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HCG detection reagent, test strip and use method thereof

A reagent and detection line technology, which is applied in the field of immunochromatographic test paper for detecting HCG, can solve the problems of large influence of environmental temperature, complicated detection process, narrow detection range, etc., and achieve the effect of improving reliability and accurate and reliable detection results

Active Publication Date: 2019-02-01
SICHUAN MACCURA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, the introduction of the temperature compensation module and the detector will inevitably lead to an increase in cost and a complication of the detection process
[0010] It is worth noting that none of the above solutions solves the two problems of narrow detection range and great influence of ambient temperature

Method used

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  • HCG detection reagent, test strip and use method thereof
  • HCG detection reagent, test strip and use method thereof
  • HCG detection reagent, test strip and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The preparation of embodiment 1 reaction reagent

[0067] Dissolve FITC in 10mM PBS solution at a certain concentration, take a certain amount of mouse anti-β-HCG monoclonal antibody 4 (Sichuan Mike Biological New Material Technology Co., Ltd., XCL1401) into the dialysis bag, put the dialysis bag into the FITC solution Place it on a magnetic stirrer, and stir and dialyze overnight in the dark. After dialysis, take out FITC-mouse anti-β-HCG monoclonal antibody 4 and store at 4°C.

[0068] Take an appropriate volume of fluorescent microspheres (JSR, F7655S) solution and add EDC (0.1mg / 1mg fluorescent microspheres) activator to activate for 30min, then add FITC-mouse anti-β-HCG single Antibody 4 was cloned, stirred and reacted for 1 hour at a constant speed, then a blocking agent was added, and the reaction was terminated for 30 minutes, and finally a complex of FITC~mouse anti-β-HCG monoclonal antibody 4~fluorescent microspheres was obtained.

[0069] Take an appropriat...

Embodiment 2

[0071] Embodiment 2 detects the preparation of test paper

[0072] Dilute the mouse anti-β-HCG monoclonal antibody 3 (Sichuan Mike Biological New Material Technology Co., Ltd., XCL1400) to 2 mg / mL with coating buffer; Co., Ltd., XCL1510) was diluted to 2 mg / mL, goat anti-rabbit IgG was diluted to 1 mg / mL, and anti-FITC monoclonal antibody was mixed with goat anti-rabbit IgG at a ratio of 1:1. Draw the mouse anti-β-HCG monoclonal antibody 3 on the detection line with a film drawer, put the anti-FITC monoclonal antibody and goat anti-rabbit IgG quality control line (spray volume: 1 μL / cm), and dry at 55°C for 3 days .

Embodiment 3

[0073] Embodiment 3 detection method

[0074] Take 20 μL sample (or quality control product) and add it to 280 μL / tube of reaction solution, mix well, add 90 μL of the mixed solution into the sample hole of the test card, and after chromatography at room temperature for 15 minutes, insert the test card into the socket of the immunofluorescence detector. In the loading hole, the instrument will automatically scan the test card and calculate the results.

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Abstract

The invention relates to an immunochromatographic test strip for HCG detection. A detection line and a quality control line are sequentially disposed on the membrane of the immunochromatographic teststrip along the flow direction of a sample, wherein the detection line is coated with a first [Beta]-HCG monoclonal antibody, and the quality control line is coated with an antibody that binds to non-human IgG and an antibody that binds to a fluorescent label. The invention also relates to a corresponding reagent and a preparation method, and a method for detecting HCG. The method for detecting HCG can expand the detection range and improve the influence of the ambient temperature when detecting HCG.

Description

technical field [0001] The invention relates to the field of immunodetection, in particular to an immunochromatographic test paper for detecting HCG. Background technique [0002] In 1927, Aschheim and zondek first discovered human chorionic gonadotropin (HumanChorionic Gonadotropin, HCG) in the urine of pregnant women. HCG is a glycoprotein secreted by the trophoblast cells of the placenta. It is composed of α and β dimer glycoproteins and plays a very important role in maintaining normal pregnancy. The α subunit is basically similar to FSH (follicle-stimulating hormone), LH (luteinizing hormone) and TSH (thyroid-stimulating hormone) secreted by the pituitary gland, so they can cross-react with each other, while the structure of the β subunit is different. β-HCG is similar in structure to β-LH, but the last 24 amino acid extensions do not exist in β-LH. [0003] Human chorionic gonadotropin begins to be secreted on the 8th to 10th day after conception. In the first 3 mon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/76G01N33/577
CPCG01N33/577G01N33/76G01N2333/59
Inventor 李军赵书阁陈维刘东泽
Owner SICHUAN MACCURA BIOTECH CO LTD
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