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Use of Klebsiella pneumoniae M1 for degradation of agricultural waste

A Klebsiella, agricultural waste technology, applied to the removal of solid waste, methods based on microorganisms, bacteria, etc., can solve the problem of low enzyme activity, achieve rapid value-added, significant lignin degradation ability, and high-efficiency degradation Effect

Active Publication Date: 2019-01-18
海林市中农国泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of fungi to degrade lignocellulose generally has the problem of low enzyme activity

Method used

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  • Use of Klebsiella pneumoniae M1 for degradation of agricultural waste
  • Use of Klebsiella pneumoniae M1 for degradation of agricultural waste
  • Use of Klebsiella pneumoniae M1 for degradation of agricultural waste

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specific Embodiment approach 1

[0025] Specific embodiment 1: A lignocellulosic material high-efficiency degradation bacterium M1 of this embodiment, which is Klebsiella pneumoniae M1, is deposited in the General Microbiology Center of the China Microbial Culture Collection Management Committee, and the preservation address It is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The preservation date is December 12, 2014, and the preservation number is CGMCC No. 10162.

[0026] The Klebsiella pneumoniae M1 of this embodiment is a gram-negative bacterium. The bacterial cell of this strain is rod-shaped, and the cell size is 0.62~0.71×1.5~1.6μm, does not form spores, and has no flagella. , With a capsule; on the LB medium, a round, opaque, yellow, smooth protrusion and neat edge colony (such as figure 1 Shown).

[0027] The physiological and biochemical reaction results of this strain total 20 items and 30 indicators; combined with the morphological and physiological and biochemical reaction results, the...

specific Embodiment approach 2

[0093] Specific embodiment 2: The application of a lignocellulosic material high-efficiency degrading bacterium M1 of this embodiment is used for degrading agricultural waste, especially for degrading lignocellulosic materials in bag material, fungus fertilizer and corn stalks .

[0094] The following functional tests are performed on the strain of the present invention:

[0095] The Klebsiella pneumoniae M1 of the specific embodiment was tested for the weight loss rate and the degradation of lignin, cellulose and hemicellulose in bagged fungus waste and corn stalks to verify its unique functions. details as follows:

[0096] 1. Determination of weight loss rate of bag material fungus waste and corn stalk

[0097] 1.1 Corn stover meal

[0098] The corn stalks were taken from the experimental field of our laboratory on the Hulan campus of Heilongjiang University, Harbin, Heilongjiang Province in October 2012, dried, crushed through a 40-mesh sieve, and set aside.

[0099] 1.2 Bags of fu...

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Abstract

The invention relates to use of Klebsiella pneumoniae M1 for degradation of agricultural wastes. Klebsiella pneumoniae (Klebsiella pneumoniae) M1, deposited at the General Microbial Center of China Microbial Culture Collection Management Committee on December 12, 2014, accession number CGMCC No. 10162. The strain of the invention has the advantages of rapid multiplication, strong adaptability andwide application. The weight loss rate of Auricularia auricula waste after fermentation by Klebsiella pneumoniae (M1) was over 31%, The weight loss rate of corn stalk fermented by Klebsiella pneumoniae (Klebsiella pneumoniae) M1 was over 30%.

Description

[0001] This application is a divisional application of the following patent applications, the original application number: 201510862142.3, the filing date: 2015-12-01, the title of the invention: a strain of high-efficiency lignocellulose degradation bacteria M1 and its application. Technical field [0002] The invention relates to a strain of Klebsiella pneumoniae M1 and its use in degrading agricultural waste. Background technique [0003] my country is a large agricultural country, and the amount of agricultural waste generated is extremely large. Due to the limitation of economic benefits and technology popularization, most of agricultural waste is extensively and inefficiently used and is in a serious state of idleness, causing waste of resources and environmental pollution. Waste has become the largest in China. Source of pollution. However, there is a large amount of lignocellulose in agricultural waste, which urgently needs to be developed and utilized. [0004] Lignin is th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09B3/00C12R1/22
Inventor 杨微刘春光杨峰山孙永帅吴奇王鲲鹏王庆华张慧魏才强曲金玲于文全
Owner 海林市中农国泰生物科技有限公司