Method for using castanea mollissima polysaccharides to improve animal semen quality

A technology of chestnut polysaccharide and semen is applied in the direction of animal feed, application, additional food elements, etc., to achieve the effects of improving reproductive system, improving semen quality, and improving semen quality.

Active Publication Date: 2019-02-01
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether chestnut polysaccharides have the effect of improving semen quality is still unknown, and there is no document disclosing the method of using chestnut polysaccharides to improve semen quality

Method used

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  • Method for using castanea mollissima polysaccharides to improve animal semen quality
  • Method for using castanea mollissima polysaccharides to improve animal semen quality
  • Method for using castanea mollissima polysaccharides to improve animal semen quality

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: preparation chestnut polysaccharide

[0031] 1) Pick fresh and mature chestnuts, place them in an oven, dry them, peel them, crush and dry them with a grinder, degrease, pass through a 100-mesh sieve, weigh them, add ultra-pure water at a material-to-liquid ratio of 25g / L, and use a homogenizer Mix well, and then ultrasonically extract in an ultrasonic instrument with a power of 850W at 35°C for 1 hour;

[0032] 2) The extract obtained in step (1) was decolorized with activated carbon at 45°C for 1 hour, then the protein in the solution was repeatedly removed by the Sevage method, and then centrifuged at 11000r / min for 20 minutes, the precipitate was discarded, and the supernatant was recovered;

[0033] 3) Use a rotary evaporator to concentrate the supernatant obtained in step (2) until 80% of the supernatant is evaporated. At this time, the concentrated solution is precipitated with 100% alcohol, and the obtained precipitate is freeze-dried, and the obta...

Embodiment 2

[0036] Embodiment 2: Mouse feeding test

[0037] 210 3-week-old mice (with uniform body weight) of the healthy ICR strain were randomly assigned to 7 groups (30 / group). The mice were eating and drinking normally, and were raised in a unified SPF rat room . Take a certain amount of powder obtained in step 5) of Example 1 according to the design in Table 1 every day, convert the amount of powder needed to be used according to the amount of chestnut polysaccharide designed in the experimental group, dissolve it in purified water, and prepare the required chestnut polysaccharide solution. Each mouse was fed with chestnut polysaccharide solution matching its body weight by intragastric administration on time every day. Each mouse was gavaged with 0.1 mL chestnut polysaccharide solution. After 5 weeks of gavage, the semen of the mice was collected, and the sperm motility and density were analyzed with a semen quality analyzer. The test results are shown in Table 1:

[0038] Tabl...

Embodiment 3

[0041] Embodiment 3: Pig feeding test

[0042]In the cooperative experimental base of our school, 70 healthy weaned (40-day-old) boars were selected and randomly divided into 7 groups (10 heads / group). All pigs are in a large breeding room, eating and drinking normally, and are under unified management. Take a certain amount of powder obtained in step 5) of Example 1 according to the design in Table 2 every day, and convert the amount of powder that needs to be used according to the amount of chestnut polysaccharide designed in the experimental group. Fully mix the weighed animal concentrate and chestnut polysaccharide powder evenly. Feed the prepared feed concentrate containing chestnut polysaccharide to pigs on time every day. Feed until 6 months old, collect the semen of the pigs, and use the semen quality analyzer to analyze the sperm motility and density.

[0043] Table 2 Effect of pig supplementation with chestnut polysaccharide on semen quality

[0044] Dai...

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Abstract

The present invention relates to a method for using castanea mollissima polysaccharides to improve animal semen quality. The castanea mollissima polysaccharides are supplemented by a manner of conducting gavage or adding into feeds every day after weaning of male mammals; an ingesting dosage for the small-sized male mammals is 0.1-100 mg / kg body weight; and an ingestion dosage for the middle-sizedmale mammals is 0.5-200 mg / kg feeds. The castanea mollissima polysaccharides are freeze-dried powder obtained by further purification after ultrasonic extraction. The method for using ultrasonic to extract castanea mollissima polysaccharides and conducting the further purification is provided for the first time; and the castanea mollissima polysaccharides supplement the feeds for the male mammalsand can significantly improve the semen quality of the animals. Supplementary feeding timing starts after the weaning of the male mammals. A technical scheme is to enable the male mammals to better establish a reproductive system, fully exploit genetic potential of the male animals, and expand use scope of the castanea mollissima polysaccharides.

Description

technical field [0001] The invention relates to the field of animal reproductive physiology and reproduction technology, in particular to a method for improving the quality of animal semen by using chestnut polysaccharide. Background technique [0002] In recent years, due to the excessive or incorrect application of pesticides and fertilizers, environmental pollution, climate change and other reasons, the sperm motility of male animals has decreased, the concentration of sperm has decreased, and the rate of sperm deformity has increased, resulting in a substantial increase in the rate of male infertility. This is a worldwide problem that has attracted widespread attention from all over the world. However, there is no good method to improve semen quality to treat male infertility. Therefore, scientists are looking for effective drugs or methods to improve the semen quality of male animals. [0003] Chestnut (Castanea mollissima BL.), also known as: chestnut, chestnut, Kui ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/163C08B37/00
CPCA23K20/163C08B37/0003
Inventor 沈伟孙中义赵勇李兰于帅
Owner QINGDAO AGRI UNIV
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