Preparing method of stropharia rugoso-annulata reduction strain and culture and application
A technology of stropharia stropharia and a production method, which is applied in the field of cultivation and edible fungus seed production, can solve the problems of large amount of solid cultivated seeds, low seeding efficiency, and large difference in bacterial age, and achieve complex operation, high cost, and large occupied volume , the effect of large differences in bacterial age
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Embodiment 1
[0018] Embodiment 1: A kind of production method and cultivation application of Stropharia stropharia reduced strain
[0019] Application of Liquid Cultivated Species of Stropharia grandis
[0020] (1) Preparation of the original species of Stropharia stropharia
[0021] Transfer the mother species of Stropharia grandis in the test tube to the PDA plate for activation, and continue the transfer and activation for 3 times until the mycelium vitality of the mother species recovers.
[0022] (2) Preparation of liquid first-class species of Stropharia stropharco
[0023] Add 100mL PDB liquid medium into a 250mL Erlenmeyer flask, and sterilize for 15min at a temperature of 121°C and a pressure of 0.15Mpa.
[0024] (3) After sterilizing and cooling, insert 6 pieces of activated Stropharia stropharia PDA bacteria blocks with a diameter of 1 cm, and place them in a shaker incubator at 23°C at a speed of 150 rpm for 7 days in the dark.
[0025] (4) The culture medium formula of Stro...
Embodiment 2
[0032] Embodiment 2: A kind of production method and cultivation application of Stropharia stropharia reduced strain
[0033] A method for making reduced bacterial strains of Stropharia stropharia, comprising the following steps:
[0034] The first step: transfer the mother species of Stropharia to a plate containing PDA medium for activation, so as to restore the mycelium vitality of the mother species of Stropharia, and then divide to obtain the original PDA strain of Stropharia Piece;
[0035] Step 2: Inoculate the PDA bacterial block of the original species of Stropharia stropharia into the sterilized PDB liquid medium, and culture it in the dark with a shaker incubator at 22°C for 7 days to obtain Stropharia stropharia first-class species;
[0036] Step 3: Inoculate the first-grade species of Stropharia stropharia into a fermenter containing a liquid culture medium of Stropharia stropharia, and then culture the inoculated fermenter at 22°C for 7 days; the stropharia Th...
Embodiment 3
[0044] Embodiment 3: A kind of production method and cultivation application of Stropharia stropharia reduced strain
[0045] A method for making reduced strains of Stropharia stropharia, comprising the following steps:
[0046] The first step: transfer the mother species of Stropharia to a plate containing PDA medium for activation, so as to restore the mycelium vitality of the mother species of Stropharia, and then divide to obtain the original PDA strain of Stropharia Piece;
[0047] Step 2: inoculate the PDA bacterial block of the original species of Stropharia stropharia into the sterilized PDB liquid medium, and culture it in a shaker incubator in the dark for 8 days at 26°C to obtain Stropharia stropharia first-class species;
[0048] Step 3: inoculate the first-grade species of Stropharia stropharia into a fermenter containing a liquid culture medium of Stropharia stropharia, and then cultivate the inoculated fermenter at 26°C for 8 days; the stropharia The preparat...
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