annulata culture efficiency is remarkably improved; on the other hand, the separated pure strain is small in size, convenient to convey and low in heating amount, the strain is separated from the culture substrate, and the heating fungus burning in the strain conveying process is effectively avoided.

Description

technical field [0001] The invention relates to a production method and cultivation application of a reduced strain of Stropharia stropharcoides, belonging to the technical field of edible fungus seed production and cultivation. Background technique [0002] Large Stropharia ( Stropharia rugoso-annulata ), belonging to the order Agaricaceae, Strophariaceae, and the genus Stropharia. It is a new mushroom species recommended by the Food and Agriculture Organization of the United Nations (FAO) to developing countries, and it is also one of the top ten varieties in the international mushroom trading market. Its fruiting body contains a variety of essential amino acids and vitamins, and has medicinal value in preventing coronary heart disease, anti-tumor and other diseases, and is very popular among consumers. At present, in production, solid cultivars are usually used for inoculation and cultivation, which requires a large amount of solid cultivars and large differences in bact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/40A01G18/20A01G18/00
CPCA01G18/00A01G18/20A01G18/40
Inventor 陈辉张津京汪虹黄建春陈明杰王倩
Owner SHANGHAI ACAD OF AGRI SCI
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