Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Water-in-oil transparent crude emulsion with phase separation and application thereof

A water-in-oil, coarse-emulsion technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as pollution and increased probability of false positives

Inactive Publication Date: 2019-01-18
因纽美瑞克斯公司
View PDF12 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This approach will very likely contaminate subsequent experiments, greatly increasing the probability of false positives in subsequent experiments

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Water-in-oil transparent crude emulsion with phase separation and application thereof
  • Water-in-oil transparent crude emulsion with phase separation and application thereof
  • Water-in-oil transparent crude emulsion with phase separation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0080] The preparation method of transparent macroemulsion droplet among the present invention, described transparent macroemulsion droplet is the stable spherical particle that above-mentioned water phase liquid is emulsified and dispersed in oil phase and forms, and its preparation method generally comprises the following steps: (1) respectively Prepare water phase (containing refractive index enhancer, etc.) and oil phase (lipophilic surfactant) liquid, (2) emulsify and disperse to form transparent coarse emulsion droplets.

[0081] The method of emulsifying and dispersing the water phase into the oil phase to form droplets can be oscillating emulsification, micro-flow cross co-flow method or T-shaped flow channel droplet method or centrifugal droplet emulsification in the prior art (such as reference [10]). With these methods, droplets with adjustable diameter and good uniformity can be obtained.

[0082] In addition, different oil phases can be selected for different drop...

Embodiment 1

[0090] In order to verify the appropriate concentration of refractive index enhancer, Gelest DMS-T01.5 silicone oil and surfactant DowCorning ES5612 were prepared according to the mass ratio of 19:1, mixed evenly and centrifuged at 20,000rcf for 10 minutes to obtain the supernatant for use emulsified oil in the next step. Betaine in the water phase is a refractive index enhancer. The volume of the water phase of each sample is 20 μL, and 240 μL of the above-mentioned prepared oil is added to the system. figure 1 Shown from left to right is the situation of adding 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0 moles of refractive index enhancers respectively (the concentration here refers to the concentration in the finally formed water-oil mixture) . Depend on figure 1 It can be seen that as the concentration of the refractive index enhancer increases, the transparency first increases and then decreases, and is most transparent when the concentration of the refractive index enhancer...

Embodiment 2

[0092] The difference from Example 1 is that glycine is used as the refractive index enhancer. Glycine solutions with different concentrations (0.5, 1, 1.5 mol / liter) were centrifuged to form coarse emulsion droplets. As the concentration of glycine increases, the transparency first increases and then decreases, and the most transparent is when the concentration of the refractive index enhancer is 2.8 moles per liter.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Provided are a water-in-oil transparent crude emulsion with phase separation and application thereof. The transparent crude emulsion isobtained by emulsifying and dispersing an aqueous phase and an oil phase, wherein the aqueous phase liquid droplets dispersed in the oil phase have an average diameter of not less than 0.2 [mu]m, and an application thereof; And a refractive index reinforcing agentis also dissolved in the aqueous phase, and the refractive index reinforcing agent component accounts for not less than 20% by mass in the whole aqueous phase; And a surfactant with HBL value not greater than 8 is also dissolved in the oil phase; Furthermore, the absolute value of the difference in refractive index between the aqueous phase and the oil phase does not exceed 0.1. By controlling thecomposition of both the water phase and the liquid phase of the water-in-oil coarse emulsion used for phase separation, in particular by adjusting the refractive index of the water phase in the coarse emulsion droplets, the coarse emulsion droplets are transparent, so that light rays can pass through the shallow transparent droplets to reach the deep droplets, thereby realizing in-situ sealed imaging detection of the deep droplets of the coarse emulsion.

Description

technical field [0001] The invention belongs to the technical field of digital chain enzyme reaction detection, and more specifically, relates to a phase-isolated water-in-oil transparent coarse emulsion and its application. The phase-isolated water-in-oil transparent coarse emulsion is especially a water-oil two-phase It is a stable transparent macroemulsion for substance isolation, and digital quantitative detection can be realized by using the transparent macroemulsion system, and the corresponding detection method can especially realize high-specificity digital closed nucleic acid quantitative detection. Background technique [0002] Digital chain enzyme reaction is currently the most sensitive and accurate nucleic acid quantification method in the world. Among them, digital detection refers to the separation of solutions or suspensions containing target substances such as nucleic acid, protein and other biomacromolecules, or bacteria, cells, etc., into independent react...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6851
CPCC12Q1/6851C12Q2527/125C12Q2563/159C12Q2561/101C12Q1/686C12Q1/6806C12Q1/6848
Inventor 费鹏廖珮宇姜梦成张芳丽聂俊
Owner 因纽美瑞克斯公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com