Water-in-oil transparent crude emulsion with phase separation and application thereof

A water-in-oil, coarse-emulsion technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as pollution and increased probability of false positives

Inactive Publication Date: 2019-01-18
因纽美瑞克斯公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This approach will very likely contaminate subsequent experiments, greatl

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  • Water-in-oil transparent crude emulsion with phase separation and application thereof
  • Water-in-oil transparent crude emulsion with phase separation and application thereof
  • Water-in-oil transparent crude emulsion with phase separation and application thereof

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preparation example Construction

[0080] The preparation method of transparent macroemulsion droplet among the present invention, described transparent macroemulsion droplet is the stable spherical particle that above-mentioned water phase liquid is emulsified and dispersed in oil phase and forms, and its preparation method generally comprises the following steps: (1) respectively Prepare water phase (containing refractive index enhancer, etc.) and oil phase (lipophilic surfactant) liquid, (2) emulsify and disperse to form transparent coarse emulsion droplets.

[0081] The method of emulsifying and dispersing the water phase into the oil phase to form droplets can be oscillating emulsification, micro-flow cross co-flow method or T-shaped flow channel droplet method or centrifugal droplet emulsification in the prior art (such as reference [10]). With these methods, droplets with adjustable diameter and good uniformity can be obtained.

[0082] In addition, different oil phases can be selected for different drop...

Embodiment 1

[0090] In order to verify the appropriate concentration of refractive index enhancer, Gelest DMS-T01.5 silicone oil and surfactant DowCorning ES5612 were prepared according to the mass ratio of 19:1, mixed evenly and centrifuged at 20,000rcf for 10 minutes to obtain the supernatant for use emulsified oil in the next step. Betaine in the water phase is a refractive index enhancer. The volume of the water phase of each sample is 20 μL, and 240 μL of the above-mentioned prepared oil is added to the system. figure 1 Shown from left to right is the situation of adding 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0 moles of refractive index enhancers respectively (the concentration here refers to the concentration in the finally formed water-oil mixture) . Depend on figure 1 It can be seen that as the concentration of the refractive index enhancer increases, the transparency first increases and then decreases, and is most transparent when the concentration of the refractive index enhancer...

Embodiment 2

[0092] The difference from Example 1 is that glycine is used as the refractive index enhancer. Glycine solutions with different concentrations (0.5, 1, 1.5 mol / liter) were centrifuged to form coarse emulsion droplets. As the concentration of glycine increases, the transparency first increases and then decreases, and the most transparent is when the concentration of the refractive index enhancer is 2.8 moles per liter.

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Abstract

Provided are a water-in-oil transparent crude emulsion with phase separation and application thereof. The transparent crude emulsion isobtained by emulsifying and dispersing an aqueous phase and an oil phase, wherein the aqueous phase liquid droplets dispersed in the oil phase have an average diameter of not less than 0.2 [mu]m, and an application thereof; And a refractive index reinforcing agentis also dissolved in the aqueous phase, and the refractive index reinforcing agent component accounts for not less than 20% by mass in the whole aqueous phase; And a surfactant with HBL value not greater than 8 is also dissolved in the oil phase; Furthermore, the absolute value of the difference in refractive index between the aqueous phase and the oil phase does not exceed 0.1. By controlling thecomposition of both the water phase and the liquid phase of the water-in-oil coarse emulsion used for phase separation, in particular by adjusting the refractive index of the water phase in the coarse emulsion droplets, the coarse emulsion droplets are transparent, so that light rays can pass through the shallow transparent droplets to reach the deep droplets, thereby realizing in-situ sealed imaging detection of the deep droplets of the coarse emulsion.

Description

technical field [0001] The invention belongs to the technical field of digital chain enzyme reaction detection, and more specifically, relates to a phase-isolated water-in-oil transparent coarse emulsion and its application. The phase-isolated water-in-oil transparent coarse emulsion is especially a water-oil two-phase It is a stable transparent macroemulsion for substance isolation, and digital quantitative detection can be realized by using the transparent macroemulsion system, and the corresponding detection method can especially realize high-specificity digital closed nucleic acid quantitative detection. Background technique [0002] Digital chain enzyme reaction is currently the most sensitive and accurate nucleic acid quantification method in the world. Among them, digital detection refers to the separation of solutions or suspensions containing target substances such as nucleic acid, protein and other biomacromolecules, or bacteria, cells, etc., into independent react...

Claims

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Application Information

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IPC IPC(8): C12Q1/6851
CPCC12Q1/6851C12Q2527/125C12Q2563/159C12Q2561/101C12Q1/686C12Q1/6806C12Q1/6848
Inventor 费鹏廖珮宇姜梦成张芳丽聂俊
Owner 因纽美瑞克斯公司
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