Gene detection probe group and screening method for 50 kinds of hereditary diseases of newborn

Abstract

The invention discloses a gene detection probe group and a screening method for 50 kinds of genetic diseases of newborns, comprising a detection probe box, wherein the detection probe box is internally provided with a DNA probe, an RNA probe and a label probe; the specific detection method comprises the following steps of: S1, cleaning tissue of newborns; S2, then one membrane of 0.02 mm being cut, and the membrane being soaked in 100% methanol for 10s, and then the membrane becoming translucent, and then soaked in transfer buffer. Six filter papers of the same size being cut, and the blood membrane being shaken in 100% methanol for 5min; S3, labeling it with a labeling probe, and then inserting the DNA probe into the detected membrane, and simultaneously putting isotope, biotin or fluorescent dye into the membrane; S4, inserting RNA probe into the membrane, and injecting SP6 RNA polymerase and T7 RNA polymerase into the membrane at the same time for detection; S5, analysis of the synthetic results of color rendering calculation. The method of the invention has the advantages of accurate detection, low cost and short detection period, and is worth popularizing.

Description

technical field [0001] The invention relates to the technical field of medical identification, in particular to a probe set and a screening method for detecting genes of 50 kinds of genetic diseases in newborns. Background technique [0002] Gene is the basic unit of heredity. It is a DNA or RNA sequence carrying genetic information. Through replication, the genetic information is passed on to the next generation, and it guides the synthesis of proteins to express the genetic information it carries, thereby controlling the expression of the traits of individual organisms. Genetic testing is a technology for detecting DNA through blood, other body fluids, or cells. It is to take the peripheral venous blood or other tissue cells of the tested person, amplify their genetic information, and use specific equipment to detect the DNA molecules in the tested person's cells. It is a method to detect and analyze the gene types and gene defects contained in it and whether its expressio...

AI Technical Summary

Problems solved by technology

[0003] At present, many children in China are born with genetic disease genes. These genes are difficult to detect, and long-term latency can easily affect children's development. Traditional detection methods are relatively backward, and most of them are detected by blood tests, which are less accurate. Above, here we propose 50 newborn genetic disease gene detection probe sets and screening methods

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